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Compensation of Signal Spillover in Suspension and Imaging Mass Cytometry [PDF]

open access: yesCell Systems, 2018
Raw mass cytometry and imaging mass cytometry data used to develop the compensation tool available within the R package ...
Stéphane Chevrier   +2 more
exaly   +7 more sources

Ruthenium counterstaining for imaging mass cytometry

open access: yesJournal of Pathology, 2018
Imaging mass cytometry is a novel imaging modality that enables simultaneous antibody-based detection of >40 epitopes and molecules in tissue sections at subcellular resolution by the use of isotopically pure metal tags.
Raúl Catena   +2 more
exaly   +5 more sources

Different approaches to Imaging Mass Cytometry data analysis [PDF]

open access: yesBioinform Adv, 2023
Imaging Mass Cytometry (IMC) is a novel, high multiplexing imaging platform capable of simultaneously detecting and visualizing up to 40 different protein targets.
Milosevic, Vladan
core   +6 more sources

Imaging Mass Cytometry for high-dimensional tissue profiling in the eye [PDF]

open access: yesBMC Ophthalmol, 2021
Imaging mass cytometry data (folders containing single tiffs + cell masks) generated for the analysis of healthy conjunctiva and conjunctival ...
Reinhard Thomas   +11 more
core   +8 more sources

Compressed sensing expands the multiplexity of imaging mass cytometry. [PDF]

open access: yesNat Commun, 2023
Abstract The multiplexity of current antibody-based imaging is limited by the number of reporters that can be detected simultaneously. Compressed sensing can be used to recover high-dimensional information from low-dimensional measurements when the data has a structure that allows sparse representation.
Hosogane T   +4 more
europepmc   +3 more sources

DNA-Conjugated Gold Nanoparticles as High-Mass Probes in Imaging Mass Cytometry

open access: yesACS Applied Bio Materials, 2019
We employ imaging mass cytometry (IMC) to investigate in vitro uptake and cellular distribution of DNA-functionalized gold nanoparticles (AuNPs). IMC enables the multiparametric imaging of cell components and allows for the detection of AuNPs in cells ...
Jelena Brkic   +2 more
exaly   +4 more sources

Imaging Mass Cytometry [PDF]

open access: yesCytometry Part A: the Journal of the International Society for Analytical Cytology, 2017
AbstractImaging Mass Cytometry (IMC) is an expansion of mass cytometry, but rather than analyzing single cells in suspension, it uses laser ablation to generate plumes of particles that are carried to the mass cytometer by a stream of inert gas. Images reconstructed from tissue sections scanned by IMC have a resolution comparable to light microscopy ...
Olga I Ornatsky   +2 more
exaly   +3 more sources

Signal Amplification for Imaging Mass Cytometry

open access: yesBioconjugate Chemistry, 2019
An enzyme-catalyzed reporter deposition stain has been developed for Imaging Mass Cytometry (IMC). The reagent consists of an alkaline phosphatase substrate tethered to a tellurophene which serves as reporter group for mass cytometry.
Jay Bassan (7339763)   +3 more
core   +3 more sources

Imaging mass cytometry reveals tissue-specific cellular immune phenotypes in the mouse knee following ACL injury [PDF]

open access: yesOsteoarthr Cartil Open, 2023
Objective: To develop an imaging mass cytometry method for identifying complex cell phenotypes, inter-cellular interactions, and population changes in the synovium and infrapatellar fat pad (IFP) of the mouse knee following a non-invasive compression ...
M. Caleb Marlin   +8 more
core   +2 more sources

An imaging mass cytometry immunophenotyping panel for non-human primate tissues [PDF]

open access: yesFront Immunol, 2022
It has recently become clear that spatial organization contributes to cellular function and that expanding our knowledge on cellular organization is essential to further our understanding of processes in health and disease. Imaging mass cytometry enables
Ottenhoff, T.H.M.   +9 more
core   +2 more sources

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