Monitoring Error Rates In Illumina Sequencing [PDF]
Journal of Biomolecular Techniques : JBT, 2016 Guaranteeing high-quality next-generation sequencing data in a rapidly changing environment is an ongoing challenge. The introduction of the Illumina NextSeq 500 and the depreciation of specific metrics from Illumina's Sequencing Analysis Viewer (SAV ...
Leigh J. Manley +2 more
semanticscholar +5 more sources
Evaluation of the MGISEQ-2000 Sequencing Platform for Illumina Target Capture Sequencing Libraries [PDF]
Frontiers in Genetics, 2021 Illumina is the leading sequencing platform in the next-generation sequencing (NGS) market globally. In recent years, MGI Tech has presented a series of new sequencers, including DNBSEQ-T7, MGISEQ-2000 and MGISEQ-200.
Siyu Zhu, Tianbao Li, Pingping Bing
exaly +6 more sources
A Comparison of Base-calling Algorithms for Illumina Sequencing Technology [PDF]
Briefings in Bioinformatics, 2015 Recent advances in next-generation sequencing technology have yielded increasing cost-effectiveness and higher throughput produced per run, in turn, greatly influencing the analysis of DNA sequences. Among the various sequencing technologies, Illumina is
Ashley Cacho +3 more
semanticscholar +4 more sources
Plant Sequence Capture Optimised for Illumina Sequencing
Bio-Protocol, 2014 Plant Sequence Capture is used for targeted resequencing of whole exomes (all exons of a genome) of complex genomes e.g. barley and its relatives (Mascher et al., 2013).
Axel Himmelbach +2 more
doaj +3 more sources
Analysis, Optimization and Verification of Illumina-Generated 16S rRNA Gene Amplicon Surveys [PDF]
PLoS ONE, 2014 The exploration of microbial communities by sequencing 16S rRNA genes has expanded with low-cost, high-throughput sequencing instruments. Illumina-based 16S rRNA gene sequencing has recently gained popularity over 454 pyrosequencing due to its lower ...
Michael C Nelson +2 more
exaly +2 more sources
ReSeq simulates realistic Illumina high-throughput sequencing data [PDF]
Genome Biology, 2021 In high-throughput sequencing data, performance comparisons between computational tools are essential for making informed decisions at each step of a project.
Stephan Schmeing, Mark D. Robinson
doaj +6 more sources
Shotgun metagenome data of a defined mock community using Oxford Nanopore, PacBio and Illumina technologies [PDF]
Scientific Data, 2019 Metagenomic sequence data from defined mock communities is crucial for the assessment of sequencing platform performance and downstream analyses, including assembly, binning and taxonomic assignment.
Alison E Murray +2 more
exaly +2 more sources
Development of a Dual-Index Sequencing Strategy and Curation Pipeline for Analyzing Amplicon Sequence Data on the MiSeq Illumina Sequencing Platform [PDF]
Applied and Environmental Microbiology, 2013 James J. Kozich +4 more
openalex +2 more sources
Adapterama II: universal amplicon sequencing on Illumina platforms (TaggiMatrix) [PDF]
PeerJ, 2019 Next-generation sequencing (NGS) of amplicons is used in a wide variety of contexts. In many cases, NGS amplicon sequencing remains overly expensive and inflexible, with library preparation strategies relying upon the fusion of locus-specific primers to ...
Travis C. Glenn +26 more
doaj +7 more sources
Addressing challenges in the production and analysis of illumina sequencing data [PDF]
BMC Genomics, 2011 Abstract Advances in DNA sequencing technologies have made it possible to generate large amounts of sequence data very rapidly and at substantially lower cost than capillary sequencing. These new technologies have specific characteristics and limitations that require either consideration during project design, or which must be addressed ...
Martin Kircher +2 more
openalex +7 more sources