A simple, single-tube overlapping amplicon-targeted Illumina sequencing assay. [PDF]
PLoS ONE, 2023 Targeted amplicon sequencing to identify pathogens, resistance-conferring mutations, and strain types is an important tool in diagnosing and treating infections. However, due to the short read limitations of Illumina sequencing, many applications require
Jason D Limberis +3 more
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Closing the gap: Oxford Nanopore Technologies R10 sequencing allows comparable results to Illumina sequencing for SNP-based outbreak investigation of bacterial pathogens. [PDF]
Journal of Clinical MicrobiologyWhole-genome sequencing has become the method of choice for bacterial outbreak investigation, with most clinical and public health laboratories currently routinely using short-read Illumina sequencing.
Bert Bogaerts, Kevin Vanneste
exaly +3 more sources
From reads to results: comparing Oxford Nanopore to Illumina sequencing for citrus virus surveillance [PDF]
BMC GenomicsBackground ONT sequencing has been previously evaluated for its ability to detect plant viruses and viroids. Its advantages, such as longer read lengths and real-time analysis, compete with extensively validated Illumina platforms for possible ...
Madelein Dippenaar +2 more
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Direct comparison of clear DX Nanopore and Illumina sequencing of SARS-CoV-2 [PDF]
Microbiology SpectrumThere are limited data directly comparing SARS-CoV-2 sequencing methods using two major commercial approaches, Oxford Nanopore Technologies Clear DX (ONTDX) and Illumina.
Wesley Hottel +13 more
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Systematic Comparison of Nanopore and Illumina Sequencing for the Detection of Plant Viruses and Viroids Using Total RNA Sequencing Approach [PDF]
Frontiers in Microbiology, 2022 High-throughput sequencing (HTS) has become an important tool for plant virus detection and discovery. Nanopore sequencing has been rapidly developing in the recent years and offers new possibilities for fast diagnostic applications of HTS.
Anja Pecman +7 more
doaj +2 more sources
Environmental DNAAdvancements in sequencing technologies have revolutionized environmental research, leading to a superior understanding of ecosystem functions. In recent years, high‐throughput sequencing techniques evolved from short‐read Illumina sequencing to long ...
Jens Boenigk, Manan Shah, Aman Deep
exaly +2 more sources
Viral Metagenomics: Analysis of Begomoviruses by Illumina High-Throughput Sequencing
Viruses, 2014 Traditional DNA sequencing methods are inefficient, lack the ability to discern the least abundant viral sequences, and ineffective for determining the extent of variability in viral populations. Here, populations of single-stranded DNA plant begomoviral
Shahjahan Ali +2 more
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Genome Biology, 2022 Background The cancer genome is commonly altered with thousands of structural rearrangements including insertions, deletions, translocation, inversions, duplications, and copy number variations.
Keyur Talsania +38 more
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Best practices for the interpretation and reporting of clinical whole genome sequencing
npj Genomic Medicine, 2022 Whole genome sequencing (WGS) shows promise as a first-tier diagnostic test for patients with rare genetic disorders. However, standards addressing the definition and deployment practice of a best-in-class test are lacking.
Christina A. Austin-Tse +23 more
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Comparison of illumina and 454 deep sequencing in participants failing raltegravir-based antiretroviral therapy. [PDF]
PLoS ONE, 2014 The impact of raltegravir-resistant HIV-1 minority variants (MVs) on raltegravir treatment failure is unknown. Illumina sequencing offers greater throughput than 454, but sequence analysis tools for viral sequencing are needed.
Jonathan Z Li +30 more
doaj +1 more source