Results 41 to 50 of about 195,947 (313)

Single-cell western blotting. [PDF]

, 2014
To measure cell-to-cell variation in protein-mediated functions, we developed an approach to conduct ∼10(3) concurrent single-cell western blots (scWesterns) in ∼4 h.
Herr, Amy E, Hughes, Alex J, Kang, Chi-Chih, Schaffer, David V, Spelke, Dawn P, Xu, Zhuchen   +5 more
core   +2 more sources

A platinum polymer probe for multiplexed single cell suspension mass cytometry and imaging mass cytometry assays

Chemical Engineering Journal
Edmond C. N. Wong, Tianjia Yang, Yefeng Zhang, Yang Liu, Daniel Majonis, Mahtab Abtahi, Chen Xu, Xiaochong Li, Peter John Hawrysh, Taunia Closson, Shelly Paluan, Mitchell A. Winnik   +11 more
openalex   +2 more sources

Tissue Staining for Imaging Mass Cytometry v1 [PDF]

, 2021
This protocol describes the preparation and staining of human FFPE material for multiplex visualization using Imaging Mass Cytometry (IMC). This protocol uses basic standard immunohistochemical staining techniques. The tissue is incubated with antibodies that have specific affinity for different cells and tissues in the context of a formalin fixed ...
John Herndon, Madelyn Carmody, Stephen Oh, not provided William Gillanders Washington University St Louis   +3 more
openaire   +1 more source

MATISSE: a method for improved single cell segmentation in imaging mass cytometry

BMC Biology, 2021
Background Visualizing and quantifying cellular heterogeneity is of central importance to study tissue complexity, development, and physiology and has a vital role in understanding pathologies.
Matthijs J. D. Baars, Neeraj Sinha, Mojtaba Amini, Annelies Pieterman-Bos, Stephanie van Dam, Maroussia M. P. Ganpat, Miangela M. Laclé, Bas Oldenburg, Yvonne Vercoulen   +8 more
doaj   +1 more source

High Dimensional Imaging Mass Cytometry Panel to Visualize the Tumor Immune Microenvironment Contexture

Frontiers in Immunology, 2021
The integrative analysis of tumor immune microenvironment (TiME) components, their interactions and their microanatomical distribution is mandatory to better understand tumor progression.
Roxane Elaldi, Roxane Elaldi, Patrice Hemon, Luciana Petti, Estelle Cosson, Belinda Desrues, Anne Sudaka, Gilles Poissonnet, Ellen Van Obberghen-Schilling, Jacques-Olivier Pers, Veronique M. Braud, Fabienne Anjuère, Aïda Meghraoui-Kheddar   +12 more
doaj   +1 more source

Site Selective Antibody-Oligonucleotide Conjugation via Microbial Transglutaminase. [PDF]

, 2019
Nucleic Acid Therapeutics (NATs), including siRNAs and AntiSense Oligonucleotides (ASOs), have great potential to drug the undruggable genome. Targeting siRNAs and ASOs to specific cell types of interest has driven dramatic improvement in efficacy and ...
Cui, Xianshu, Dowdy, Steven F, Huggins, Ian J, Jadhav, Satish, Medina, Carlos A, Springer, Aaron D, van den Berg, Arjen   +6 more
core   +1 more source

Graph of graphs analysis for multiplexed data with application to imaging mass cytometry.

PLoS Computational Biology, 2021
Imaging Mass Cytometry (IMC) combines laser ablation and mass spectrometry to quantitate metal-conjugated primary antibodies incubated in intact tumor tissue slides.
Ya-Wei Eileen Lin, Tal Shnitzer, Ronen Talmon, Franz Villarroel-Espindola, Shruti Desai, Kurt Schalper, Yuval Kluger   +6 more
doaj   +1 more source

Highly multiplexed 2-dimensional imaging mass cytometry analysis of HBV-infected liver

JCI Insight, 2021
Studies of human hepatitis B virus (HBV) immune pathogenesis are hampered by limited access to liver tissues and technologies for detailed analyses. Here, utilizing imaging mass cytometry (IMC) to simultaneously detect 30 immune, viral, and structural ...
Daniel Traum, Yue J. Wang, Kathleen B. Schwarz, Jonathan Schug, David K.H. Wong, Harry L.A. Janssen, Norah A. Terrault, Mandana Khalili, Abdus S. Wahed, Karen F. Murray, Phillip Rosenthal, Simon C. Ling, Norberto Rodriguez-Baez, Richard K. Sterling, Daryl T.Y. Lau, Timothy M. Block, Michael D. Feldman, Elizabeth E. Furth, William M. Lee, David E. Kleiner, Anna S. Lok, Klaus H. Kaestner, Kyong-Mi Chang   +22 more
doaj   +1 more source

A 34-Marker Panel for Imaging Mass Cytometric Analysis of Human Snap-Frozen Tissue

Frontiers in Immunology, 2020
Imaging mass cytometry (IMC) is able to quantify the expression of dozens of markers at sub-cellular resolution on a single tissue section by combining a novel laser ablation system with mass cytometry.
Nannan Guo, Vincent van Unen, Vincent van Unen, Marieke E. Ijsselsteijn, Laura F. Ouboter, Andrea E. van der Meulen, Susana M. Chuva de Sousa Lopes, Noel F. C. C. de Miranda, Frits Koning, Na Li, Na Li   +10 more
doaj   +1 more source

ATR inhibition facilitates targeting of leukemia dependence on convergent nucleotide biosynthetic pathways. [PDF]

, 2017
Leukemia cells rely on two nucleotide biosynthetic pathways, de novo and salvage, to produce dNTPs for DNA replication. Here, using metabolomic, proteomic, and phosphoproteomic approaches, we show that inhibition of the replication stress sensing kinase ...
Abt, Evan R, Braas, Daniel, Capri, Joseph R, Cheng, Chloe M, Czernin, Johannes, Donahue, Timothy R, Faull, Kym F, Herschman, Harvey R, Kim, Woosuk, Kornblum, Harley I, Le, Thuc M, Merkurjev, Daria, Nikanjam, Mina, Poddar, Soumya, Radu, Caius G, Ribas, Antoni, Rix, Peter, Uong, Nhu T, Wei, Liu, Whitelegge, Julian, Zaretsky, Jesse   +20 more
core   +2 more sources