Results 41 to 50 of about 4,996,457 (177)

Mass Spectrometry Imaging and Integration with Other Imaging Modalities for Greater Molecular Understanding of Biological Tissues

, 2018
Over the last two decades, mass spectrometry imaging (MSI) has been increasingly employed to investigate the spatial distribution of a wide variety of molecules in complex biological samples.
Siegel, Tiffany Porta, Siegel, T. P., Hamm, G., Bunch, J., Bunch, Josephine, Schwamborn, Kristina, Cappell, J., Hamm, Gregory, Fletcher, John S.,, Fletcher, John S., Cappell, Jo, Schwamborn, K.   +11 more
core   +1 more source

High-mass-resolution MALDI mass spectrometry imaging of metabolites from formalin-fixed paraffin-embedded tissue. [PDF]

, 2016
Formalin-fixed and paraffin-embedded (FFPE) tissue specimens are the gold standard for histological examination, and they provide valuable molecular information in tissue-based research.
McDonnell, L.A., Kuppen, P.J.K., Ly, A., Velde, C.J.H. van de, Kuppen, P.J., Kuppen, Peter J K, Buck, Achim, van de Velde, Cornelis J. H., Walch, A.K., Langer, R., Feuchtinger, A., Walch, A., Sun, Na, Janssen, Klaus-Peter, Balluff, Benjamin, Gorzolka, Karin, Weirich, Gregor, Langer, Rupert, Erlmeier, Franziska, Kuppen, Peter J. K., Aubele, M., Weirich, G., McDonnell, Liam, Walch, Axel, Zitzelsberger, Horst, Erlmeier, F., Ly, Alice, Sun, N., van de Velde, Cornelis J H, Aichler, Michaela, Buck, A., van de Velde, C.J., Feuchtinger, Annette, Zitzelsberger, H., Balluff, B., Aichler, M., McDonnell, L., Janssen, K.P., Aubele, Michaela, Gorzolka, K.   +39 more
core   +1 more source

SCLN: Stacked Channel Learning Network for Imaging Mass Cytometry (IMC) cell segmentation

Med-X
Abstract Imaging mass cytometry (IMC) is a high-dimensional imaging technology that allows the capture and quantification of up to fifty biomarkers at sub-cellular resolution. Each IMC dimension (‘channel’) corresponds to antibodies coupled to metal-tagged antigens and captures the imaging representation of biomarkers for each cell ...
Jiadi Dong, Tian Xia, Pradeeba Sridar, Mark Read, Thomas Ashhurst, Lei Bi, Jinman Kim   +6 more
openaire   +1 more source

Signal Amplification for Imaging Mass Cytometry

, 2019
An enzyme-catalyzed reporter deposition stain has been developed for Imaging Mass Cytometry (IMC). The reagent consists of an alkaline phosphatase substrate tethered to a tellurophene which serves as reporter group for mass cytometry.
Jay Bassan (7339763), Mark Nitz (208005), Rodolfo F. Gómez-Biagi (7828271), Rahul Rana (7828268)   +3 more
core   +1 more source

Metal Isotope-Tagged Nanomaterials for Mass Cytometry and Imaging: Advances and Biomedical Applications

Nano Biomedicine and Engineering
Mass cytometry (cytometry by time-of-flight (CyTOF)) and imaging mass cytometry (IMC) are transformative technologies that combine flow cytometry principles with time-of-flight mass spectrometry (TOF-MS).
Dongling Jia, Minhui Cui, Weiwei Wang, Jielin Sun, Xiaoxiang Chen   +4 more
doaj   +1 more source

Rare osteosarcoma cell subpopulation protein array and profiling using imaging mass cytometry and bioinformatics analysis

BMC Cancer, 2020
Background Single rare cell characterization represents a new scientific front in personalized therapy. Imaging mass cytometry (IMC) may be able to address all these questions by combining the power of MS-CyTOF and microscopy.
Izhar S. Batth, Qing Meng, Qi Wang, Keila E. Torres, Jared Burks, Jing Wang, Richard Gorlick, Shulin Li   +7 more
doaj   +1 more source

Single-cell analysis of the human pancreas in type 2 diabetes using multi-spectral imaging mass cytometry

Cell Reports, 2021
Summary: Type 2 diabetes mellitus (T2D) is a chronic age-related disorder characterized by hyperglycemia due to the failure of pancreatic beta cells to compensate for increased insulin demand.
Minghui Wu, Michelle Y.Y. Lee, Varun Bahl, Daniel Traum, Jonathan Schug, Irina Kusmartseva, Mark A. Atkinson, Guanjie Fan, Klaus H. Kaestner   +8 more
doaj   +1 more source

CD14 Plays a Critical Role in Pain and Inflammation Across Multiple Models of Post‐traumatic Osteoarthritis

Arthritis &Rheumatology, EarlyView.
Objective We employed global genetic deletion of CD14 and intra‐articular CD14 blockade across multiple murine osteoarthritis (OA) models that vary in severity of pathology and rate of progression to test the hypothesis that CD14 inhibition attenuates synovial inflammation and associated pain during disease progression.
Kevin G. Burt, Sanique M. South, Natalie S. Adamczyk, Vu Nguyen, Shingo Ishihara, Cheng Zhou, Kate L. Sharp, Sung Y. Kim, Anna E. Rapp, Baofeng Hu, Lance A. Murphy, Li‐Yin Hung, De'Broski Herbert, Joshua F. Baker, Robert L. Mauck, Timothy M. Griffin, Anne‐Marie Malfait, Rachel E. Miller, Carla R. Scanzello   +18 more
wiley   +1 more source

Multiplexed imaging of immune cells in staged multiple sclerosis lesions by mass cytometry

eLife, 2019
Multiple sclerosis (MS) is characterized by demyelinated and inflammatory lesions in the brain and spinal cord that are highly variable in terms of cellular content.
Valeria Ramaglia, Salma Sheikh-Mohamed, Karen Legg, Calvin Park, Olga L Rojas, Stephanie Zandee, Fred Fu, Olga Ornatsky, Eric C Swanson, David Pitt, Alexandre Prat, Trevor D McKee, Jennifer L Gommerman   +12 more
doaj   +1 more source

Organoid Coculture Models for Cancer Research and Immunotherapy

Med Research, EarlyView.
This review summarizes organoid coculture platforms, including submerged Matrigel culture, air–liquid interface, microfluidic/organoid‐on‐a‐chip, and 3D bioprinting, for reconstructing the tumor microenvironment to study tumor–stroma/immune crosstalk, evaluate immunotherapies, and enable drug screening.
Zhuo Yang, Huihui Yang, Guangxin Li, Guobang Liu, Zhulan Chen, Wenzhi Tian, Meiyang Huang, Xiaoling Liu, Yongsheng Zhao, Dong Chen   +9 more
wiley   +1 more source