Results 11 to 20 of about 23,440 (247)

Application of Oxford Nanopore Technology to Plant Virus Detection [PDF]

Viruses, 2021
The adoption of Oxford Nanopore Technologies (ONT) sequencing as a tool in plant virology has been relatively slow despite its promise in more recent years to yield large quantities of long nucleotide sequences in real time without the need for prior amplification.
Lia W. Liefting, David W. Waite, Jeremy R. Thompson   +2 more
openaire   +3 more sources

Oxford Nanopore Technology and its Application in Liquid Biopsies

Current Genomics, 2023
Abstract: Advanced medical technologies are transforming the future of healthcare, in particular, the screening and detection of molecular-genetic changes in patients suspected of having a neoplasm. They are based on the assumption that neoplasms release small amounts of various neoplasm- specific molecules, such as tumor DNA, called circulating DNA ...
Levkova, Mariya, Chervenkov, Trifon, Angelova, Lyudmila, Dzenkov, Deyan   +3 more
openaire   +2 more sources

Oxford Nanopore Technologies [ONT] Sequencing: Clinical Validation in Genetically Heterogeneous Disorders. [PDF]

Genes (Basel)
Background/Objectives: Short-read-sequencing (SRS) is currently the standard for genetic testing in inherited human diseases. Intrinsic limitations include PCR dependency, restricted read length, and challenges in identifying structural variants (SVs), copy number variations (CNVs), and intronic small variants (SNVs/indels).
Urtis M, Paganini C, Vilardo V, Tescari A, Minetto S, Cavaliere C, Pilotto A, Giorgianni C, Cattaneo A, Tagliani M, Grasso M, Smirnova A, Ebadi P, Barzon V, Favalli V, Bimbocci A, Baragli M, Magi A, Renieri A, Arbustini E.   +19 more
europepmc   +5 more sources

Optimizing experimental design for genome sequencing and assembly with Oxford Nanopore Technologies [PDF]

Gigabyte, 2020
Abstract Background High quality reference genome sequences are the core of modern genomics. Oxford Nanopore Technologies (ONT) produces inexpensive DNA sequences in excess of 100,000 nucleotides but high error rates make sequence assembly and analysis a non-trivial problem as ...
John M. Sutton, Joshua D. Millwood, A. Case McCormack, Janna L. Fierst   +3 more
openaire   +4 more sources

Oxford Nanopore and Bionano Genomics technologies evaluation for plant structural variation detection [PDF]

BMC Genomics, 2021
Abstract Background Structural Variations (SVs) are very diverse genomic rearrangements. In the past, their detection was restricted to cytological approaches, then to NGS read size and partitionned assemblies.
Canaguier, Aurélie, Guilbaud, Romane, Denis, Erwan, Magdelenat, Ghislaine, Belser, Caroline, Istace, Benjamin, Cruaud, Corinne, Wincker, Patrick, Le Paslier, Marie-Christine, Faivre-Rampant, Patricia, Barbe, Valérie   +10 more
openaire   +5 more sources

Applications and potentials of nanopore sequencing in the (epi)genome and (epi)transcriptome era

The Innovation, 2021
Summary: The Human Genome Project opened an era of (epi)genomic research, and also provided a platform for the development of new sequencing technologies.
Shangqian Xie, Amy Wing-Sze Leung, Zhenxian Zheng, Dake Zhang, Chuanle Xiao, Ruibang Luo, Ming Luo, Shoudong Zhang   +7 more
doaj   +1 more source

pycoMeth: a toolbox for differential methylation testing from Nanopore methylation calls

Genome Biology, 2023
We present pycoMeth, a toolbox to store, manage and analyze DNA methylation calls from long-read sequencing data obtained using the Oxford Nanopore Technologies sequencing platform.
Rene Snajder, Adrien Leger, Oliver Stegle, Marc Jan Bonder   +3 more
doaj   +1 more source

Nanopore sequencing technology and its application in plant virus diagnostics

Frontiers in Microbiology, 2022
Plant viruses threaten crop yield and quality; thus, efficient and accurate pathogen diagnostics are critical for crop disease management and control. Recent advances in sequencing technology have revolutionized plant virus research.
Kai Sun, Yi Liu, Xin Zhou, Chuanlin Yin, Pengjun Zhang, Qianqian Yang, Lingfeng Mao, Xuping Shentu, Xiaoping Yu   +8 more
doaj   +1 more source

Nanopore sequencing-based genome assembly and evolutionary genomics of circum-basmati rice

Genome Biology, 2020
Background The circum-basmati group of cultivated Asian rice (Oryza sativa) contains many iconic varieties and is widespread in the Indian subcontinent.
Jae Young Choi, Zoe N. Lye, Simon C. Groen, Xiaoguang Dai, Priyesh Rughani, Sophie Zaaijer, Eoghan D. Harrington, Sissel Juul, Michael D. Purugganan   +8 more
doaj   +1 more source

Sequencing and Chromosome-Scale Assembly of Plant Genomes, Brassica rapa as a Use Case

Biology, 2021
With the rise of long-read sequencers and long-range technologies, delivering high-quality plant genome assemblies is no longer reserved to large consortia.
Benjamin Istace, Caroline Belser, Cyril Falentin, Karine Labadie, Franz Boideau, Gwenaëlle Deniot, Loeiz Maillet, Corinne Cruaud, Laurie Bertrand, Anne-Marie Chèvre, Patrick Wincker, Mathieu Rousseau-Gueutin, Jean-Marc Aury   +12 more
doaj   +1 more source