Results 51 to 60 of about 195,947 (313)

Fluorescence Correlation Spectroscopy Reveals Efficient Cytosolic Delivery of Protein Cargo by Cell-Permeant Miniature Proteins. [PDF]

, 2018
New methods for delivering proteins into the cytosol of mammalian cells are being reported at a rapid pace. Differentiating between these methods in a quantitative manner is difficult, however, as most assays for evaluating cytosolic protein delivery are
Knox, Susan L, Schepartz, Alanna, Steinauer, Angela, Thompson, Alexander D, Wissner, Rebecca F   +4 more
core   +2 more sources

The Second Transmembrane Domain of P2X7 Contributes to Dilated Pore Formation [PDF]

, 2013
Activation of the purinergic receptor P2X7 leads to the cellular permeability of low molecular weight cations. To determine which domains of P2X7 are necessary for this permeability, we exchanged either the C-terminus or portions of the second ...
A MacKenzie, A Surprenant, AN Shemon, B Gu, BC Suh, BJ Gu, BJ Gu, BM Saunders, BS Khakh, BS Khakh, C Andrei, C Marques-da-Silva, C Sun, C Virginio, Chengqun Sun, CP Lai, D Leon, D Zink, DS Emmett, DS Samways, E Adinolfi, E Bulanova, F Bretschneider, F Rassendren, G Girolomoni, H Gunosewoyo, HJ Bradley, HM Costa-Junior, HZ Hu, JS Wiley, K Schroder, KA Hillman, L Gudipaty, L Stokes, LC Denlinger, LC Denlinger, LE Browne, LH Jiang, LH Jiang, M Li, M Li, Michelle E. Heid, ML Gavala, ML Smart, MT Miras-Portugal, P Marin-Garcia, P Pelegrin, P Pelegrin, Peter A. Keyel, Pierre Bobé, Q Li, R Sluyter, RA North, Russell D. Salter, S Chaumont, S Kracun, S Locovei, S Penuela, S Roger, SD Ohlendorff, SD Silberberg, SL Fernando, SL Fernando, T Kawate, T Sugiyama, TM Egan, V Petrilli, W Duckwitz, Y Shinozaki   +68 more
core   +6 more sources

Imaging Mass Cytometry Compensation Slide Preparation v1 [PDF]

, 2020
This SOP describes the preparation of compensation slides with single antibodyconjugate spots that can be used to estimate mass channel spillover for IMC. Spillover varies from metal lot to lot due to varying impurities. Once a spillover matrix for a specific set of antibody-conjugates has been generated, the experiment of interest, in which the same ...
Michelle Daniel, Marda Jorgensen
openaire   +1 more source

Comparison of Osteosarcoma Aggregated Tumour Models with Human Tissue by Multimodal Mass Spectrometry Imaging

Metabolites, 2021
Osteosarcoma (OS) is the most common primary bone malignancy and largely effects adolescents and young adults, with 60% of patients under the age of 25. There are multiple cell models of OS described in vitro that express the specific genetic alterations
Lucy E. Flint, Gregory Hamm, Joseph D. Ready, Stephanie Ling, Catherine J. Duckett, Neil A. Cross, Laura M. Cole, David P. Smith, Richard J. A. Goodwin, Malcolm R. Clench   +9 more
doaj   +1 more source

Engineered Tumor-Targeted T Cells Mediate Enhanced Anti-Tumor Efficacy Both Directly and through Activation of the Endogenous Immune System. [PDF]

, 2018
Chimeric antigen receptor (CAR) T cell therapy has proven clinically beneficial against B cell acute lymphoblastic leukemia and non-Hodgkin's lymphoma. However, suboptimal clinical outcomes have been associated with decreased expansion and persistence of
Avanzi, Mauro P, Brentjens, Renier J, Cheung, Kenneth, Daniyan, Anthony F, Li, Xinghuo, Park, Hyebin, Purdon, Terence J, Spitzer, Matthew H, van Leeuwen, Dayenne G, Wijewarnasuriya, Dinali P, Yeku, Oladapo   +10 more
core   +1 more source

3D printing for bio-synthetic biliary stents [PDF]

, 2019
Three-dimensional (3D) printing is an additive manufacturing method that holds great potential in a variety of future patient-specific medical technologies.
Alexander, Jonathan Steven, Ballard, David, Boktor, Moheb, Boktor, Moheb, Boyer, Christen J., Ghali, G.E., Huebert, Robert, Samant, Hrishikesh, Wang, Yuping, White, Luke, Woerner, Jennifer E.   +10 more
core   +2 more sources

Compensation of Signal Spillover in Suspension and Imaging Mass Cytometry [PDF]

Cell Systems, 2018
ISSN:2405 ...
Chevrier, Stéphane, Crowell, Helena L, Zanotelli, Vito R T, Engler, Stefanie, Robinson, Mark D, Bodenmiller, Bernd   +5 more
openaire   +5 more sources

The effects of aggregation and protein corona on the cellular internalization of iron oxide nanoparticles

, 2011
Engineered inorganic nanoparticles are essential components in the development of nanotechnologies. For applications in nanomedicine, particles need to be functionalized to ensure a good dispersibility in biological fluids.
Berret, J. -F., Conjeaud, H., Courtois, J., Safi, M., Seigneuret, M.   +4 more
core   +2 more sources

Altered brain energetics induces mitochondrial fission arrest in Alzheimer's Disease. [PDF]

, 2016
Altered brain metabolism is associated with progression of Alzheimer's Disease (AD). Mitochondria respond to bioenergetic changes by continuous fission and fusion.
Bachmeier, