Results 101 to 110 of about 463,626 (303)

PCR biases distort bacterial and archaeal community structure in pyrosequencing datasets [PDF]

, 2012
As 16S rRNA gene targeted massively parallel sequencing has become a common tool for microbial diversity investigations, numerous advances have been made to minimize the influence of sequencing and chimeric PCR artifacts through rigorous quality control ...
Lutgarde Raskin (143205), Ameet J. Pinto (143202), Lutgarde Raskin, Pinto Ameet J., Raskin, L., Ameet J Pinto, Raskin Lutgarde, Pinto, A.   +7 more
core   +1 more source

stilPCR increases the effective sequencing length of Illumina targeted next-generation sequencing.

PLoS ONE
Identifying pathogens, resistance-conferring mutations, and strain types through targeted amplicon sequencing is an important tool. However, due to the limitations of short read sequencing, many applications require the division of limited clinical ...
Jason D Limberis, Roland J Nagel, Soumitesh Chakravorty, Dena Block, Scott Dewell, Alina Nalyvayko, Zach Howard, Grant Theron, Rouxjeane Venter, John Z Metcalfe   +9 more
doaj   +1 more source

Loss of proton‐sensing TDAG8 increases tumor progression in mouse models of colon cancer

Molecular Oncology, EarlyView.
Loss of the pH‐sensing receptor TDAG8 accelerates colorectal cancer progression in mice. Animals lacking TDAG8 expression had increased tumor growth, DNA damage, and recruitment of tumor‐associated immune cells, including macrophages, neutrophils, and monocytes.
Ermanno Malagola, Yasmine Illi, Anna Bircher, Marijn Wilmink, Rachele F. Malagutti, Solenn Ottié, Cordelia Schuler, Pedro A. Ruiz, Cheryl de Vallière, Klaus Seuwen, Gerhard Rogler, Martin Hausmann   +11 more
wiley   +1 more source

Routine use of microbial whole genome sequencing in diagnostic and public health microbiology [PDF]

, 2012
This work is partly supported by funding from the Medical Research Council, the European Developing Country Clinical Trials Partnership, the Global Alliance for TB Drug Development, and the European Union.Whole genome sequencing (WGS) promises to be ...
Holden, MTG, Matthew T G Holden, Mark Farrington (148224), Cartwright, EJP, Matthew T. G. Holden (130258), Brown, Nicholas M., Sharon J Peacock, Bentley, SD, Ellington, Matthew J., Claudio U Köser, Parkhill, J, Stephen D. Bentley (137281), Stephen H Gillespie, Stephen D Bentley, Köser, CU, Bentley, Stephen D., Julian Parkhill (30393), Holden, Matthew T. G., Julian Parkhill, Peacock, Sharon J., Edward J. P. Cartwright (148213), Koeser, Claudio U., Peacock, SJ, Stephen H. Gillespie (148218), Edward J P Cartwright, Gordon Dougan, Matthew J. Ellington (148208), Farrington, M, Cartwright, Edward J. P., Dougan, Gordon, Nicholas M Brown, Parkhill, Julian, Mark Farrington, Gordon Dougan (30395), Farrington, Mark, Claudio U. Köser (148202), Gillespie, Stephen H., Matthew J Ellington, Brown, NM, Sharon J. Peacock (35782), Gillespie, SH, Ellington, MJ, Nicholas M. Brown (148222), Dougan, G   +43 more
core   +1 more source

Microbiome-based environmental monitoring of a dairy processing facility highlights the challenges associated with low microbial-load samples

npj Science of Food, 2021
Efficient and accurate identification of microorganisms throughout the food chain can potentially allow the identification of sources of contamination and the timely implementation of control measures.
Aoife J. McHugh, Min Yap, Fiona Crispie, Conor Feehily, Colin Hill, Paul D. Cotter   +5 more
doaj   +1 more source

Epigenetic silencing of the liver‐specific lncRNA LUNAR promotes liver cancer progression via NOTCH activation

Molecular Oncology, EarlyView.
LUNAR is a liver‐specific long noncoding RNA (lncRNA) that is highly expressed in normal liver but becomes epigenetically silenced in hepatocellular carcinoma through promoter hypermethylation. Loss of LUNAR is associated with NOTCH activation, epithelial–mesenchymal transition, and metastasis, whereas restoring LUNAR restrains metastatic progression ...
Se Ha Jang, Hyung Seok Kim, Geum Ok Baek, Moon Gyeong Yoon, Su In Lee, Jee‐Yeong Jeong, Ji Eun Han, Soon Sun Kim, Jae Youn Cheong, Jung Woo Eun   +9 more
wiley   +1 more source

VADER PylT Illumina sequencing data

, 2022
FASTQ files used in data analysis for "Virus-assisted directed evolution of enhanced suppressor tRNAs in mammalian cells."
openaire   +2 more sources

Removing noise from pyrosequenced amplicons [PDF]

, 2011
<b>Background</b><p></p> In many environmental genomics applications a homologous region of DNA from a diverse sample is first amplified by PCR and then sequenced.
Quince, Christopher, Marie Ollier, Decousus, Stephanie, Davenport, Russell J., Christopher Quince, Davenport Russell J, Quince Christopher, Ollier, Marie, Sandrine Viala, Saghir, Nagi,, Uhrhammer, Nancy, Saghir, Nagi, S El, Turnbaugh, Peter, Davenport, Russell J, Turnbaugh, Peter J., Lanzen Anders, Ponelle, Flora, Nancy Uhrhammer, Russell J Davenport, Lanzen, Anders, Uhrhammer, Nancy A., Axel Poulet, Privat, Maud, Peter J Turnbaugh, Maud Privat, Axel Perin, Lafarge, Laurence, Bidet, Yannick, Laurence Lafarge, Stephanie Decousus, Poulet, Axel, Viala, Sandrine, Yannick Bidet, Nagi S. El Saghir, Yves-Jean Bignon, Bignon, Yves-Jean, Turnbaugh Peter J, El-Saghir, Nagi S., Flora Ponelle, Anders Lanzen, Perin, Axel, Bignon, Yves Jean   +41 more
core   +1 more source

Interferon beta drives therapy resistance in a patient‐derived model of high‐grade serous ovarian cancer

Molecular Oncology, EarlyView.
Interferon type 1 (IFN‐1) production and signaling is associated with the acquisition of therapy resistance, following chronic DNA damage, via Interferon‐related DNA damage resistance signature (IRDS) gene expression. An alternative, DNA damage‐independent role of sustained IFN‐1 mediated resistance was identified and characterized by the emergence of ...
Ashlyn Conant, Tise Suzuki, Kiera McGivney, V. S. S. Abhinav Ayyadevara, Sharon Asariah, Jay Deng, Ethan Nyein, Jacqueline Coats, Gary Yu, Yevgeniya J. Ioffe, Christian Hurtz, Juli J. Unternaehrer   +11 more
wiley   +1 more source

Schematic of Illumina deep sequencing and analysis. [PDF]

, 2013
It includes sample preparation, cDNA library construction and Illumina sequencing, data analysis including assemble, blast, GO annotation, gene expression analysis, etc.
Yu Wang (12152), Jianjian Lv (492154), Baoquan Gao (492155), Ping Chen (89712), Jian Li (41607), Ping Liu (156158)   +5 more
core   +1 more source