Results 11 to 20 of about 141,877 (234)

High-resolution imaging mass cytometry to map subcellular structures. [PDF]

Nat Methods
Imaging mass cytometry (IMC) is a powerful multiplexed imaging technology used to investigate cell phenotypes and spatial organization of tissue in health and disease. The spatial resolution of IMC is presently at 1 µm, enabling the resolution of single cells and large subcellular compartments but not submicrometer intracellular structures.
Bollhagen A, Whipman J, Coelho R, Heinzelmann-Schwarz V, Jacob F, Bodenmiller B.   +5 more
europepmc   +3 more sources

Dual-modality imaging of immunofluorescence and imaging mass cytometry for whole-slide imaging and accurate segmentation. [PDF]

Cell Rep Methods, 2023
Imaging mass cytometry (IMC) is a powerful technique capable of detecting over 30 markers on a single slide. It has been increasingly used for single-cell-based spatial phenotyping in a wide range of samples. However, it only acquires a rectangle field of view (FOV) with a relatively small size and low image resolution, which hinders downstream ...
Kim EN, Chen PZ, Bressan D, Tripathi M, Miremadi A, di Pietro M, Coussens LM, Hannon GJ, Fitzgerald RC, Zhuang L, Chang YH.   +10 more
europepmc   +4 more sources

A Map of Human Type 1 Diabetes Progression by Imaging Mass Cytometry

Cell Metabolism, 2019
Data related to the publication: "Imaging Mass Cytometry Analysis Sheds Light on Type 1 Diabetes Progression" (manuscript submitted to Cell Metabolism).
Nicolas Damond, Stefanie Engler, Vito R T Zanotelli   +2 more
exaly   +2 more sources

Highly multiplexed imaging of tumor tissues with subcellular resolution by mass cytometry

Nature Methods, 2014
Mass cytometry enables high-dimensional, single-cell analysis of cell type and state. In mass cytometry, rare earth metals are used as reporters on antibodies.
Hao A O Wang, Denis Schapiro, Nevena Zivanovic   +2 more
exaly   +2 more sources

MATISSE: a method for improved single cell segmentation in imaging mass cytometry [PDF]

BMC Biology, 2021
Background Visualizing and quantifying cellular heterogeneity is of central importance to study tissue complexity, development and physiology, and has a vital role in understanding pathologies.
Matthijs J.D Baars, Laclé, Miangela M, Stephanie van Dam, Maroussia M.P. Ganpat, Mojtaba Amini, Sinha, Neeraj, Miangela M. Laclé, Baars, Matthijs J D, van Dam, Stephanie, Annelies Pieterman-Bos, Yvonne Vercoulen, Neeraj Sinha, Oldenburg, Bas, Bas Oldenburg, Amini, Mojtaba, Ganpat, Maroussia M P, Vercoulen, Yvonne, Pieterman-Bos, Annelies   +17 more
core   +5 more sources

Integration of mass cytometry and mass spectrometry imaging for spatially resolved single-cell metabolic profiling

Nature Methods
The integration of spatial omics technologies can provide important insights into the biology of tissues. Here we combined mass spectrometry imaging-based metabolomics and imaging mass cytometry-based immunophenotyping on a single tissue section to ...
Joana B Nunes, Marieke E Ijsselsteijn, Rick Ursem   +2 more
exaly   +3 more sources

Methods for analyzing tellurium imaging mass cytometry data.

PLOS ONE, 2019
Imaging mass cytometry (IMC) is a technique allowing visualization and quantification of over 40 biological parameters in a single experiment with subcellular spatial resolution, however most IMC experiments are limited to endpoint analysis with ...
Jay Bassan, Mark Nitz
core   +5 more sources

Enhancing Mass-Cytometry Imaging Analysis

Inquiry@Queen's Undergraduate Research Conference Proceedings
Mass cytometry imaging is a technique that utilizes mass spectrometry instead of fluorescent flow cytometry to detect metal-conjugated antibodies binding to single cell antigens.
Tuitt-Barnes, Dream
core   +2 more sources

Channel crosstalk correction in suspension and imaging mass cytometry [PDF]

, 2017
Mass cytometry enables simultaneous analysis of over 40 proteins and their modifications in single cells through use of metal-tagged antibodies. Compared to fluorescent dyes, the use of pure metal isotopes strongly reduces spectral overlap among ...
Stéphane Chevrier, Mark D. Robinson, Helena Crowell, Bernd Bodenmiller, Vito Zanotelli, Stefanie Engler   +5 more
core   +2 more sources

Multiplexed Imaging Mass Cytometry Analysis in Preclinical Models of Pancreatic Cancer. [PDF]

Int J Mol Sci
Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal cancers. PDAC is characterized by a complex tumor microenvironment (TME), that plays a pivotal role in disease progression and resistance to therapy. Investigating the spatial distribution and interaction of TME cells with the tumor is the basis for understanding the mechanisms ...
Erreni M, Fumagalli MR, Zanini D, Candiello E, Tiberi G, Parente R, D'Anna R, Magrini E, Marchesi F, Cappello P, Doni A.   +10 more
europepmc   +3 more sources