Results 31 to 40 of about 420,786 (307)

German-Wide Interlaboratory Study Compares Consistency, Accuracy and Reproducibility of Whole-Genome Short Read Sequencing

open access: yesFrontiers in Microbiology, 2020
We compared the consistency, accuracy and reproducibility of next-generation short read sequencing between ten laboratories involved in food safety (research institutes, state laboratories, universities and companies) from Germany and Austria ...
Laura Uelze   +12 more
doaj   +1 more source

Despite low viral titer in saliva samples, Sanger-based SARS-CoV-2 spike gene sequencing is highly applicable for the variant identification

open access: yesBMC Medical Genomics, 2023
Background This study aimed to compare the performance of Sanger-based SARS-CoV-2 spike gene sequencing and Next Generation Sequencing (NGS)-based full-genome sequencing for variant identification in saliva samples with low viral titer. Methods Using 241
Ko Ko   +13 more
doaj   +1 more source

Trimmomatic: a flexible trimmer for Illumina sequence data [PDF]

open access: yesBioinformatics, 2014
Abstract Motivation: Although many next-generation sequencing (NGS) read preprocessing tools already existed, we could not find any tool or combination of tools that met our requirements in terms of flexibility, correct handling of paired-end data and high performance.
Bolger, A. M., Lohse, M., Usadel, Björn
openaire   +4 more sources

Transcriptome sequencing of sesame (Sesamum indicum) using Illumina Platform

open access: yesThe Indian Journal of Agricultural Sciences, 2023
Sesame is an important oil seed crop worldwide and has essential health and medicinal values. In the present study, a high-throughput transcriptome sequencing of sesame was performed using Illumina paired-end sequencing technology for gene and marker ...
P SUPRIYA, A R RAO, K V BHAT
doaj   +1 more source

Insights into bacterioplankton community structure from Sundarbans mangrove ecoregion using Sanger and Illumina MiSeq sequencing approaches: A comparative analysis

open access: yesGenomics Data, 2017
Next generation sequencing using platforms such as Illumina MiSeq provides a deeper insight into the structure and function of bacterioplankton communities in coastal ecosystems compared to traditional molecular techniques such as clone library approach ...
Anwesha Ghosh, Punyasloke Bhadury
doaj   +1 more source

Complete Genome Sequence of Neisseria musculi Using Illumina and PacBio Sequencing

open access: yesMicrobiology Resource Announcements, 2021
Neisseria musculi is an oral commensal of wild-caught mice. Here, we report the complete genome sequence of N. musculi strain NW831, generated using a combination of the Illumina and PacBio platforms.
Eliza Thapa   +5 more
openaire   +2 more sources

Characterization of pseudorabies virus transcriptome by Illumina sequencing [PDF]

open access: yesBMC Microbiology, 2015
Pseudorabies virus is a widely-studied model organism of the Herpesviridae family, with a compact genome arrangement of 72 known coding sequences. In order to obtain an up-to-date genetic map of the virus, a combination of RNA-sequencing approaches were applied, as recent advancements in high-throughput sequencing methods have provided a wealth of ...
Oláh, Péter   +5 more
openaire   +3 more sources

Paired-end sequencing of Fosmid libraries by Illumina [PDF]

open access: yesGenome Research, 2012
Eliminating the bacterial cloning step has been a major factor in the vastly improved efficiency of massively parallel sequencing approaches. However, this also has made it a technical challenge to produce the modern equivalent of the Fosmid- or BAC-end sequences that were crucial for assembling and analyzing complex genomes during the Sanger-based ...
Williams, Louise J.S.   +12 more
openaire   +2 more sources

A chromosome-level genome assembly of the jade perch (Scortum barcoo)

open access: yesScientific Data, 2022
Measurement(s) whole genome sequencing Technology Type(s) Illumina Sequencing • Oxford Nanopore ...
Yishan Lu   +11 more
doaj   +1 more source

Identification of allele-specific KIV-2 repeats and impact on Lp(a) measurements for cardiovascular disease risk

open access: yesBMC Medical Genomics
The abundance of Lp(a) protein holds significant implications for the risk of cardiovascular disease (CVD), which is directly impacted by the copy number (CN) of KIV-2, a 5.5 kbp sub-region.
Sairam Behera   +25 more
doaj   +1 more source

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