Results 51 to 60 of about 149,839 (140)

ImmuVis: Hyperconvolutional Foundation Model for Imaging Mass Cytometry

CoRR
We present ImmuVis, a family of efficient foundation models for imaging mass cytometry (IMC), a high-throughput multiplex imaging technology that handles molecular marker measurements as image channels and enables large-scale spatial tissue profiling. Unlike natural images, multiplex imaging lacks a fixed channel space, as real-world marker sets vary ...
Marcin Mozejko, Dawid Uchal, Krzysztof Gogolewski, Piotr Kupidura, Szymon Lukasik, Jakub Giezgala, Tomasz Nocon, Kacper Pietrzyk, Robert Pieniuta, Mateusz Sulimowicz, Michal Orzylowski, Tomasz Silkowski, Karol Zagródka, Eike Staub, Ewa Szczurek   +14 more
openaire   +3 more sources

Pushing the Limits of Cell Segmentation Models for Imaging Mass Cytometry

2024 IEEE International Symposium on Biomedical Imaging (ISBI)
Imaging mass cytometry (IMC) is a relatively new technique for imaging biological tissue at subcellular resolution. In recent years, learning-based segmentation methods have enabled precise quantification of cell type and morphology, but typically rely on large datasets with fully annotated ground truth (GT) labels.
Kimberley M. Bird, Xujiong Ye, Alan M. Race, James M. Brown 0001   +3 more
openaire   +4 more sources

Imaging Mass Cytometry Compensation Slide Acquisition v1 [PDF]

, 2020
This SOP describes the slide acquisition for spill-over correction of Imaging Mass Cytometry experiments. The protocol makes use of the compensation slide, which was prepared previously following the SOP: “HuBMAP_Protocol_3_Compensation_Slide_Preparation.pdf” to measure each conjugated antibody individually and to record all observed spill-over.
Michelle Daniel, Marda Jorgensen
openaire   +1 more source

Cutting FFPE Sections for Imaging Mass Cytometry v1 [PDF]

, 2020
FFPE block are cut into thin sections.
Marda Jorgensen, Michelle Daniel
openaire   +1 more source

Multimodal Mass Spectrometry Imaging of an Osteosarcoma Multicellular Tumour Spheroid Model to Investigate Drug-Induced Response

Metabolites
A multimodal mass spectrometry imaging (MSI) approach was used to investigate the chemotherapy drug-induced response of a Multicellular Tumour Spheroid (MCTS) 3D cell culture model of osteosarcoma (OS). The work addresses the critical demand for enhanced
Sophie M. Pearce, Neil A. Cross, David P. Smith, Malcolm R. Clench, Lucy E. Flint, Gregory Hamm, Richard Goodwin, James I. Langridge, Emmanuelle Claude, Laura M. Cole   +9 more
doaj   +1 more source

Oligonucleotide-polymer conjugation for imaging mass cytometry v2

, 2023
This protocol describes the procedures to label 5'-thiol modified oligonucleotides with maleimide carrying polymers which are also chelated with metal-ions. This is the basis to perform RNAscope with Imaging Mass Cytometry since therein we use metal readouts instead of fluorescence readouts. This protocol has been used in the following publications:
openaire   +1 more source

The phenogenomic landscapes of pleural mesothelioma tumor microenvironment predict clinical outcomes

Journal of Translational Medicine
Background Malignant pleural mesothelioma (MPM) is a rare and aggressive malignancy with limited therapeutic options. To improve patients management and treatment, more precise stratification strategies are needed.
Federica Torricelli, Filomena Spada, Cynthia Bishop, Katrina Todd, Daisuke Nonaka, Nedyalko Petrov, Manuela Terranova Barberio, Alan G. Ramsay, Richard Ellis, Alessia Ciarrocchi, Benedetta Apollonio, Andrea Billè   +11 more
doaj   +1 more source

Staining protocol for Imaging Mass Cytometry v1

In Imaging Mass Cytometry (IMC), a high-resolution laser is combined with a mass cytometer that permits mass spectrometry-based, spatially reserved high-dimensional analysis of intact formalin-fixed paraffin-embedded (FFPE) tissues. The protocol summarizes the staining procedure for IMC using a cocktail of heavy metal conjugated primary antibodies ...
Vijayakumar R Kakade, Tifanny Budiman, Lloyd Cantley   +2 more
openaire   +1 more source

Mass cytometry: exploring the immune landscape of systemic autoimmune and inflammatory diseases in the past fourteen years

Frontiers in Immunology
Auto-immune and inflammatory diseases are heterogenous in their clinical manifestations and prognosis, even among individuals presenting with the same pathology. Understanding the immunological alterations involved in their pathogenesis provides valuable
Aïcha Kante, Aïcha Kante, Mathieu F. Chevalier, Damien Sène, Damien Sène, Jeanne Chauffier, Jeanne Chauffier, Stéphane Mouly, Stéphane Mouly, Benjamin Glenn Chousterman, Benjamin Glenn Chousterman, Fériel Azibani, Benjamin Terrier, Benjamin Terrier, Théo Pezel, Théo Pezel, Cloé Comarmond, Cloé Comarmond   +17 more
doaj   +1 more source

Development of an analytical system for renal spatial immune microenvironment using imaging mass cytometry

陆军军医大学学报
Objective The progression of chronic kidney disease (CKD) is closely associated with the disruption of renal spatial immune microenvironment. This study aimed to develop an integrated experimental and analytical system for dissecting the immune ...
LIU Jiawen, XIANG Qun
doaj   +1 more source