Results 11 to 20 of about 463,626 (303)
Plant Sequence Capture Optimised for Illumina Sequencing
Bio-Protocol, 2014 Plant Sequence Capture is used for targeted resequencing of whole exomes (all exons of a genome) of complex genomes e.g. barley and its relatives (Mascher et al., 2013).
Axel Himmelbach +2 more
doaj +2 more sources
Illumina Sequencing of Bisulfite-Converted DNA Libraries [PDF]
Cold Spring Harbor Protocols, 2016 Here we describe a standard MethylC-seq protocol using single-read sequencing on an Illumina Genome Analyzer II platform. The protocol involves ligation of methylated sequencing adaptors to sonicated genomic DNA, gel purification, sodium bisulfite conversion, polymerase chain reaction (PCR) amplification, and sequencing.
Paul M, Lizardi +2 more
openaire +3 more sources
Sequencing error profiles of Illumina sequencing instruments. [PDF]
NAR Genom Bioinform, 2021 Abstract Sequencing technology has achieved great advances in the past decade. Studies have previously shown the quality of specific instruments in controlled conditions. Here, we developed a method able to retroactively determine the error rate of most public sequencing datasets.
Stoler N, Nekrutenko A.
europepmc +4 more sources
Improved Protocols for Illumina Sequencing [PDF]
Current Protocols in Human Genetics, 2013 AbstractIn this unit, we describe a set of improvements that have been made to the standard Illumina protocols to make the sequencing process more reliable in a high‐throughput environment, reduce amplification bias, narrow the distribution of insert sizes, and reliably obtain high yields of data. Curr. Protoc. Hum. Genet. 79:18.2.1‐18.2.42.
Iraad F, Bronner +3 more
+6 more sources
Sequence-specific error profile of Illumina sequencers [PDF]
Nucleic Acids Research, 2011 We identified the sequence-specific starting positions of consecutive miscalls in the mapping of reads obtained from the Illumina Genome Analyser (GA). Detailed analysis of the miscall pattern indicated that the underlying mechanism involves sequence-specific interference of the base elongation process during sequencing. The two major sequence patterns
Nakamura, Kensuke +12 more
openaire +2 more sources
Review on Illumina Sequencing Technology
Austin Journal of Veterinary Science & Animal Husbandry, 2022 Illumina sequencing process utilizes biochemical methods to determine the correct order of nucleotide bases in a deoxyribonucleic acid. Macromolecule using sequencing-by-synthesis and reversible dye-terminators that enable the identification of single bases are introduced into DNA strands and used to determine the series of base pairs in DNA.
Emiyu K, Lelisa K
openaire +1 more source
Illumina sequencing results. [PDF]
, 2022 Illumina sequencing results.
Ashley M. Reaume (8691987) +3 more
core +1 more source
Comparison of illumina and 454 deep sequencing in participants failing raltegravir-based antiretroviral therapy. [PDF]
PLoS ONE, 2014 The impact of raltegravir-resistant HIV-1 minority variants (MVs) on raltegravir treatment failure is unknown. Illumina sequencing offers greater throughput than 454, but sequence analysis tools for viral sequencing are needed.
Jonathan Z Li +30 more
doaj +1 more source
Monitoring Error Rates In Illumina Sequencing [PDF]
Journal of Biomolecular Techniques : JBT, 2016 Guaranteeing high-quality next-generation sequencing data in a rapidly changing environment is an ongoing challenge. The introduction of the Illumina NextSeq 500 and the depreciation of specific metrics from Illumina's Sequencing Analysis Viewer (SAV; Illumina, San Diego, CA, USA) have made it more difficult to determine directly the baseline error ...
Leigh J, Manley +2 more
openaire +2 more sources
ReSeq simulates realistic Illumina high-throughput sequencing data
Genome Biology, 2021 In high-throughput sequencing data, performance comparisons between computational tools are essential for making informed decisions at each step of a project.
Stephan Schmeing, Mark D. Robinson
doaj +1 more source