Results 21 to 30 of about 1,311,956 (360)

Comparison of the MGISEQ-2000 and Illumina HiSeq 4000 sequencing platforms for RNA sequencing [PDF]

Genomics & Informatics, 2019
Currently, Illumina sequencers are the globally leading sequencing platform in the next-generation sequencing market. Recently, MGI Tech launched a series of new sequencers, including the MGISEQ-2000, which promise to deliver high-quality sequencing data
Sol A Jeon, Jong Lyul Park, Jong-Hwan Kim, Jeong Hwan Kim, Yong Sung Kim, Jin Cheon Kim, Seon-Young Kim   +6 more
doaj   +4 more sources

stilPCR increases the effective sequencing length of Illumina targeted next-generation sequencing.

PLoS ONE
Identifying pathogens, resistance-conferring mutations, and strain types through targeted amplicon sequencing is an important tool. However, due to the limitations of short read sequencing, many applications require the division of limited clinical ...
Jason D Limberis, Roland J Nagel, Soumitesh Chakravorty, Dena Block, Scott Dewell, Alina Nalyvayko, Zach Howard, Grant Theron, Rouxjeane Venter, John Z Metcalfe   +9 more
doaj   +5 more sources

Rapid Microsatellite Identification from Illumina Paired-End Genomic Sequencing in Two Birds and a Snake

PLoS ONE, 2012
Todd A Castoe, Diana F Tomback, Sara J Oyler-mccance   +2 more
exaly   +3 more sources

Improved Protocols for Illumina Sequencing [PDF]

Current Protocols in Human Genetics, 2013
AbstractIn this unit, we describe a set of improvements that have been made to the standard Illumina protocols to make the sequencing process more reliable in a high‐throughput environment, reduce amplification bias, narrow the distribution of insert sizes, and reliably obtain high yields of data. Curr. Protoc. Hum. Genet. 79:18.2.1‐18.2.42.
Daniel J. Turner, Iraad F. Bronner, Michael A. Quail, Harold Swerdlow   +3 more
openaire   +7 more sources

Structural variant analysis of a cancer reference cell line sample using multiple sequencing technologies

Genome Biology, 2022
Background The cancer genome is commonly altered with thousands of structural rearrangements including insertions, deletions, translocation, inversions, duplications, and copy number variations.
Keyur Talsania, Tsai-wei Shen, Xiongfong Chen, Erich Jaeger, Zhipan Li, Zhong Chen, Wanqiu Chen, Bao Tran, Rebecca Kusko, Limin Wang, Andy Wing Chun Pang, Zhaowei Yang, Sulbha Choudhari, Michael Colgan, Li Tai Fang, Andrew Carroll, Jyoti Shetty, Yuliya Kriga, Oksana German, Tatyana Smirnova, Tiantain Liu, Jing Li, Ben Kellman, Karl Hong, Alex R. Hastie, Aparna Natarajan, Ali Moshrefi, Anastasiya Granat, Tiffany Truong, Robin Bombardi, Veronnica Mankinen, Daoud Meerzaman, Christopher E. Mason, Jack Collins, Eric Stahlberg, Chunlin Xiao, Charles Wang, Wenming Xiao, Yongmei Zhao   +38 more
doaj   +1 more source

Sequence-specific error profile of Illumina sequencers [PDF]

Nucleic Acids Research, 2011
We identified the sequence-specific starting positions of consecutive miscalls in the mapping of reads obtained from the Illumina Genome Analyser (GA). Detailed analysis of the miscall pattern indicated that the underlying mechanism involves sequence-specific interference of the base elongation process during sequencing. The two major sequence patterns
Takuya Morimoto, Kensuke Nakamura, Taku Oshima, Shun Ikeda, Hiroki Takahashi, Shu Ishikawa, Yuh Shiwa, Aki Hirai, Naotake Ogasawara, Shigehiko Kanaya, Md. Altaf-Ul-Amin, Hirofumi Yoshikawa, Margaret C. Linak   +12 more
openaire   +3 more sources

Best practices for the interpretation and reporting of clinical whole genome sequencing

npj Genomic Medicine, 2022
Whole genome sequencing (WGS) shows promise as a first-tier diagnostic test for patients with rare genetic disorders. However, standards addressing the definition and deployment practice of a best-in-class test are lacking.
Christina A. Austin-Tse, Vaidehi Jobanputra, Denise L. Perry, David Bick, Ryan J. Taft, Eric Venner, Richard A. Gibbs, Ted Young, Sarah Barnett, John W. Belmont, Nicole Boczek, Shimul Chowdhury, Katarzyna A. Ellsworth, Saurav Guha, Shashikant Kulkarni, Cherisse Marcou, Linyan Meng, David R. Murdock, Atteeq U. Rehman, Elizabeth Spiteri, Amanda Thomas-Wilson, Hutton M. Kearney, Heidi L. Rehm, Medical Genome Initiative*   +23 more
doaj   +1 more source

Nanopore Is Preferable over Illumina for 16S Amplicon Sequencing of the Gut Microbiota When Species-Level Taxonomic Classification, Accurate Estimation of Richness, or Focus on Rare Taxa Is Required

Microorganisms, 2023
Nanopore sequencing is a promising technology used for 16S rRNA gene amplicon sequencing as it can provide full-length 16S reads and has a low up-front cost that allows research groups to set up their own sequencing workflows.
Márton Szoboszlay, Laetitia Schramm, David Pinzauti, J. Scerri, A. Sandionigi, Manuele Biazzo   +5 more
semanticscholar   +1 more source

Review on Illumina Sequencing Technology

Austin Journal of Veterinary Science & Animal Husbandry, 2022
Illumina sequencing process utilizes biochemical methods to determine the correct order of nucleotide bases in a deoxyribonucleic acid. Macromolecule using sequencing-by-synthesis and reversible dye-terminators that enable the identification of single ...
Emiyu K, Lelisa K
semanticscholar   +1 more source

Characterization of pseudorabies virus transcriptome by Illumina sequencing [PDF]

BMC Microbiology, 2015
Pseudorabies virus is a widely-studied model organism of the Herpesviridae family, with a compact genome arrangement of 72 known coding sequences. In order to obtain an up-to-date genetic map of the virus, a combination of RNA-sequencing approaches were applied, as recent advancements in high-throughput sequencing methods have provided a wealth of ...
Oláh, Péter, Tombácz, Dóra, Póka, Nándor, Csabai, Zsolt, Prazsák, I., Boldogkői, Zsolt   +5 more
openaire   +4 more sources