Results 21 to 30 of about 599,955 (287)

PANDAseq: paired-end assembler for illumina sequences [PDF]

BMC Bioinformatics, 2012
Abstract Background Illumina paired-end reads are used to analyse microbial communities by targeting amplicons of the 16S rRNA gene. Publicly available tools are needed to assemble overlapping paired-end reads while correcting mismatches and uncalled bases; many errors could be corrected to obtain higher sequence ...
Masella Andre P, Bartram Andrea K, Truszkowski Jakub M, Brown Daniel G, Neufeld Josh D   +4 more
openaire   +3 more sources

Illumina sequencing of 15 deafness genes using fragmented amplicons [PDF]

, 2014
BACKGROUND: Resequencing of deafness related genes using GS FLX massive parallel sequencing of PCR amplicons spanning selected genes has previously been reported as a successful strategy to discover causal variants.
Coucke, Paul, De Keulenaer, Sarah, De Schrijver, Joachim, Deforce, Dieter, Van Criekinge, Wim, Van Nieuwerburgh, Filip, Vandesompele, Jo   +6 more
core   +1 more source

Development and performance of a targeted whole exome sequencing enrichment kit for the dog (Canis Familiaris Build 3.1) [PDF]

, 2014
Whole exome sequencing is a technique that aims to selectively sequence all exons of protein-coding genes. A canine whole exome sequencing enrichment kit was designed based on the latest canine reference genome (build 3.1.72).
Bavegems, Valérie, Broeckx, Bart, Coopman, Frank, De Keulenaer, Sarah, De Meester, Ellen, Deforce, Dieter, Van Nieuwerburgh, Filip, Verhoeven, Geert   +7 more
core   +2 more sources

Comparison of Illumina and Oxford Nanopore Technology for genome analysis of Francisella tularensis, Bacillus anthracis, and Brucella suis

BMC Genomics, 2023
Background Bacterial epidemiology needs to understand the spread and dissemination of strains in a One Health context. This is important for highly pathogenic bacteria such as Bacillus anthracis, Brucella species, and Francisella tularensis. Whole genome
Jörg Linde, Hanka Brangsch, Martin Hölzer, Christine Thomas, Mandy C. Elschner, Falk Melzer, Herbert Tomaso   +6 more
doaj   +1 more source

BFC: correcting Illumina sequencing errors [PDF]

Bioinformatics, 2015
Abstract Summary: BFC is a free, fast and easy-to-use sequencing error corrector designed for Illumina short reads. It uses a non-greedy algorithm but still maintains a speed comparable to implementations based on greedy methods. In evaluations on real data, BFC appears to correct more errors with fewer overcorrections in comparison to ...
openaire   +2 more sources

nifH amplification for Illumina sequencing v1 [PDF]

, 2020
For metabarcoding purpose, the first step involves the amplification by PCR of a given gene region (for example V4 or V9 region of 18S rRNA gene) or gene itself if its size does not exceed 600bp (the longest fragment size that can be sequenced by Illumina technology).
Estelle Bigeard, Adriana Lopes Dos Santos, Catherine Ribeiro   +2 more
openaire   +1 more source

Illumina Sequencing Library Construction from ChIP DNA

Bio-Protocol, 2012
The Illumina sequencing platform is very popular among next-generation sequencing platforms. However, the DNA sequencing library construction kit provided by Illumina is considerably expensive.
Wei Zheng
doaj   +1 more source

High-quality single amplicon sequencing method for illumina MiSeq platform using pool of ‘N’ (0–10) spacer-linked target specific primers without PhiX spike-in

BMC Genomics, 2023
Background Illumina sequencing platform requires base diversity in the initial 11 cycles for efficient cluster identification and colour matrix estimation. This limitation yields low-quality data for amplicon libraries having homogeneous base composition.
Tejali Naik, Mohak Sharda, Lakshminarayanan C P, Kumar Virbhadra, Awadhesh Pandit   +4 more
doaj   +1 more source

Paragraph: a graph-based structural variant genotyper for short-read sequence data

Genome Biology, 2019
Accurate detection and genotyping of structural variations (SVs) from short-read data is a long-standing area of development in genomics research and clinical sequencing pipelines.
Sai Chen, Peter Krusche, Egor Dolzhenko, Rachel M. Sherman, Roman Petrovski, Felix Schlesinger, Melanie Kirsche, David R. Bentley, Michael C. Schatz, Fritz J. Sedlazeck, Michael A. Eberle   +10 more
doaj   +1 more source

Evaluation of the MGISEQ-2000 Sequencing Platform for Illumina Target Capture Sequencing Libraries

Frontiers in Genetics, 2021
Illumina is the leading sequencing platform in the next-generation sequencing (NGS) market globally. In recent years, MGI Tech has presented a series of new sequencers, including DNBSEQ-T7, MGISEQ-2000 and MGISEQ-200.
Jidong Lang, Jidong Lang, Jidong Lang, Rongrong Zhu, Xue Sun, Siyu Zhu, Tianbao Li, Tianbao Li, Xiaoli Shi, Yanqi Sun, Zhou Yang, Weiwei Wang, Weiwei Wang, Pingping Bing, Binsheng He, Geng Tian, Geng Tian   +16 more
doaj   +1 more source