Results 21 to 30 of about 23,440 (247)

Profiling of Human Gut Virome with Oxford Nanopore Technology

Medicine in Microecology, 2020
Abstract Human gut virome play critical roles in maintaining gut microbial composition and functionality, as well as host physiology and immunology. Yet, there are insufficient amount of studies on this topic mainly due to methodological limitations, including enrichment of viruses (phages and host viruses) as well as short read-length ...
Cao, Jiabao, Zhang, Yuqing, Dai, Min, Xu, Jiayue, Chen, Liang, Zhang, Faming, Zhao, Na, Wang, Jun   +7 more
openaire   +2 more sources

Complete sequence verification of plasmid DNA using the Oxford Nanopore Technologies' MinION device. [PDF]

BMC Bioinformatics, 2023
Abstract Background Sequence verification is essential for plasmids used as critical reagents or therapeutic products. Typically, high-quality plasmid sequence is achieved through capillary-based Sanger sequencing, requiring customized sets of primers for each plasmid.
Brown SD, Dreolini L, Wilson JF, Balasundaram M, Holt RA.   +4 more
europepmc   +4 more sources

BoardION: real-time monitoring of Oxford Nanopore Technologies devices [PDF]

, 2020
Abstract Summary One of the main advantages of the Oxford Nanopore Technology (ONT) is the possibility of sequencing in real time. However, the ONT sequencing interface is not sufficient to explore the quality of sequencing data in depth and existing tools do not take full advantage of real-time data
Aimeric Bruno, Jean-Marc Aury, Stefan Engelen   +2 more
openaire   +1 more source

Amplicon sequencing with Oxford nanopore technologies as a diagnostic alternative for small ruminant lentiviruses in sheep. [PDF]

Sci Rep
Abstract In Europe, Maedi-Visna disease has high prevalence rates at the individual and flock levels, respectively, and is regarded as one of the most significant infectious disease in sheep. The lack of treatment or a commercial vaccine underscores the need for accurate and reliable diagnostic tools to support ...
Serrano M, González C, Roy R, Fernández A, Arranz JJ, Calvo JH, Jiménez MÁ, Puente-Sánchez F.   +7 more
europepmc   +4 more sources

Benchmarking Oxford Nanopore read alignment‐based insertion and deletion detection in crop plant genomes

The Plant Genome, 2023
Structural variations (SVs) are larger polymorphisms (> 50 bp in length), which consist of insertions, deletions, inversions, duplications, and translocations.
Gözde Yildiz, Silvia F. Zanini, Nazanin P. Afsharyan, Christian Obermeier, Rod J. Snowdon, Agnieszka A. Golicz   +5 more
doaj   +1 more source

An Introduction to Nanopore Sequencing: Past, Present, and Future Considerations

Micromachines, 2023
There has been significant progress made in the field of nanopore biosensor development and sequencing applications, which address previous limitations that restricted widespread nanopore use.
Morgan MacKenzie, Christos Argyropoulos
doaj   +1 more source

Constructing a Reference Genome in a Single Lab: The Possibility to Use Oxford Nanopore Technology [PDF]

Plants, 2019
The whole genome sequencing (WGS) has become a crucial tool in understanding genome structure and genetic variation. The MinION sequencing of Oxford Nanopore Technologies (ONT) is an excellent approach for performing WGS and it has advantages in comparison with other Next-Generation Sequencing (NGS): It is relatively inexpensive, portable, has simple ...
Yun Gyeong Lee, Sang Chul Choi, Yuna Kang, Kyeong Min Kim, Chon-Sik Kang, Changsoo Kim   +5 more
openaire   +3 more sources

Identification of an emerging cucumber virus in Taiwan using Oxford nanopore sequencing technology

Plant Methods, 2022
Abstract Background In June 2020, severe symptoms of leaf mosaic and fruit malformation were observed on greenhouse-grown cucumber plants in Xizhou Township of Changhua County, Taiwan. An unknown virus, designated CX-2, was isolated from a diseased cucumber sample by single lesion isolation on Chenopodium quinoa leaves ...
Zi-Xuan Dong, Chian-Chi Lin, Yuh-Kun Chen, Chia-Cheng Chou, Tsung-Chi Chen   +4 more
openaire   +3 more sources

Accurate profiling of forensic autosomal STRs using the Oxford Nanopore Technologies MinION device [PDF]

Forensic Science International: Genetics, 2021
Abstract The high variability characteristic of short tandem repeat (STR) markers is harnessed for human identification in forensic genetic analyses. Despite the power and reliability of current typing techniques, sequence-level information both within and around STRs are masked in the length-based profiles generated.
Courtney L. Hall, Rupesh K. Kesharwani, Nicole R. Phillips, John V. Planz, Fritz J. Sedlazeck, Roxanne R. Zascavage   +5 more
openaire   +3 more sources

Crystal structure of an invertebrate cytolysin pore reveals unique properties and mechanism of assembly

Nature Communications, 2016
Pore-forming toxins act by forming oligomeric pores in lipid membranes. Here the authors report the crystal structure of the lysenin pore, providing insights into the assembly and function of the pore in addition to suggesting that its properties make ...
Marjetka Podobnik, Peter Savory, Nejc Rojko, Matic Kisovec, Neil Wood, Richard Hambley, Jonathan Pugh, E. Jayne Wallace, Luke McNeill, Mark Bruce, Idlir Liko, Timothy M. Allison, Shahid Mehmood, Neval Yilmaz, Toshihide Kobayashi, Robert J. C. Gilbert, Carol V. Robinson, Lakmal Jayasinghe, Gregor Anderluh   +18 more
doaj   +1 more source