Results 71 to 80 of about 599,955 (287)

Crucial parameters for precise copy number variation detection in formalin‐fixed paraffin‐embedded solid cancer samples

Molecular Oncology, EarlyView.
This study shows that copy number variations (CNVs) can be reliably detected in formalin‐fixed paraffin‐embedded (FFPE) solid cancer samples using ultra‐low‐pass whole‐genome sequencing, provided that key (pre)‐analytical parameters are optimized.
Hanne Goris, Vasiliki Siozopoulou, Léon C van Kempen, Anne Sieben, Ella Roelant, Stig Hellemans, Elyne Backx, Laure Sorber, Koen De Winne, Senada Koljenović, Karen Zwaenepoel   +10 more
wiley   +1 more source

Phenotypic and genotypic characterization of single circulating tumor cells in the follow‐up of high‐grade serous ovarian cancer

Molecular Oncology, EarlyView.
Single circulating tumor cells (sCTCs) from high‐grade serous ovarian cancer patients were enriched, imaged, and genomically profiled using WGA and NGS at different time points during treatment. sCTCs revealed enrichment of alterations in Chromosomes 2, 7, and 12 as well as persistent or emerging oncogenic CNAs, supporting sCTC identity.
Carolin Salmon, Rui P. L. Neves, Nikolas H. Stoecklein, Sven‐Thorsten Liffers, Jens Siveke, Jan D. Kuhlmann, Pauline Wimberger, Paul Buderath, Rainer Kimmig, Sabine Kasimir‐Bauer   +9 more
wiley   +1 more source

Comparison of different sequencing techniques for identification of SARS-CoV-2 variants of concern with multiplex real-time PCR.

PLoS ONE, 2022
As different SARS-CoV-2 variants emerge and with the continuous evolvement of sub lineages of the delta variant, it is crucial that all countries carry out sequencing of at least >1% of their infections, in order to detect emergence of variants with ...
Diyanath Ranasinghe, Tibutius Thanesh Pramanayagam Jayadas, Deshni Jayathilaka, Chandima Jeewandara, Osanda Dissanayake, Dinuka Guruge, Dinuka Ariyaratne, Dumni Gunasinghe, Laksiri Gomes, Ayesha Wijesinghe, Ruwan Wijayamuni, Gathsaurie Neelika Malavige   +11 more
doaj   +1 more source

DNA methylation profiling of primary neuroblastoma tumors using methyl-CpG-binding domain sequencing [PDF]

, 2016
Comprehensive genome-wide DNA methylation studies in neuroblastoma (NB), a childhood tumor that originates from precursor cells of the sympathetic nervous system, are scarce. Recently, we profiled the DNA methylome of 102 well-annotated primary NB tumors
Decock, Anneleen, Ongenaert, Maté, Speleman, Franki, Van Criekinge, Wim, Vandesompele, Jo   +4 more
core   +1 more source

Microbiome Profiling by Illumina Sequencing of Combinatorial Sequence-Tagged PCR Products

PLoS ONE, 2010
28 pages, 13 ...
Gloor, Gregory B., Hummelen, Ruben, Macklaim, Jean M., Dickson, Russell J., Fernandes, Andrew D., MacPhee, Roderick, Reid, Gregor   +6 more
openaire   +7 more sources

Circular RNA expression landscapes in myelodysplastic neoplasms: Associations with mutational signatures and disease progression

Molecular Oncology, EarlyView.
In this explorative study, the abundance of circular RNA molecules in bone marrow stem cells was found to be elevated in patients with high‐risk myelodysplastic neoplasms, and to be associated with an increased risk of progression to acute myeloid leukemia.
Eileen Wedge, Morten Tulstrup, Gabriele Todisco, Pedro Moura, Christophe Côme, Jakob Werner Hansen, Jakob Schmidt Jespersen, Balthasar Schlotmann, Maria Creignou, Mette Dahl, Claudia Schöllkopf, Klas Raaschou‐Jensen, Krister Wennerberg, Bo Porse, Joachim Weischenfeldt, Eva Hellström‐Lindberg, Lasse Sommer Kristensen, Kirsten Grønbæk   +17 more
wiley   +1 more source

