Results 41 to 50 of about 149,839 (140)

Single-Cell Analysis of Refractory Celiac Disease Demonstrates Inter- and Intra-Patient Aberrant Cell HeterogeneitySummary

Cellular and Molecular Gastroenterology and Hepatology, 2022
Background & Aims: Refractory celiac disease type II (RCDII) is a rare indolent lymphoma in the small intestine characterized by a clonally expanded intraepithelial intracellular CD3+surfaceCD3-CD7+CD56- aberrant cell population.
Tessa Dieckman, Mette Schreurs, Ahmed Mahfouz, Yvonne Kooy-Winkelaar, Andra Neefjes-Borst, Gerd Bouma, Frits Koning   +6 more
doaj   +1 more source

YOUPI: Your powerful and intelligent tool for segmenting cells from imaging mass cytometry data

Frontiers in Immunology, 2023
The recent emergence of imaging mass cytometry technology has led to the generation of an increasing amount of high-dimensional data and, with it, the need for suitable performant bioinformatics tools dedicated to specific multiparametric studies.
Yvonne Scuiller, Patrice Hemon, Marion Le Rochais, Jacques-Olivier Pers, Jacques-Olivier Pers, Christophe Jamin, Christophe Jamin, Nathan Foulquier   +7 more
doaj   +1 more source

Advances in mass cytometry and its applicability to digital pathology in clinical-translational cancer research

Advances in Laboratory Medicine, 2021
The development and subsequent adaptation of mass cytometry for the histological analysis of tissue sections has allowed the simultaneous spatial characterization of multiple components.
Cereceda Karina, Jorquera Roddy, Villarroel-Espíndola Franz   +2 more
doaj   +1 more source

Imaging Mass Cytometry Antibody Staining v1

, 2020
The following SOP describes procedures required for the antibody staining of formalin fixed, paraffin-embedded (FFPE) samples. In this SOP, the term “samples” refers to FFPE tissue samples.
Michelle Daniel, Marda Jorgensen
openaire   +1 more source

Maxpar Antibody Labeling for Imaging Mass Cytometry v1 [PDF]

, 2020
Version 11 of the antibody labelling protocol published by Fluidigm.
Marda Jorgensen, Michelle Daniel
openaire   +1 more source

Single-cell mass cytometry in immunological skin diseases

Frontiers in Immunology
Immune-related skin diseases represent a collective of dermatological disorders intricately linked to dysfunctional immune system processes. These conditions are primarily characterized by an immoderate activation of the immune system or deviant immune ...
Mingming Zhao, Mingming Zhao, Mingming Zhao, Mingming Zhao, Yuqi Cheng, Yuqi Cheng, Yuqi Cheng, Yuqi Cheng, Jinping Gao, Jinping Gao, Jinping Gao, Jinping Gao, Fusheng Zhou, Fusheng Zhou, Fusheng Zhou, Fusheng Zhou   +15 more
doaj   +1 more source

Multiplexed imaging of immune cells in staged multiple sclerosis lesions by mass cytometry

eLife, 2019
Multiple sclerosis (MS) is characterized by demyelinated and inflammatory lesions in the brain and spinal cord that are highly variable in terms of cellular content.
Valeria Ramaglia, Salma Sheikh-Mohamed, Karen Legg, Calvin Park, Olga L Rojas, Stephanie Zandee, Fred Fu, Olga Ornatsky, Eric C Swanson, David Pitt, Alexandre Prat, Trevor D McKee, Jennifer L Gommerman   +12 more
doaj   +1 more source

Imaging Mass Cytometry Compensation Slide Preparation v1 [PDF]

, 2020
This SOP describes the preparation of compensation slides with single antibodyconjugate spots that can be used to estimate mass channel spillover for IMC. Spillover varies from metal lot to lot due to varying impurities. Once a spillover matrix for a specific set of antibody-conjugates has been generated, the experiment of interest, in which the same ...
Michelle Daniel, Marda Jorgensen
openaire   +1 more source

Multiplex Stimulated Raman Scattering Imaging Cytometry Reveals Lipid-Rich Protrusions in Cancer Cells under Stress Condition

iScience, 2020
Summary: In situ measurement of cellular metabolites is still a challenge in biology. Conventional methods, such as mass spectrometry or fluorescence microscopy, would either destroy the sample or introduce strong perturbations to target molecules. Here,
Kai-Chih Huang, Junjie Li, Chi Zhang, Yuying Tan, Ji-Xin Cheng   +4 more
doaj   +1 more source

Multiplexed imaging mass cytometry reveals distinct tumor-immune microenvironments linked to immunotherapy responses in melanoma

Communications Medicine, 2022
Xiao, Guo et al. use imaging mass cytometry to evaluate the spatial composition of the tumor microenvironment in melanoma. The authors identify features of the microenvironment associated with response to anti-PD-1 immunotherapy.
Xu Xiao, Qian Guo, Chuanliang Cui, Yating Lin, Lei Zhang, Xin Ding, Qiyuan Li, Minshu Wang, Wenxian Yang, Yan Kong, Rongshan Yu   +10 more
doaj   +1 more source