Results 51 to 60 of about 141,877 (234)

Erythropoietin modulates hepatic inflammation, glucose homeostasis, and soluble epoxide hydrolase and epoxides in high‐fat diet‐induced obese mice

FEBS Open Bio, EarlyView.
Erythropoietin administration suppresses hepatic soluble epoxide hydrolase (sEH) expression, leading to increased CYP‐derived epoxides. This is associated with a shift in hepatic macrophage polarization characterized by reduced M1 markers and increased M2 markers, along with reduced hepatic inflammation, suppressed hepatic lipogenesis, and attenuated ...
Takeshi Goda, Satoru Sugimoto, Chiharu Cho, Madoka Konishi, Nozomi Inoue, Satoshi Miyagaki, Yasuhiro Kawabe, Takuro Okamura, Masahide Hamaguchi, Hisakazu Nakajima, Michiaki Fukui, Masakazu Shinohara, Tomoko Iehara   +12 more
wiley   +1 more source

Exon 7 splicing of ERα predicts poor prognosis and increases phenotypic heterogeneity in luminal a subtype breast cancer

FEBS Open Bio, EarlyView.
ERα splice variant ERα∆7 lacks the C‐terminus, and its expression may change phenotypes of breast cancers. Our results showed that ERα∆7 is found in the luminal A subtype, and elevated ERα∆7 levels are linked to improved cell survival with lower proliferation and migration.
Long Wai Tsui, Taobo Hu, Thomson Christian Husein, Wai Hei Shek, Bingrong Chen, Depeng Wang, Shu Wang, Weichuan Yu, Xiaodan Fan, Mengping Long, Toyotaka Ishibashi   +10 more
wiley   +1 more source

ImaCytE: Visual Exploration of Cellular Micro-Environments for Imaging Mass Cytometry Data

, 2021
Tissue functionality is determined by the characteristics of tissue-resident cells and their interactions within their microenvironment. Imaging Mass Cytometry offers the opportunity to distinguish cell types with high precision and link them to their ...
Koning, F., van Unen, Vincent (author), Lelieveldt, B.P.F. (author), Höllt, T. (author), Koning, Frits (author), Somarakis, A., Somarakis, Antonios (author), Unen, V. van, Lelieveldt, B.P.F., van Unen, Vincent, Somarakis, Antonios, Koning, Frits, Lelieveldt, B., Hollt, T.   +13 more
core   +1 more source

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

Imaging Mass Cytometry Compensation Slide Acquisition v1 [PDF]

, 2020
This SOP describes the slide acquisition for spill-over correction of Imaging Mass Cytometry experiments. The protocol makes use of the compensation slide, which was prepared previously following the SOP: “HuBMAP_Protocol_3_Compensation_Slide_Preparation.pdf” to measure each conjugated antibody individually and to record all observed spill-over.
Michelle Daniel, Marda Jorgensen
openaire   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

TICAL - a web-tool for multivariate image clustering and data topology preserving visualization [PDF]

, 2011
In life science research bioimaging is often used to study two kinds of features in a sample simultaneously: morphology and co-location of molecular components.
Abouna, Sylvie, Khan, Michael, Nattkemper, Tim W., Langenkämper, Daniel, Niehaus, Karsten, Kölling, Jan   +5 more
core   +2 more sources

Imaging Mass Cytometry Antibody Staining v1

, 2020
The following SOP describes procedures required for the antibody staining of formalin fixed, paraffin-embedded (FFPE) samples. In this SOP, the term “samples” refers to FFPE tissue samples.
Michelle Daniel, Marda Jorgensen
openaire   +1 more source

Imaging Mass Cytometry Compensation Slide Preparation v1 [PDF]

, 2020
This SOP describes the preparation of compensation slides with single antibodyconjugate spots that can be used to estimate mass channel spillover for IMC. Spillover varies from metal lot to lot due to varying impurities. Once a spillover matrix for a specific set of antibody-conjugates has been generated, the experiment of interest, in which the same ...
Michelle Daniel, Marda Jorgensen
openaire   +1 more source

Derivation and characterization of retinal pigment epithelium from urine‐derived iPSCs

FEBS Open Bio, EarlyView.
Age‐related macular degeneration causes vision loss via RPE dysfunction and loss. Traditional iPSC therapies rely on invasive biopsies, limiting scalability. Here, we utilize urine‐derived stem cells as an accessible source to generate u‐iPSCs, successfully differentiated into pigmented RPE. This “Urine‐to‐Retina” platform provides a promising path for
Daniella Beiner, Hainan Zhu, Carol Christine Bosholm, Heuy‐Ching Wang, Tracy Criswell, Anthony Atala, Jian‐Xing Ma, Yuanyuan Zhang   +7 more
wiley   +1 more source