Results 1 to 10 of about 23,294 (112)

Reliable investigation of DNA methylation using Oxford nanopore technologies [PDF]

Scientific Reports
Oxford Nanopore Technologies (ONT) offers an efficient and effective solution for studying DNA methylation. Both R9.4.1 and R10.4.1 ONT chemistries have been widely used to generate numerous DNA methylation data, making it inevitable to conduct cross-ONT-
Raj Doshi, Evan Kinnear, Sujan Chatterjee, Prasun Guha, Qian Liu   +4 more
doaj   +2 more sources

Benchmarking Illumina and Oxford Nanopore Technologies (ONT) sequencing platforms for whole genome sequencing of bacterial genomes and use in clinical microbiology [PDF]

BMC Medical Genomics
Background In microbial diagnostics, whole-genome sequencing (WGS) is used to address key questions such as species identification, presence of antimicrobial resistance genes (ARGs), virulence genes, and outbreak detection.
Srinithi Purushothaman, Tim Roloff, Adrian Egli, Helena MB Seth-Smith   +3 more
doaj   +3 more sources

Targeted haplotyping in pharmacogenomics using Oxford Nanopore Technologies’ adaptive sampling [PDF]

Frontiers in Pharmacology, 2023
Laurentijn Tilleman, Filip Van Nieuwerburgh, Dieter Deforce   +2 more
exaly   +2 more sources

Assembling Quality Genomes of Flax Fungal Pathogens from Oxford Nanopore Technologies Data [PDF]

Journal of Fungi (Basel, Switzerland), 2023
Nataliya V Melnikova, Alexey A Dmitriev, Liubov Povkhova   +2 more
exaly   +2 more sources

NanoForms: an integrated server for processing, analysis and assembly of raw sequencing data of microbial genomes, from Oxford Nanopore technology [PDF]

PeerJ, 2022
Background Next Generation Sequencing (NGS) techniques dominate today’s landscape of genetics and genomics research. Though Illumina still dominates worldwide sequencing, Oxford Nanopore is one of the leading technologies currently being used by ...
Anna Czmil, Michal Wronski, Sylwester Czmil, Marta Sochacka-Pietal, Michal Cmil, Jan Gawor, Tomasz Wołkowicz, Dariusz Plewczynski, Dominik Strzalka, Michal Pietal   +9 more
doaj   +2 more sources

Error correction enables use of Oxford Nanopore technology for reference-free transcriptome analysis

Nature Communications, 2021
Nanopore sequencing technologies applied to transcriptome analysis suffer from high error rates, limiting them largely to reference-based analyses. Here, the authors develop a computational error correction method for transcriptome analysis that reduces ...
Kristoffer Sahlin, Botond Sipos, Phillip L. James, Paul Medvedev   +3 more
doaj   +1 more source

Oncogene Concatenated Enriched Amplicon Nanopore Sequencing for rapid, accurate, and affordable somatic mutation detection

Genome Biology, 2021
We develop the Oncogene Concatenated Enriched Amplicon Nanopore Sequencing (OCEANS) method, in which variants with low variant allele frequency (VAFs) are amplified and subsequently concatenated for Nanopore Sequencing.
Deepak Thirunavukarasu, Lauren Y. Cheng, Ping Song, Sherry X. Chen, Mitesh J. Borad, Lawrence Kwong, Phillip James, Daniel J. Turner, David Yu Zhang   +8 more
doaj   +1 more source

Rapid Diagnosis of Mycobacterium marinum Infection by Next-Generation Sequencing: A Case Report

Frontiers in Medicine, 2022
We present the first report of histology- and culture-proven Mycobacterium marinum infection diagnosed by next-generation sequencing (NGS). It took <2 days to make a microbiological diagnosis using the Oxford Nanopore Technologies' MinION device ...
Fanfan Xing, Simon K. F. Lo, Yuanchao Ma, Jonathan Daniel Ip, Wan-Mui Chan, Meixun Zhou, Miaozi Gong, Susanna K. P. Lau, Patrick C. Y. Woo   +8 more
doaj   +1 more source

Comparative evaluation of sequencing platforms: Pacific Biosciences, Oxford Nanopore Technologies, and Illumina for 16S rRNA-based soil microbiome profiling [PDF]

Frontiers in Microbiology
Veselovsky V, Romanov M, Zoruk P, Larin A, Babenko V, Morozov M, Strokach A, Zakharevich N, Khamidova S, Danilova A, Vatlin A, Pavshintsev V, Chenguang F, Tsybizov D, Mitkin N, Galanova O, Zakharenko A, Golokhvast K, Klimina K.   +18 more
exaly   +2 more sources

Rapid diagnosis of fatal Nocardia kroppenstedtii bacteremic pneumonia and empyema thoracis by next-generation sequencing: a case report

Frontiers in Medicine, 2023
Nocardia species do not replicate as rapidly as other pyogenic bacteria and nocardial infections can be highly fatal, particularly in immunocompromised patients.
Fanfan Xing, Yao Xia, Qianyun Lu, Simon K. F. Lo, Susanna K. P. Lau, Patrick C. Y. Woo, Patrick C. Y. Woo, Patrick C. Y. Woo   +7 more
doaj   +1 more source