Results 91 to 100 of about 463,626 (303)

Illumina sequencing plate2 [PDF]

, 2015
This file contains both forward (t2-UNIS_S1_L001_R1_001.fastq) and reverse (t2-UNIS_S1_L001_R2_001.fastq) Illumina read data and associated barcode mapping files.
Sunil Mundra (3283908), Mundra, Sunil, Kauserud, Håvard, Leho Tedersoo (106220), Halvorsen, Rune, Håvard Kauserud (3250542), Bahram, Mohammad, Eidesen, Pernille, Tedersoo, Leho, Pernille Eidesen (3297009), Rune Halvorsen (3250545), Mohammad Bahram (3292824)   +11 more
core   +1 more source

Illumina deep sequencing reads map. [PDF]

, 2020
RNA was extracted from samples by using TRIzol method (Invitrogen) and its degradation and contamination were monitored on 1% agarose gels. The cDNA synthesis was performed with 3 μg of total RNA using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB,
Felipe A. Dias (7408973), Carolina S. D. Vieira (8212254), Attilio Pane (5843135), Marcos Alcocer (8212260), Fei Sang (256927), Francis M. S. Saraiva (8212248), Ana Rossini (606876), Natália P. Nogueira (8212251), Marcia C. Paes (8212245), Vitor Lima Coelho (8212257)   +9 more
core   +1 more source

Solution-based targeted genomic enrichment for precious DNA samples

BMC Biotechnology, 2012
Background Solution-based targeted genomic enrichment (TGE) protocols permit selective sequencing of genomic regions of interest on a massively parallel scale.
Shearer Aiden, Hildebrand Michael S, Smith Richard JH   +2 more
doaj   +1 more source

Finding novel vulnerabilities of hypomorphic BRCA1 alleles

Molecular Oncology, EarlyView.
Synthetic lethality screens performed to identify novel vulnerabilities often model complete gene loss, thereby overlooking patient‐derived hypomorphic mutations. In this study, we have performed genome‐wide CRISPR screens on BRCA1 hypomorphic mutations, showing BRCA1I26A behaves like wild‐type, while BRCA1R1699Q mimics deficiency. Furthermore, we have
Anne Schreuder, Klaas de Lint, Hồ Mỹ Phúc Võ, Mariana M. Góis, Rosalie A. Kampen, Marta San Martin Alonso, Ilse Nootenboom, Veronica Garzero, Tiemen J. Wendel, Rob M. F. Wolthuis, Sylvie M. Noordermeer   +10 more
wiley   +1 more source

Longitudinal genome‐wide aneuploidy measurements in circulating cell‐free DNA to predict lack of benefit from pembrolizumab in patients with metastatic urothelial cancer

Molecular Oncology, EarlyView.
Many patients with urothelial cancer do not benefit from treatment with pembrolizumab, while at risk of severe side effects. Changes in the levels of circulating tumor DNA early during treatment, measured by a simple and affordable assay that can be easily implemented in the clinic, can be used as a prognostic tool to identify these patients.
Youssra Salhi, Stavros Makrodimitris, Sandra van Wilpe, Iris te Paske, Vanja de Weerd, Corine M. Beaufort, Hans M. Westgeest, Jens Voortman, Maureen J. B. Aarts, Maud Rijnders, Astrid A. M. van der Veldt, Ronald de Wit, Niven Mehra, Saskia M. Wilting, Debbie G. J. Robbrecht   +14 more
wiley   +1 more source

Addressing challenges in the production and analysis of illumina sequencing data [PDF]

BMC Genomics, 2011
Abstract Advances in DNA sequencing technologies have made it possible to generate large amounts of sequence data very rapidly and at substantially lower cost than capillary sequencing. These new technologies have specific characteristics and limitations that require either consideration during project design, or which must be addressed ...
Kircher, M., Heyn, P., Kelso, J. ; https://orcid.org/0000-0002-3618-322X   +2 more
openaire   +4 more sources

Nanopore duplex sequencing as an alternative to Illumina MiSeq sequencing for eDNA-based biomonitoring of coastal aquaculture impacts [PDF]

Metabarcoding and Metagenomics
Oxford Nanopore Technologies has recently launched a duplex sequencing strategy and announced an improved error rate which is in a similar order of magnitude as Illumina sequencing.
Thorsten Stoeck, Sven Nicolai Katzenmeier, Hans-Werner Breiner, Verena Rubel   +3 more
doaj   +3 more sources

MITF maintains genome stability in nonmelanocyte lineages

Molecular Oncology, EarlyView.
MITF is essential for melanocyte survival and acts as an oncogene in 10%–20% of melanomas. We show that MITF depletion causes genome instability in nonmelanocytic cells, leading to LATS2‐mediated P53 activation, cell cycle arrest, and apoptosis. This study highlights the role of MITF as a genome maintenance factor beyond the melanocyte lineage. Created
Drifa H. Gudmundsdottir, Adrián López García de Lomana, Thejus B. Venkatesh, Kritika Kirty, Snaevar Sigurdsson, Linda Vidarsdottir, Ramile Dilshat, Erla Sveinbjornsdottir, Snaedis Ragnarsdottir, Daniel H Magnusson, Maria R. Bustos, Eirikur Steingrimsson, Thorkell Gudjonsson, Stefan Sigurdsson   +13 more
wiley   +1 more source

Illumina-based transcriptome sequencing of mussel Mytilus coruscus mantle

浙江大学学报. 农业与生命科学版, 2015
Mollusk shell is an exoskeleton made up of organic mineral particles and its function is to protect the inner soft body from predators and environmental damage. Due to its superior mechanical properties, such as stiffness, fracture toughness, and tensile
Bao Linfei, Wang Xinxing, He Jianyu, Fan Meihua, Gao Peng, Liao Zhi   +5 more
doaj   +1 more source

Tumor B‐cell infiltration in platinum‐treated advanced muscle‐invasive urothelial carcinoma

Molecular Oncology, EarlyView.
Bladder tumors with higher pretreatment memory B‐cell infiltration were linked to longer survival after cisplatin chemotherapy, but not carboplatin. These tumors also showed more organized immune structures (tertiary lymphoid structures) and a shared pro‐inflammatory B‐cell‐rich community, suggesting that memory B cells may help identify patients most ...
Konrad Stawiski, Júlia Perera‐Bel, Alejo Rodriguez‐Vida, Nuria Juanpere, Jihyun Lee, Daniel E. Michaud, Jennifer L. Guerriero, Kent W. Mouw, Aristotelis Bamias, Filipe L. F. Carvalho, Joaquim Bellmunt   +10 more
wiley   +1 more source