Results 51 to 60 of about 5,255,854 (171)

Multiplexed imaging of immune cells in staged multiple sclerosis lesions by mass cytometry

eLife, 2019
Multiple sclerosis (MS) is characterized by demyelinated and inflammatory lesions in the brain and spinal cord that are highly variable in terms of cellular content.
Valeria Ramaglia, Salma Sheikh-Mohamed, Karen Legg, Calvin Park, Olga L Rojas, Stephanie Zandee, Fred Fu, Olga Ornatsky, Eric C Swanson, David Pitt, Alexandre Prat, Trevor D McKee, Jennifer L Gommerman   +12 more
doaj   +1 more source

Organoid Coculture Models for Cancer Research and Immunotherapy

Med Research, EarlyView.
This review summarizes organoid coculture platforms, including submerged Matrigel culture, air–liquid interface, microfluidic/organoid‐on‐a‐chip, and 3D bioprinting, for reconstructing the tumor microenvironment to study tumor–stroma/immune crosstalk, evaluate immunotherapies, and enable drug screening.
Zhuo Yang, Huihui Yang, Guangxin Li, Guobang Liu, Zhulan Chen, Wenzhi Tian, Meiyang Huang, Xiaoling Liu, Yongsheng Zhao, Dong Chen   +9 more
wiley   +1 more source

Lupus Panniculitis Immunoprofiling Reveals a Cytotoxic, Type 1‐Skewed T‐Cell Response With Signs of Metabolic Alteration

Allergy, EarlyView.
Imaging mass cytometry shows lupus panniculitis lesions are dominated by cytotoxic and skin‐homing T cells with abundant macrophages and B‐cell aggregates. Spatial neighborhood analysis reveals organized leukocyte architecture, including B‐cell proximity to naïve T cells and M1 macrophages to cytotoxic T cells.
Milad Ameri, Reihane Ziadlou, Stephan Traidl, Milena Sokolowska, Emmanuel Contassot, Nick Li, Maija Kiuru, Isabel Kolm, Werner Kempf, Emanual Maverakis, Cezmi Akdis, Marie‐Charlotte Brüggen   +11 more
wiley   +1 more source

Spatial Transcriptomics Identifies Characteristic Immunological Niches in Atopic Dermatitis

Allergy, EarlyView.
Spatially organized immune niches containing mature migratory dendritic cells (mmDCs) and CD4+ T cells in atopic dermatitis lesions support localized T cell activation and may contribute to disease persistence. Spatial transcriptomics identifies conserved immune niches in atopic dermatitis lesions, characterized by close co‐localization of CD4+ T cells
Lina Gross, Lukas Bäriswyl, Erik Kupschke, Alexandra Wallimann, Hanne Hillen, Philipp Kirchner, Jivko Kamarashev, Martin Wartenberg, Claudio Rhyner, Christoph Schlapbach, Stephan Traidl, Mirjam Schenk   +11 more
wiley   +1 more source

Imaging mass cytometric analysis of human fetal intestinal samples

, 2022
To explore the development and spatial organization of the fetal intestinal immune system in situ, we employed imaging mass cytometry (IMC) on tissue sections of human fetal intestinal samples from different gestational ages.THIS DATASET IS ARCHIVED AT ...
Guo, N (via Mendeley Data), Guo, Nannan
core   +1 more source

Coupling imaging mass cytometry with Alcian blue histochemical staining for a single-slide approach

Frontiers in Immunology
Imaging mass cytometry (IMC) is a metal mass spectrometry-based method allowing highly multiplex immunophenotyping of cells within tissue samples. However, some limitations of IMC are its 1-µm resolution and its time and costs of analysis limiting ...
Patrice Hemon, Danivanh Ben-Guigui, Margaux Geier, Margaux Geier, Marine Castillon, Marine Castillon, Corentin Paranthoen, Corentin Paranthoen, Jacques-Olivier Pers, Marion Le Rochais, Arnaud Uguen, Arnaud Uguen   +11 more
doaj   +1 more source

High‐Intensity Interval Training Modulates NLRP3 Inflammasome‐Related Expression and Monocyte Subset Distribution in Adults With Obesity: A Randomised Trial

Immunology, EarlyView.
In sedentary adults with obesity, 8 weeks of high‐intensity interval training prevented the shift toward a pro‐inflammatory monocyte profile seen in nontrained controls—most robustly, a significantly lower proportion of intermediate monocytes at Week 8 (p = 0.014) while the control group also showed a within‐group rise in NLRP3 expression not observed ...
Daniela A. de Abreu, Ana Luíza P. A. Silveira, Stella de S. Vieira, Francisco A. H. Fonseca, Maria Cristina Izar, Jônatas B. do Amaral, Carolina N. França   +6 more
wiley   +1 more source

Characterization of papillary and clear cell renal cell carcinoma through imaging mass cytometry reveals distinct immunologic profiles

Frontiers in Immunology, 2023
ObjectiveTo characterize and further compare the immune cell populations of the tumor microenvironment (TME) in both clear cell and papillary renal cell carcinoma (RCC) using heavy metal-labeled antibodies in a multiplexed imaging approach (imaging mass ...
Ameish Govindarajan, Nicholas J. Salgia, Nicholas J. Salgia, Nicholas J. Salgia, Haiqing Li, Daniela V. Castro, Tamara Mirzapoiazova, Brian Armstrong, Dan Zhao, Dan Zhao, Benjamin D. Mercier, Nazli Dizman, Nazli Dizman, Neal Chawla, Zeynep Zengin, Luis Meza, Nishita Tripathi, Nicolas Sayegh, Alex Chehrazi-Raffle, Abhishek Tripathi, Sumanta K. Pal   +20 more
doaj   +1 more source

Immunomodulatory Peptide LW‐9 Enhances Doxorubicin Efficacy by Downregulating PD‐L1 and Promoting Intratumoral T‐Cell Responses in Murine Breast Cancer

Immunology, EarlyView.
LW‐9, a peptide derived from Phoneutria nigriventer venom, enhances antitumor immunity in a murine breast cancer model by remodelling the tumour microenvironment. Treatment reduces PD‐L1 expression while increasing CD3+ and GITR+ T‐cell infiltration, resulting in delayed tumour progression, particularly when combined with doxorubicin.
Ingrid Mayara Cavalcante Trevisan, Cibele Pereira Mariano, Sabrina Carvalho Brunelli, Gabriel da Silva Cordeiro, Jessika Takaki, Valquiria Aparecida Matheus, Liana Verinaud, Catarina Rapôso   +7 more
wiley   +1 more source

Deubiquitinase USP5 suppresses ferroptosis in lung adenocarcinoma by deubiquitinating and stabilizing GPX3

Journal of Cell Communication and Signaling, Volume 20, Issue 4, December 2026.
1. USP5 overexpression in LUAD predicts poor prognosis, linking to tumor progression and therapy resistance. 2. USP5 deubiquitinates/stabilizes GPX3, maintaining antioxidant defense against ferroptosis. 3. Targeting inhibition of USP5 enhances LUAD ferroptosis sensitivity, offering therapeutic potential. Abstract Ferroptosis is a regulated form of cell
Xin Fan, Yubiao Lin, Zhigao Zhen, Yingqin Gao, Wenhui Zhen, Siyuan Zeng, Yufei Ji, Kaida Huang, Shuitu Feng   +8 more
wiley   +1 more source