Results 41 to 50 of about 309,496 (294)

Rapid metaphase and interphase detection of radiation-induced chromosome aberrations in human lymphocytes by chromosomal suppression in situ hybridization [PDF]

open access: yes, 1990
Chromosomal in situ suppression (CISS)-hybridization of biotinylated phage DNA-library inserts from sorted human chromosomes was used to decorate chromosomes 1 and 7 specifically from pter to qter and to detect structural aberrations of these chromosomes
Cremer, Christoph   +4 more
core   +1 more source

Treating Macrophages with Anti-inflammatory Nanoparticles as a Strategy to Improve Muscle Repair [PDF]

open access: yes, 2019
The macrophage is an immune cell that is involved in host defense. More recent research, however, has revealed that they also play a central role in mediating the skeletal muscle regenerative process.
Yan, Derek
core   +1 more source

Enteropathogenic E. coli shows delayed attachment and host response in human jejunum organoid‐derived monolayers compared to HeLa cells

open access: yesFEBS Letters, EarlyView.
Enteropathogenic E. coli (EPEC) infects the human intestinal epithelium, resulting in severe illness and diarrhoea. In this study, we compared the infection of cancer‐derived cell lines with human organoid‐derived models of the small intestine. We observed a delayed in attachment, inflammation and cell death on primary cells, indicating that host ...
Mastura Neyazi   +5 more
wiley   +1 more source

Brewing yeast viability measured using a novel fluorescent dye and image cytometer

open access: yesBiotechnology & Biotechnological Equipment, 2019
This study presents an automated, image-based cytometry method for determination of total counts and viability of yeast cells by using a newly synthesized DNA fluorescent dye PO-TEDM-1 and a new Easycounter YC instrument.
Milka Atanasova   +5 more
doaj   +1 more source

An Experimental Insight into Extracellular Phosphatases – Differential Induction of Cell-Specific Activity in Green Algae Cultured under Various Phosphorus Conditions

open access: yesFrontiers in Microbiology, 2018
Extracellular phosphatase activity (PA) has been used as an overall indicator of P depletion in lake phytoplankton. However, detailed insights into the mechanisms of PA regulation are still limited, especially in the case of acid phosphatases.
Jaroslav Vrba   +6 more
doaj   +1 more source

J Fluorescence [PDF]

open access: yes, 2005
The scope of this paper is to illustrate the need for an improved quality assurance in fluorometry. For this purpose, instrumental sources of error and their influences on the reliability and comparability of fluorescence data are highlighted for ...
Ebert, P.   +7 more
core   +2 more sources

Crosstalk between the ribosome quality control‐associated E3 ubiquitin ligases LTN1 and RNF10

open access: yesFEBS Letters, EarlyView.
Loss of the E3 ligase LTN1, the ubiquitin‐like modifier UFM1, or the deubiquitinating enzyme UFSP2 disrupts endoplasmic reticulum–ribosome quality control (ER‐RQC), a pathway that removes stalled ribosomes and faulty proteins. This disruption may trigger a compensatory response to ER‐RQC defects, including increased expression of the E3 ligase RNF10 ...
Yuxi Huang   +8 more
wiley   +1 more source

Image3C, a multimodal image-based and label-independent integrative method for single-cell analysis

open access: yeseLife, 2021
Image-based cell classification has become a common tool to identify phenotypic changes in cell populations. However, this methodology is limited to organisms possessing well-characterized species-specific reagents (e.g., antibodies) that allow cell ...
Alice Accorsi   +5 more
doaj   +1 more source

CD24 signalling through macrophage Siglec-10 is a target for cancer immunotherapy. [PDF]

open access: yes, 2019
Ovarian cancer and triple-negative breast cancer are among the most lethal diseases affecting women, with few targeted therapies and high rates of metastasis. Cancer cells are capable of evading clearance by macrophages through the overexpression of anti-
Barkal, Amira A   +10 more
core  

Detection and quantification of viable airborne bacteria and fungi using solid-phase cytometry [PDF]

open access: yes, 2009
This protocol describes the use of solid-phase cytometry for the enumeration of airborne bacteria and fungi. In contrast with conventional methods, accurate results can be obtained in real time, especially for air samples with low numbers of ...
Coenye, Tom, Nelis, Hans, Vanhee, Lies
core   +1 more source

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