Results 31 to 40 of about 149,839 (140)

A 34-Marker Panel for Imaging Mass Cytometric Analysis of Human Snap-Frozen Tissue

Frontiers in Immunology, 2020
Imaging mass cytometry (IMC) is able to quantify the expression of dozens of markers at sub-cellular resolution on a single tissue section by combining a novel laser ablation system with mass cytometry.
Nannan Guo, Vincent van Unen, Vincent van Unen, Marieke E. Ijsselsteijn, Laura F. Ouboter, Andrea E. van der Meulen, Susana M. Chuva de Sousa Lopes, Noel F. C. C. de Miranda, Frits Koning, Na Li, Na Li   +10 more
doaj   +1 more source

Graph of graphs analysis for multiplexed data with application to imaging mass cytometry.

PLoS Computational Biology, 2021
Imaging Mass Cytometry (IMC) combines laser ablation and mass spectrometry to quantitate metal-conjugated primary antibodies incubated in intact tumor tissue slides.
Ya-Wei Eileen Lin, Tal Shnitzer, Ronen Talmon, Franz Villarroel-Espindola, Shruti Desai, Kurt Schalper, Yuval Kluger   +6 more
doaj   +1 more source

OPTIMAL: An OPTimized Imaging Mass cytometry AnaLysis framework for benchmarking segmentation and data exploration. [PDF]

Cytometry A
Hunter B, Nicorescu I, Foster E, McDonald D, Hulme G, Fuller A, Thomson A, Goldsborough T, Hilkens CMU, Majo J, Milross L, Fisher A, Bankhead P, Wills J, Rees P, Filby A, Merces G.   +16 more
europepmc   +2 more sources

Highly multiplexed 2-dimensional imaging mass cytometry analysis of HBV-infected liver

JCI Insight, 2021
Studies of human hepatitis B virus (HBV) immune pathogenesis are hampered by limited access to liver tissues and technologies for detailed analyses. Here, utilizing imaging mass cytometry (IMC) to simultaneously detect 30 immune, viral, and structural ...
Daniel Traum, Yue J. Wang, Kathleen B. Schwarz, Jonathan Schug, David K.H. Wong, Harry L.A. Janssen, Norah A. Terrault, Mandana Khalili, Abdus S. Wahed, Karen F. Murray, Phillip Rosenthal, Simon C. Ling, Norberto Rodriguez-Baez, Richard K. Sterling, Daryl T.Y. Lau, Timothy M. Block, Michael D. Feldman, Elizabeth E. Furth, William M. Lee, David E. Kleiner, Anna S. Lok, Klaus H. Kaestner, Kyong-Mi Chang   +22 more
doaj   +1 more source

Using Imaging Mass Cytometry to Define Cell Identities and Interactions in Human Tissues

Frontiers in Physiology, 2021
In the evolving landscape of highly multiplexed imaging techniques that can be applied to study complex cellular microenvironments, this review characterizes the use of imaging mass cytometry (IMC) to study the human kidney.
Vijayakumar R. Kakade, Marlene Weiss, Lloyd G. Cantley   +2 more
doaj   +1 more source

Comparison of Osteosarcoma Aggregated Tumour Models with Human Tissue by Multimodal Mass Spectrometry Imaging

Metabolites, 2021
Osteosarcoma (OS) is the most common primary bone malignancy and largely effects adolescents and young adults, with 60% of patients under the age of 25. There are multiple cell models of OS described in vitro that express the specific genetic alterations
Lucy E. Flint, Gregory Hamm, Joseph D. Ready, Stephanie Ling, Catherine J. Duckett, Neil A. Cross, Laura M. Cole, David P. Smith, Richard J. A. Goodwin, Malcolm R. Clench   +9 more
doaj   +1 more source

Adaptation of Imaging Mass Cytometry to Explore the Single Cell Alloimmune Landscape of Liver Transplant Rejection

Frontiers in Immunology, 2022
Rejection continues to be an important cause of graft loss in solid organ transplantation, but deep exploration of intragraft alloimmunity has been limited by the scarcity of clinical biopsy specimens.
Nolan Ung, Cameron Goldbeck, Cameron Goldbeck, Cassandra Man, Cassandra Man, Julianne Hoeflich, Julianne Hoeflich, Ren Sun, Arianna Barbetta, Arianna Barbetta, Naim Matasci, Jonathan Katz, Jerry S. H. Lee, Jerry S. H. Lee, Jerry S. H. Lee, Shefali Chopra, Shefali Chopra, Shahab Asgharzadeh, Shahab Asgharzadeh, Mika Warren, Mika Warren, Linda Sher, Linda Sher, Rohit Kohli, Rohit Kohli, Omid Akbari, Omid Akbari, Yuri Genyk, Yuri Genyk, Juliet Emamaullee, Juliet Emamaullee, Juliet Emamaullee   +31 more
doaj   +1 more source

Metal Isotope-Tagged Nanomaterials for Mass Cytometry and Imaging: Advances and Biomedical Applications

Nano Biomedicine and Engineering
Mass cytometry (cytometry by time-of-flight (CyTOF)) and imaging mass cytometry (IMC) are transformative technologies that combine flow cytometry principles with time-of-flight mass spectrometry (TOF-MS).
Dongling Jia, Minhui Cui, Weiwei Wang, Jielin Sun, Xiaoxiang Chen   +4 more
doaj   +1 more source

Imaging Mass Cytometry Data Acquisition v1 [PDF]

, 2020
This SOP describes the image acquisition with an Imaging Mass Cytometer (IMC or Hyperion). Prior to this protocol the IMC needs to be fully tuned to specifications (see “HuBMAP_Protocol_4_IMC_Tuning.pdf”). Additionally, every batch of samples stained with the same antibody-mix requires the acquisition of a compensation slide for spill over correction ...
Michelle Daniel, Marda Jorgensen
openaire   +1 more source

Novel Techniques and Future Perspective for Investigating Critical-Size Bone Defects

Bioengineering, 2022
A critical-size bone defect is a challenging clinical problem in which a gap between bone ends will not heal and will become a nonunion. The current treatment is to harvest and transplant an autologous bone graft to facilitate bone bridging.
Elijah Ejun Huang, Ning Zhang, Huaishuang Shen, Xueping Li, Masahiro Maruyama, Takeshi Utsunomiya, Qi Gao, Roberto A. Guzman, Stuart B. Goodman   +8 more
doaj   +1 more source