Results 1 to 10 of about 5,255,854 (171)

Immuno Tomography (IT) and Imaging Mass Cytometry (IMC) for constructing spatially resolved, multiplexed 3D IMC data sets. [PDF]

Ocul Surf, 2022
We have previously used Immuno Tomography (IT) to identify label-retaining stem cell populations in the cornea and meibomian gland. While this method provides the unique ability to quantify stem cell populations comprised of 1-4 cells, the number of antigens that can be sequentially used to characterize these unique cells is limited by antigen ...
Gheiratmand L, Brown DJ, Sandkuijl D, Loboda A, Jester JV.   +4 more
europepmc   +9 more sources

IMC-Denoise: a content aware denoising pipeline to enhance Imaging Mass Cytometry [PDF]

Nature Communications, 2023
Multiplexed imaging technologies can reveal the complex cellular and molecular profiles of tissue. Here, the authors develop and implement a denoising pipeline to significantly enhance imaging mass cytometry quality and improve single-cell analyses.
Peng Lu, Karolyn A. Oetjen, Diane E. Bender, Marianna B. Ruzinova, Daniel A. C. Fisher, Kevin G. Shim, Russell K. Pachynski, W. Nathaniel Brennen, Stephen T. Oh, Daniel C. Link, Daniel L. J. Thorek   +10 more
doaj   +7 more sources

Imaging mass cytometry for high-dimensional tissue profiling in the eye

BMC Ophthalmology, 2021
Background Imaging mass cytometry (IMC) combines the principles of flow cytometry and mass spectrometry (MS) with laser scanning spatial resolution and offers unique advantages for the analysis of tissue samples in unprecedented detail.
Anja Schlecht, Stefaniya Boneva, Henrike Salie, Saskia Killmer, Julian Wolf, Rozina Ida Hajdu, Claudia Auw-Haedrich, Hansjürgen Agostini, Thomas Reinhard, Günther Schlunck, Bertram Bengsch, Clemens AK Lange   +11 more
doaj   +2 more sources

Dual-modality imaging of immunofluorescence and imaging mass cytometry for whole-slide imaging and accurate segmentation

Cell Reports: Methods, 2023
Summary: Imaging mass cytometry (IMC) is a powerful technique capable of detecting over 30 markers on a single slide. It has been increasingly used for single-cell-based spatial phenotyping in a wide range of samples.
Eun Na Kim, Phyllis Zixuan Chen, Dario Bressan, Monika Tripathi, Ahmad Miremadi, Massimiliano di Pietro, Lisa M. Coussens, Gregory J. Hannon, Rebecca C. Fitzgerald, Lizhe Zhuang, Young Hwan Chang   +10 more
doaj   +3 more sources

A pilot imaging mass cytometry study of cutaneous nerve-immune cell interactions in post-herpetic neuralgia [PDF]

Frontiers in Immunology
Post-herpetic neuralgia (PHN) is a chronic pain condition that persists after shingles and involves dermatome-specific allodynia, disability, and psychological comorbidities. Cutaneous neuroimmune dysregulation is increasingly linked to chronic pain, but
Siddhesh N. Telang, Siddhesh N. Telang, Jackson F. Karrasch, Jackson F. Karrasch, Jackson F. Karrasch, Philip M. Finch, Philip M. Finch, Stefanie S. Kogias, Stefanie S. Kogias, Thomas R. O’Neil, Thomas R. O’Neil, Anthony L. Cunningham, Anthony L. Cunningham, Andrew N. Harman, Andrew N. Harman, Peter D. Drummond, Paul J. Austin, Paul J. Austin, Paul J. Austin   +18 more
doaj   +2 more sources

OpenIMC: an open-source platform for analyzing single-cell and spatial proteomics by imaging mass cytometry [PDF]

BMC Bioinformatics
Background Imaging Mass Cytometry (IMC) enables highly multiplexed, spatially resolved single-cell proteomics, providing simultaneous measurement of dozens of protein markers while preserving tissue architecture.
Dean Tessone, Mohamed Kamal, Valerie Hennes, Ahmed H. Saadawy, E. Shelley Hwang, Jorge Nieva, James Hicks, Peter Kuhn   +7 more
doaj   +2 more sources

SCLN: Stacked Channel Learning Network for Imaging Mass Cytometry (IMC) cell segmentation

Med-X
Abstract Imaging mass cytometry (IMC) is a high-dimensional imaging technology that allows the capture and quantification of up to fifty biomarkers at sub-cellular resolution. Each IMC dimension (‘channel’) corresponds to antibodies coupled to metal-tagged antigens and captures the imaging representation of biomarkers for each cell ...
Jiadi Dong, Tian Xia, Pradeeba Sridar, Mark Read, Thomas Ashhurst, Lei Bi, Jinman Kim   +6 more
openaire   +3 more sources

Compressed sensing expands the multiplexity of imaging mass cytometry [PDF]

Nature Communications
The multiplexity of current antibody-based imaging is limited by the number of reporters that can be detected simultaneously. Compressed sensing can be used to reconstruct high-dimensional information from low-dimensional measurements.
Tsuyoshi Hosogane, Leonor Schubert Santana, Nils Eling, Holger Moch, Bernd Bodenmiller   +4 more
doaj   +2 more sources

Pan-cancer convergence of tumour–immune microenvironment motifs revealed by CyTOF and imaging mass cytometry

Frontiers in Immunology
Mass cytometry (CyTOF) and Imaging Mass Cytometry (IMC) provide single-cell resolution for over 50 protein markers, enabling unprecedented exploration of tumour and immune heterogeneity.
Alexandre Vallée, Alexandre Vallée, Alexandre Drezet, Maxence Arutkin   +3 more
doaj   +3 more sources

Dice-XMBD: Deep Learning-Based Cell Segmentation for Imaging Mass Cytometry

Frontiers in Genetics, 2021
Highly multiplexed imaging technology is a powerful tool to facilitate understanding the composition and interactions of cells in tumor microenvironments at subcellular resolution, which is crucial for both basic research and clinical applications ...
Xu Xiao, Xu Xiao, Ying Qiao, Yudi Jiao, Na Fu, Wenxian Yang, Liansheng Wang, Rongshan Yu, Rongshan Yu, Rongshan Yu, Jiahuai Han, Jiahuai Han   +11 more
doaj   +1 more source