Results 71 to 80 of about 1,311,956 (360)

Comparison of different sequencing techniques for identification of SARS-CoV-2 variants of concern with multiplex real-time PCR.

PLoS ONE, 2022
As different SARS-CoV-2 variants emerge and with the continuous evolvement of sub lineages of the delta variant, it is crucial that all countries carry out sequencing of at least >1% of their infections, in order to detect emergence of variants with ...
Diyanath Ranasinghe, Tibutius Thanesh Pramanayagam Jayadas, Deshni Jayathilaka, Chandima Jeewandara, Osanda Dissanayake, Dinuka Guruge, Dinuka Ariyaratne, Dumni Gunasinghe, Laksiri Gomes, Ayesha Wijesinghe, Ruwan Wijayamuni, Gathsaurie Neelika Malavige   +11 more
doaj   +1 more source

An improved protocol for small RNA library construction using High Definition adapters [PDF]

, 2015
Next generation sequencing of small RNA (sRNA) libraries is widely used for studying sRNAs in various biological systems. However, cDNA libraries of sRNAs are biased for molecules that are ligated to adapters more or less efficiently than other molecules.
Aravin, Baran, Bushati, Edgar, Git, Hafner, He, Jagadeeswaran, Jayaprakash, Kawano, Mallory, Mortazavi, Munafó, Pease, Prüfer, Raabe, Reddy, Song, Sorefan, Stocks, Sun, Szittya, Szittya, Tian, Vigneault, Viollet, Willenbrock, Zhang, Zhuang   +28 more
core   +1 more source

Assessment of metagenomic Nanopore and Illumina sequencing for recovering whole genome sequences of chikungunya and dengue viruses directly from clinical samples

Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin, 2018
Background The recent global emergence and re-emergence of arboviruses has caused significant human disease. Common vectors, symptoms and geographical distribution make differential diagnosis both important and challenging.
L. Kafetzopoulou, Kyriakos Efthymiadis, Kuiama Lewandowski, A. Crook, D. Carter, J. Osborne, E. Aarons, R. Hewson, J. Hiscox, M. Carroll, R. Vipond, S. Pullan   +11 more
semanticscholar   +1 more source

Utility of nanopore sequencing for detecting pathogens in bronchoalveolar lavage fluid from pediatric patients with respiratory failure

Journal of Clinical Virology Plus, 2023
RNA viruses are the most frequent pathogens responsible for respiratory infections, particularly in pediatric patients. Next-generation sequencing, represented by Illumina sequencing, is one of the most comprehensive methods for identifying pathogens ...
Makoto Yamaguchi, Kazuhiro Horiba, Kazunori Haruta, Suguru Takeuchi, Takako Suzuki, Yuka Torii, Shinji Kawabe, Sho Wada, Takanari Ikeyama, Yoshinori Ito, Tomoo Ogi, Jun-ichi Kawada   +11 more
doaj  

Comparison of DNA-, PMA-, and RNA-based 16S rRNA Illumina sequencing for detection of live bacteria in water

Scientific Reports, 2017
The limitation of 16S rRNA gene sequencing (DNA-based) for microbial community analyses in water is the inability to differentiate live (dormant cells as well as growing or non-growing metabolically active cells) and dead cells, which can lead to false ...
Ru Li, Ru Li, H. Tun, H. Tun, M. Jahan, Zhengxiao Zhang, Ayush Kumar, W. Fernando, A. Farenhorst, E. Khafipour   +9 more
semanticscholar   +1 more source

Evaluation of the reproducibility of amplicon sequencing with Illumina MiSeq platform

PLOS ONE, 2017
Illumina's MiSeq has become the dominant platform for gene amplicon sequencing in microbial ecology studies; however, various technical concerns, such as reproducibility, still exist. To assess reproducibility, 16S rRNA gene amplicons from 18 soil samples of a reciprocal transplantation experiment were sequenced on an Illumina MiSeq.
Liyou Wu, Daliang Ning, Feifei Liu, Joy D. Van Nostrand, Jizhong Zhou, Jizhong Zhou, Jizhong Zhou, Kai Xue, Ye Deng, Ye Deng, Chongqing Wen, Chongqing Wen, Bo Sun, Yujia Qin, Yuting Liang   +14 more
openaire   +7 more sources

Identification of allele-specific KIV-2 repeats and impact on Lp(a) measurements for cardiovascular disease risk

BMC Medical Genomics
The abundance of Lp(a) protein holds significant implications for the risk of cardiovascular disease (CVD), which is directly impacted by the copy number (CN) of KIV-2, a 5.5 kbp sub-region.
Sairam Behera, Jonathan R. Belyeu, Xiao Chen, Luis F. Paulin, Ngoc Quynh H. Nguyen, Emma Newman, Medhat Mahmoud, Vipin K. Menon, Qibin Qi, Parag Joshi, Santica Marcovina, Massimiliano Rossi, Eric Roller, James Han, Vitor Onuchic, Christy L. Avery, Christie M. Ballantyne, Carlos J. Rodriguez, Robert C. Kaplan, Donna M. Muzny, Ginger A. Metcalf, Richard A. Gibbs, Bing Yu, Eric Boerwinkle, Michael A. Eberle, Fritz J. Sedlazeck   +25 more
doaj   +1 more source

Resistance gene enrichment sequencing (RenSeq) enables reannotation of the NB-LRR gene family from sequenced plant genomes and rapid mapping of resistance loci in segregating populations [PDF]

, 2013
RenSeq is a NB-LRR (nucleotide binding-site leucine-rich repeat) gene-targeted, Resistance gene enrichment and sequencing method that enables discovery and annotation of pathogen resistance gene family members in plant genome sequences.
Albert, Altschul, Andolfo, Bailey, Bakker, Blankenberg, Brommonschenkel, Cock, Cronn, Dangl, Ernst, Fiume, Giardine, Goecks, Hodges, Ishibashi, Jaccoud, Jones, Jupe, Kent, Kurtz, Lanfermeijer, Li, Loveland, Lozano, Maclean, Meyers, Milligan, Ori, Park, Parla, Potato Genome Sequencing Consortium, Rauscher, Saintenac, Schornack, Schulze-Lefert, Tai, The Arabidopsis Genome Initiative, Tomato Genome Consortium, Weigel, Yandell, Zerbino, Zhang, Śliwka   +43 more
core   +3 more sources

Comparative performance of the BGI and Illumina sequencing technology for single-cell RNA-sequencing

bioRxiv, 2019
The libraries generated by high-throughput single cell RNA-sequencing platforms such as the Chromium from 10x Genomics require considerable amounts of sequencing, typically due to the large number of cells.
A. Senabouth, Stacey B. Andersen, Qianyu Shi, Lei Shi, Feng Jiang, Wenwei Zhang, Kristof Wing, Maciej S Daniszewski, S. Lukowski, Sandy S. C. Hung, Q. Nguyen, L. Fink, A. Beckhouse, A. Pébay, A. Hewitt, J. Powell   +15 more
semanticscholar   +1 more source

nifH amplification for Illumina sequencing v1

, 2020
For metabarcoding purpose, the first step involves the amplification by PCR of a given gene region (for example V4 or V9 region of 18S rRNA gene) or gene itself if its size does not exceed 600bp (the longest fragment size that can be sequenced by ...
E. Bigeard, Adriana Lopes dos Santos, C. Ribeiro   +2 more
semanticscholar   +1 more source