A cancer cell-line titration series for evaluating somatic classification. [PDF]

, 2015
BackgroundAccurate detection of somatic single nucleotide variants and small insertions and deletions from DNA sequencing experiments of tumour-normal pairs is a challenging task.
Beck, Timothy, Brown, Andrew MK, Denroche, Robert E, McPherson, John D, Mullen, Laura, Stein, Lincoln, Timms, Lee, Yung, Christina K   +7 more
core   +2 more sources

Colorectal cancer‐derived FGF19 is a metabolically active serum biomarker that exerts enteroendocrine effects on mouse liver

Molecular Oncology, EarlyView.
Meta‐transcriptome analysis identified FGF19 as a peptide enteroendocrine hormone associated with colorectal cancer prognosis. In vivo xenograft models showed release of FGF19 into the blood at levels that correlated with tumor volumes. Tumoral‐FGF19 altered murine liver metabolism through FGFR4, thereby reducing bile acid synthesis and increasing ...
Jordan M. Beardsley, Michael W. Rohr, Joseph A. Goode, Sai Preethi Nakkina, Jignesh G. Parikh, Deborah A. Altomare   +5 more
wiley   +1 more source

Genome sequencing of the extinct Eurasian wild aurochs, Bos primigenius, illuminates the phylogeography and evolution of cattle [PDF]

, 2015
Background Domestication of the now-extinct wild aurochs, Bos primigenius, gave rise to the two major domestic extant cattle taxa, B. taurus and B. indicus.
A Achilli, A Achilli, A Esteve-Codina, A Gotherstrom, A Seguin-Orlando, A Vaysse, A Winter, AE Minoche, AJ Amaral, Alison Murphy, Amanda J. Lohan, Andrew T. Chamberlain, AV Zimin, B Grisart, B Grisart, BP Lewis, Brendan J. Loftus, C Gamba, C Glaser, Ceiridwen J. Edwards, CG Elsik, Charles Spillane, CJ Edwards, CJ Edwards, CJ Edwards, CJ Rubin, CJ Stevens, CM Leu, CS Troy, D Reich, Daniel G. Bradley, David A. Magee, David E. MacHugh, DE MacHugh, DG Bradley, DM Larkin, DP Toews, E Palkopoulou, E Svensson, EJ McTavish, EY Durand, H Jonsson, H Jonsson, H Li, H Zhang, HD Daetwyler, J Clutton-Brock, J Diamond, J Kantanen, J Lenstra, J Schibler, JA Guerra-Assuncao, JD Vigne, JE Decker, JK Pickrell, JK Pritchard, JS Pedersen, K Prufer, KA Moutou, Kévin Rue-Albrecht, L Orlando, L Perez-Pardal, LK Matukumalli, M Gautier, M Hofreiter, M Li, M Meyer, M Raghavan, M Rasmussen, M Schubert, MA DePristo, MA Greagg, MA Groenen, Mark T. Donoghue, Martin Braud, Matthew D. Teasdale, MJ Montague, N Murakami, N Patterson, NA Rosenberg, O Smith, Paul A. McGettigan, R Bollongino, R Chen, RA Gibbs, RE Green, RE Green, RH Meadow, RR Hudson, RT Loftus, S Bonfiglio, S Bonfiglio, S Bonfiglio, S Guindon, S Koks, S Paabo, S Qanbari, S Sawyer, Shuaishuai Tai, Stephen D E Park, Steven Schroeder, Tad S. Sonstegard, TH Lee, W McLaren, Y Benjamini, Yuan Liu   +105 more
core   +1 more source

CRISPR-hybrid N11 Illumina sequencing data

, 2023
FASTQ files used in data analysis for "CRISPR-Hybrid: A CRISPR-Mediated Intracellular Directed Evolution Platform for RNA Aptamers".
openaire   +1 more source