Results 71 to 80 of about 11,169 (113)

Accelerating SARS-CoV-2 genomic surveillance in a routine clinical setting with nanopore sequencing

International Journal of Medical Microbiology
Background: SARS-CoV-2 genomic analysis has been key to the provision of valuable data to meet both epidemiological and clinical demands. High-throughput sequencing, generally Illumina-based, has been necessary to ensure the widest coverage in global ...
Sergio Buenestado-Serrano, Marta Herranz, Álvaro Otero-Sobrino, Andrea Molero-Salinas, Cristina Rodríguez-Grande, Amadeo Sanz-Pérez, María José Durán Galván, Pilar Catalán, Roberto Alonso, Patricia Muñoz, Laura Pérez-Lago, Darío García de Viedma   +11 more
doaj   +1 more source

Barcode Sequencing by Illumina NGS v1

This protocol details the extraction of genomic DNA from cells and barcode sequencing by NGS.
Nicholas A. Popp, Melinda K. Wheelock, Raining Wang, Rachel L. Powell, Douglas M. Fowler   +4 more
openaire   +1 more source

Illumina Sequencing [PDF]

, 2020
openaire   +1 more source

Full-length 16S rRNA gene sequencing by PacBio improves taxonomic resolution in human microbiome samples

BMC Genomics
Background Sequencing variable regions of the 16S rRNA gene (≃300 bp) with Illumina technology is commonly used to study the composition of human microbiota.
Elena Buetas, Marta Jordán-López, Andrés López-Roldán, Giuseppe D’Auria, Llucia Martínez-Priego, Griselda De Marco, Miguel Carda-Diéguez, Alex Mira   +7 more
doaj   +1 more source

Primed and ready: nanopore metabarcoding can now recover highly accurate consensus barcodes that are generally indel-free

BMC Genomics
Background DNA metabarcoding applies high-throughput sequencing approaches to generate numerous DNA barcodes from mixed sample pools for mass species identification and community characterisation.
Jia Jin Marc Chang, Yin Cheong Aden Ip, Wan Lin Neo, Maxine A. D. Mowe, Zeehan Jaafar, Danwei Huang   +5 more
doaj   +1 more source

Comparative analysis of illumina and oxford nanopore sequencing platforms for 16S rRNA profiling of respiratory microbial communities

Scientific Reports
The respiratory microbiome plays a crucial role in health and disease, necessitating accurate characterization through high-throughput sequencing technologies.
Guillem Macip, Alba Soler-Comas, Andrea Palomeque, Ana Motos, Blanca Llonch, Joan Canseco-Ribas, Leticia Bueno-Freire, Davide Calabretta, Kasra Kiarostami, Roberto Cabrera, Miquel Ferrer, Antoni Torres, Laia Fernandez-Barat   +12 more
doaj   +1 more source

Evaluation of somatic copy number variation detection by NGS technologies and bioinformatics tools on a hyper-diploid cancer genome

Genome Biology
Background Copy number variation (CNV) is a key genetic characteristic for cancer diagnostics and can be used as a biomarker for the selection of therapeutic treatments.
Daniall Masood, Luyao Ren, Cu Nguyen, Francesco G. Brundu, Lily Zheng, Yongmei Zhao, Erich Jaeger, Yong Li, Seong Won Cha, Aaron Halpern, Sean Truong, Michael Virata, Chunhua Yan, Qingrong Chen, Andy Pang, Reyes Alberto, Chunlin Xiao, Zhaowei Yang, Wanqiu Chen, Charles Wang, Frank Cross, Severine Catreux, Leming Shi, Julia A. Beaver, Wenming Xiao, Daoud M. Meerzaman   +25 more
doaj   +1 more source

P597: All for One Clinical Genomics Network: Linking Canadian diagnostic laboratories to share genome-wide sequencing data to support rare disease diagnosis

Genetics in Medicine Open
E. Magda Price, Meredith Gillespie, Caitlin Chisholm, Ruben Attali, Talia Silver, Anna Szuto, GSO Study Team, Jacques Michaud, Dennis Bulman, Jordan Lerner-Ellis, Ma'n Zawati, Vincent Ferretti, Francois Bernier, Christian Marshall, Kym Boycott   +14 more
doaj   +1 more source

Candida auris sequencing by Illumina miSeq using Illumina DNA Prep v1

Candida auris sequencing is becoming more important for public health and surveillance. This protocol is designed to guide individuals experienced in sequencing in setting up a SOP for sequencing C. auris. This protocol is designed for Illumina short-read sequencing.
openaire   +1 more source

My Seq Illumina sequencer and Next Generation Sequencing technologies

, 2016
Overview of next generation sequencing (NGS) technologies:Illumina MySeqIllumina technologies comparison MySeq, NextSeq, HiSeq.PacBio.Ion Torrent.Oxford Nanopore.
openaire   +1 more source