Results 1 to 10 of about 11,169 (113)

Plant Sequence Capture Optimised for Illumina Sequencing

Bio-Protocol, 2014
Plant Sequence Capture is used for targeted resequencing of whole exomes (all exons of a genome) of complex genomes e.g. barley and its relatives (Mascher et al., 2013).
Axel Himmelbach, Manuela Knauft , Nils Stein   +2 more
doaj   +2 more sources

Evaluation of the MGISEQ-2000 Sequencing Platform for Illumina Target Capture Sequencing Libraries

Frontiers in Genetics, 2021
Illumina is the leading sequencing platform in the next-generation sequencing (NGS) market globally. In recent years, MGI Tech has presented a series of new sequencers, including DNBSEQ-T7, MGISEQ-2000 and MGISEQ-200.
Jidong Lang, Jidong Lang, Jidong Lang, Rongrong Zhu, Xue Sun, Siyu Zhu, Tianbao Li, Tianbao Li, Xiaoli Shi, Yanqi Sun, Zhou Yang, Weiwei Wang, Weiwei Wang, Pingping Bing, Binsheng He, Geng Tian, Geng Tian   +16 more
doaj   +3 more sources

Breaking the Standard: Can Oxford Nanopore Technologies Sequencing Compete With Illumina in Protistan Amplicon Studies?

Environmental DNA
Advancements in sequencing technologies have revolutionized environmental research, leading to a superior understanding of ecosystem functions. In recent years, high‐throughput sequencing techniques evolved from short‐read Illumina sequencing to long ...
Dana Bludau, Guido Sieber, Manan Shah, Aman Deep, Jens Boenigk, Daniela Beisser   +5 more
doaj   +2 more sources

Viral Metagenomics: Analysis of Begomoviruses by Illumina High-Throughput Sequencing

Viruses, 2014
Traditional DNA sequencing methods are inefficient, lack the ability to discern the least abundant viral sequences, and ineffective for determining the extent of variability in viral populations. Here, populations of single-stranded DNA plant begomoviral
Ali Idris, Mohammed Al-Saleh, Marek J. Piatek, Ibrahim Al-Shahwan, Shahjahan Ali, Judith K. Brown   +5 more
doaj   +3 more sources

Improved Protocols for Illumina Sequencing [PDF]

Current Protocols in Human Genetics, 2013
AbstractIn this unit, we describe a set of improvements that have been made to the standard Illumina protocols to make the sequencing process more reliable in a high‐throughput environment, reduce amplification bias, narrow the distribution of insert sizes, and reliably obtain high yields of data. Curr. Protoc. Hum. Genet. 79:18.2.1‐18.2.42.
Iraad F, Bronner, Michael A, Quail, Daniel J, Turner, Harold, Swerdlow   +3 more
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Sequencing error profiles of Illumina sequencing instruments [PDF]

NAR Genomics and Bioinformatics, 2021
Abstract Sequencing technology has achieved great advances in the past decade. Studies have previously shown the quality of specific instruments in controlled conditions. Here, we developed a method able to retroactively determine the error rate of most public sequencing datasets.
Nicholas Stoler, Anton Nekrutenko
openaire   +2 more sources

A simple, single-tube overlapping amplicon-targeted Illumina sequencing assay.

PLoS ONE, 2023
Targeted amplicon sequencing to identify pathogens, resistance-conferring mutations, and strain types is an important tool in diagnosing and treating infections. However, due to the short read limitations of Illumina sequencing, many applications require
Jason D Limberis, Alina Nalyvayko, Joel D Ernst, John Z Metcalfe   +3 more
doaj   +3 more sources

Structural variant analysis of a cancer reference cell line sample using multiple sequencing technologies

Genome Biology, 2022
Background The cancer genome is commonly altered with thousands of structural rearrangements including insertions, deletions, translocation, inversions, duplications, and copy number variations.
Keyur Talsania, Tsai-wei Shen, Xiongfong Chen, Erich Jaeger, Zhipan Li, Zhong Chen, Wanqiu Chen, Bao Tran, Rebecca Kusko, Limin Wang, Andy Wing Chun Pang, Zhaowei Yang, Sulbha Choudhari, Michael Colgan, Li Tai Fang, Andrew Carroll, Jyoti Shetty, Yuliya Kriga, Oksana German, Tatyana Smirnova, Tiantain Liu, Jing Li, Ben Kellman, Karl Hong, Alex R. Hastie, Aparna Natarajan, Ali Moshrefi, Anastasiya Granat, Tiffany Truong, Robin Bombardi, Veronnica Mankinen, Daoud Meerzaman, Christopher E. Mason, Jack Collins, Eric Stahlberg, Chunlin Xiao, Charles Wang, Wenming Xiao, Yongmei Zhao   +38 more
doaj   +1 more source

Best practices for the interpretation and reporting of clinical whole genome sequencing

npj Genomic Medicine, 2022
Whole genome sequencing (WGS) shows promise as a first-tier diagnostic test for patients with rare genetic disorders. However, standards addressing the definition and deployment practice of a best-in-class test are lacking.
Christina A. Austin-Tse, Vaidehi Jobanputra, Denise L. Perry, David Bick, Ryan J. Taft, Eric Venner, Richard A. Gibbs, Ted Young, Sarah Barnett, John W. Belmont, Nicole Boczek, Shimul Chowdhury, Katarzyna A. Ellsworth, Saurav Guha, Shashikant Kulkarni, Cherisse Marcou, Linyan Meng, David R. Murdock, Atteeq U. Rehman, Elizabeth Spiteri, Amanda Thomas-Wilson, Hutton M. Kearney, Heidi L. Rehm, Medical Genome Initiative*   +23 more
doaj   +1 more source

Sequence-specific error profile of Illumina sequencers [PDF]

Nucleic Acids Research, 2011
We identified the sequence-specific starting positions of consecutive miscalls in the mapping of reads obtained from the Illumina Genome Analyser (GA). Detailed analysis of the miscall pattern indicated that the underlying mechanism involves sequence-specific interference of the base elongation process during sequencing. The two major sequence patterns
Nakamura, Kensuke, Oshima, Taku, Morimoto, Takuya, Ikeda, Shun, Yoshikawa, Hirofumi, Shiwa, Yuh, Ishikawa, Shu, Linak, Margaret C., Hirai, Aki, Takahashi, Hiroki, Altaf-Ul-Amin, Md., Ogasawara, Naotake, Kanaya, Shigehiko   +12 more
openaire   +2 more sources