Results 51 to 60 of about 1,311,956 (360)
Reliable variant calling during runtime of Illumina sequencing. [PDF]
Sci Rep, 2019 AbstractThe sequential paradigm of data acquisition and analysis in next-generation sequencing leads to high turnaround times for the generation of interpretable results. We combined a novel real-time read mapping algorithm with fast variant calling to obtain reliable variant calls still during the sequencing process.
Loka TP, Tausch SH, Renard BY.
europepmc +5 more sources
An improved genome of the model marine alga Ostreococcus tauri unfolds by assessing Illumina de novo assemblies [PDF]
, 2014 Background: Cost effective next generation sequencing technologies now enable the production of genomic datasets for many novel planktonic eukaryotes, representing an understudied reservoir of genetic diversity. O.
Blanc-Mathieu, Romain +14 more
core +6 more sources
QQ-SNV: single nucleotide variant detection at low frequency by comparing the quality quantiles [PDF]
, 2015 Background: Next generation sequencing enables studying heterogeneous populations of viral infections. When the sequencing is done at high coverage depth ("deep sequencing"), low frequency variants can be detected.
Aerssens, Jeroen +7 more
core +4 more sources
Definitions, 2020 A proprietary next-generation DNA sequencing system from Solexa that uses reversible terminator nucleotides. T he genomic DNA to be sequenced is fragmented and ligated to adapter molecules on both ends to construct an Illumina-specific adapter library ...
semanticscholar +1 more source
Comparison of SARS-CoV-2 sequencing using the ONT GridION and the Illumina MiSeq
BMC Genomics, 2022 Over 4 million SARS-CoV-2 genomes have been sequenced globally in the past 2 years. This has been crucial in elucidating transmission chains within communities, the development of new diagnostic methods, vaccines, and antivirals.
D. Tshiabuila +12 more
semanticscholar +1 more source
A Hybrid Sequencing Approach Completes the Genome Sequence of Thermoanaerobacter ethanolicus JW 200 [PDF]
, 2019 Thermoanaerobacter ethanolicus JW 200 has been identified as a potential sustainable biofuel producer due to its ability to readily ferment carbohydrates to ethanol. A hybrid sequencing approach, combining Oxford Nanopore and Illumina DNA sequence reads,
Ayine, Monica L. +9 more
core +1 more source
nCoV-2019 sequencing protocol for illumina v1 [PDF]
, 2020 This protocol is folked from "ARTIC amplicon sequencing protocol for MinION for nCoV-2019" by Josh Quick to adapt it to illumina sequencers. Because the PCR products are fragmented and ligated with adapters, this protocol is not restricted to 250 PE mode of MiSeq.
Kentaro Itokawa +4 more
openaire +2 more sources
A chromosome-level genome assembly of the jade perch (Scortum barcoo)
Scientific Data, 2022 Measurement(s) whole genome sequencing Technology Type(s) Illumina Sequencing • Oxford Nanopore ...
Yishan Lu +11 more
doaj +1 more source
Open Forum Infectious Diseases, 2022 Background Infantile central nervous system infections (CNSIs) can be life-threatening and cause severe sequelae. However, the causative microorganism remains unknown in >40% of patients with aseptic infections. This study aimed to analyze the metagenome
Kazuhiro Horiba +13 more
semanticscholar +1 more source
leeHom: adaptor trimming and merging for Illumina sequencing reads [PDF]
Nucleic Acids Research, 2014 The sequencing of libraries containing molecules shorter than the read length, such as in ancient or forensic applications, may result in the production of reads that include the adaptor, and in paired reads that overlap one another. Challenges for the processing of such reads are the accurate identification of the adaptor sequence and accurate ...
Gabriel Renaud, Udo Stenzel, Janet Kelso
openaire +3 more sources