Results 11 to 20 of about 5,255,854 (171)

MATISSE: a method for improved single cell segmentation in imaging mass cytometry [PDF]

BMC Biology, 2021
Background Visualizing and quantifying cellular heterogeneity is of central importance to study tissue complexity, development, and physiology and has a vital role in understanding pathologies.
Matthijs J. D. Baars, Neeraj Sinha, Mojtaba Amini, Annelies Pieterman-Bos, Stephanie van Dam, Maroussia M. P. Ganpat, Miangela M. Laclé, Bas Oldenburg, Yvonne Vercoulen   +8 more
doaj   +2 more sources

MATISSE: An analysis protocol for combining imaging mass cytometry with fluorescence microscopy to generate single-cell data [PDF]

STAR Protocols, 2022
Summary: Exploring tissue heterogeneity on a single-cell level by imaging mass cytometry (IMC) remains challenging because of its limiting resolution. We previously demonstrated that combining higher resolution fluorescence with IMC data in the analysis ...
Daniëlle Krijgsman, Neeraj Sinha, Matthijs J.D. Baars, Stephanie van Dam, Mojtaba Amini, Yvonne Vercoulen   +5 more
doaj   +2 more sources

Methods for analyzing tellurium imaging mass cytometry data.

PLoS ONE, 2019
Imaging mass cytometry (IMC) is a technique allowing visualization and quantification of over 40 biological parameters in a single experiment with subcellular spatial resolution, however most IMC experiments are limited to endpoint analysis with ...
Jay Bassan, Mark Nitz
doaj   +2 more sources

Advances in mass cytometry and its applicability to digital pathology in clinical-translational cancer research

Advances in Laboratory Medicine, 2021
The development and subsequent adaptation of mass cytometry for the histological analysis of tissue sections has allowed the simultaneous spatial characterization of multiple components.
Cereceda Karina, Jorquera Roddy, Villarroel-Espíndola Franz   +2 more
doaj   +2 more sources

High Dimensional Imaging Mass Cytometry Panel to Visualize the Tumor Immune Microenvironment Contexture

Frontiers in Immunology, 2021
The integrative analysis of tumor immune microenvironment (TiME) components, their interactions and their microanatomical distribution is mandatory to better understand tumor progression.
Roxane Elaldi, Roxane Elaldi, Patrice Hemon, Luciana Petti, Estelle Cosson, Belinda Desrues, Anne Sudaka, Gilles Poissonnet, Ellen Van Obberghen-Schilling, Jacques-Olivier Pers, Veronique M. Braud, Fabienne Anjuère, Aïda Meghraoui-Kheddar   +12 more
doaj   +2 more sources

Development of 42 marker panel for in-depth study of cancer associated fibroblast niches in breast cancer using imaging mass cytometry

Frontiers in Immunology
Imaging Mass Cytometry (IMC) is a novel, and formidable high multiplexing imaging method emerging as a promising tool for in-depth studying of tissue architecture and intercellular communications. Several studies have reported various IMC antibody panels
Hanna Røgenes, Kenneth Finne, Ingeborg Winge, Lars A. Akslen, Lars A. Akslen, Arne Östman, Arne Östman, Vladan Milosevic   +7 more
doaj   +4 more sources

Different approaches to Imaging Mass Cytometry data analysis [PDF]

, 2023
Imaging Mass Cytometry (IMC) is a novel, high multiplexing imaging platform capable of simultaneously detecting and visualizing up to 40 different protein targets.
Milosevic, Vladan
core   +1 more source

Multi‐Omics Integration Reveals Metabolic Reprogramming and EMT‐Driven Metastasis in Triple‐Negative Breast Cancer [PDF]

Cancer Sci
Integrated transcriptomic, metabolomic, and spatial proteomic profiling of TNBC tumors identifies a metastatic‐associated molecular landscape characterized by EMT activation, metabolic reprogramming, and tumor microenvironment (TME) remodeling. Multi‐omics integration reveals coordinated alterations in EMT signatures, amino acid metabolism, immune ...
Pang Y, Shang Y, Wang W, Liu T, Zou X.
europepmc   +2 more sources

Using Imaging Mass Cytometry to Define Cell Identities and Interactions in Human Tissues

Frontiers in Physiology, 2021
In the evolving landscape of highly multiplexed imaging techniques that can be applied to study complex cellular microenvironments, this review characterizes the use of imaging mass cytometry (IMC) to study the human kidney.
Vijayakumar R. Kakade, Marlene Weiss, Lloyd G. Cantley   +2 more
doaj   +1 more source

Mass Cytometric Analysis of Early-Stage Mycosis Fungoides

Cells, 2022
Mycosis fungoides (MF) is the most common subtype of cutaneous T-cell lymphoma. Early-stage disease is characterized by superficial infiltrates of small- to medium-sized atypical epidermotropic T lymphocytes that are clonal related.
Nannan Guo, Li Jia, Coby Out-Luiting, Noel F. C. C. de Miranda, Rein Willemze, Frits Koning, Maarten Vermeer, Koen Quint   +7 more
doaj   +1 more source