Results 1 to 10 of about 463,626 (303)

A simple, single-tube overlapping amplicon-targeted Illumina sequencing assay. [PDF]

PLoS ONE, 2023
Targeted amplicon sequencing to identify pathogens, resistance-conferring mutations, and strain types is an important tool in diagnosing and treating infections. However, due to the short read limitations of Illumina sequencing, many applications require
Jason D Limberis, Alina Nalyvayko, Joel D Ernst, John Z Metcalfe   +3 more
doaj   +7 more sources

Closing the gap: Oxford Nanopore Technologies R10 sequencing allows comparable results to Illumina sequencing for SNP-based outbreak investigation of bacterial pathogens [PDF]

Journal of Clinical Microbiology
Whole-genome sequencing has become the method of choice for bacterial outbreak investigation, with most clinical and public health laboratories currently routinely using short-read Illumina sequencing.
Bert Bogaerts, Kevin Vanneste
exaly   +4 more sources

From reads to results: comparing Oxford Nanopore to Illumina sequencing for citrus virus surveillance [PDF]

BMC Genomics
Background ONT sequencing has been previously evaluated for its ability to detect plant viruses and viroids. Its advantages, such as longer read lengths and real-time analysis, compete with extensively validated Illumina platforms for possible ...
Madelein Dippenaar, Hans Jacob Maree, Rachelle Bester   +2 more
doaj   +2 more sources

Direct comparison of clear DX Nanopore and Illumina sequencing of SARS-CoV-2 [PDF]

Microbiology Spectrum
There are limited data directly comparing SARS-CoV-2 sequencing methods using two major commercial approaches, Oxford Nanopore Technologies Clear DX (ONTDX) and Illumina.
Wesley Hottel, Valerie Reeb, Erik Twait, Kristen Zanon, Munok Hwang, Hosoon Choi, Piyali Chatterjee, Jinhua Xiang, Jeffery Meier, Michael Pentella, Eric McIndoo, Mary Cloud B. Ammons, Chetan Jinadatha, Jack T. Stapleton   +13 more
doaj   +2 more sources

Evaluation of the MGISEQ-2000 Sequencing Platform for Illumina Target Capture Sequencing Libraries

Frontiers in Genetics, 2021
Illumina is the leading sequencing platform in the next-generation sequencing (NGS) market globally. In recent years, MGI Tech has presented a series of new sequencers, including DNBSEQ-T7, MGISEQ-2000 and MGISEQ-200.
Jidong Lang, Jidong Lang, Jidong Lang, Rongrong Zhu, Xue Sun, Siyu Zhu, Tianbao Li, Tianbao Li, Xiaoli Shi, Yanqi Sun, Zhou Yang, Weiwei Wang, Weiwei Wang, Pingping Bing, Binsheng He, Geng Tian, Geng Tian   +16 more
doaj   +3 more sources

Systematic Comparison of Nanopore and Illumina Sequencing for the Detection of Plant Viruses and Viroids Using Total RNA Sequencing Approach [PDF]

Frontiers in Microbiology, 2022
High-throughput sequencing (HTS) has become an important tool for plant virus detection and discovery. Nanopore sequencing has been rapidly developing in the recent years and offers new possibilities for fast diagnostic applications of HTS.
Anja Pecman, Anja Pecman, Ian Adams, Ion Gutiérrez-Aguirre, Adrian Fox, Neil Boonham, Maja Ravnikar, Denis Kutnjak   +7 more
doaj   +2 more sources

Breaking the Standard: Can Oxford Nanopore Technologies Sequencing Compete With Illumina in Protistan Amplicon Studies?

Environmental DNA
Advancements in sequencing technologies have revolutionized environmental research, leading to a superior understanding of ecosystem functions. In recent years, high‐throughput sequencing techniques evolved from short‐read Illumina sequencing to long ...
Dana Bludau, Guido Sieber, Manan Shah, Aman Deep, Jens Boenigk, Daniela Beisser   +5 more
doaj   +2 more sources

Viral Metagenomics: Analysis of Begomoviruses by Illumina High-Throughput Sequencing

Viruses, 2014
Traditional DNA sequencing methods are inefficient, lack the ability to discern the least abundant viral sequences, and ineffective for determining the extent of variability in viral populations. Here, populations of single-stranded DNA plant begomoviral
Ali Idris, Mohammed Al-Saleh, Marek J. Piatek, Ibrahim Al-Shahwan, Shahjahan Ali, Judith K. Brown   +5 more
doaj   +3 more sources

Structural variant analysis of a cancer reference cell line sample using multiple sequencing technologies

Genome Biology, 2022
Background The cancer genome is commonly altered with thousands of structural rearrangements including insertions, deletions, translocation, inversions, duplications, and copy number variations.
Keyur Talsania, Tsai-wei Shen, Xiongfong Chen, Erich Jaeger, Zhipan Li, Zhong Chen, Wanqiu Chen, Bao Tran, Rebecca Kusko, Limin Wang, Andy Wing Chun Pang, Zhaowei Yang, Sulbha Choudhari, Michael Colgan, Li Tai Fang, Andrew Carroll, Jyoti Shetty, Yuliya Kriga, Oksana German, Tatyana Smirnova, Tiantain Liu, Jing Li, Ben Kellman, Karl Hong, Alex R. Hastie, Aparna Natarajan, Ali Moshrefi, Anastasiya Granat, Tiffany Truong, Robin Bombardi, Veronnica Mankinen, Daoud Meerzaman, Christopher E. Mason, Jack Collins, Eric Stahlberg, Chunlin Xiao, Charles Wang, Wenming Xiao, Yongmei Zhao   +38 more
doaj   +1 more source

Best practices for the interpretation and reporting of clinical whole genome sequencing

npj Genomic Medicine, 2022
Whole genome sequencing (WGS) shows promise as a first-tier diagnostic test for patients with rare genetic disorders. However, standards addressing the definition and deployment practice of a best-in-class test are lacking.
Christina A. Austin-Tse, Vaidehi Jobanputra, Denise L. Perry, David Bick, Ryan J. Taft, Eric Venner, Richard A. Gibbs, Ted Young, Sarah Barnett, John W. Belmont, Nicole Boczek, Shimul Chowdhury, Katarzyna A. Ellsworth, Saurav Guha, Shashikant Kulkarni, Cherisse Marcou, Linyan Meng, David R. Murdock, Atteeq U. Rehman, Elizabeth Spiteri, Amanda Thomas-Wilson, Hutton M. Kearney, Heidi L. Rehm, Medical Genome Initiative*   +23 more
doaj   +1 more source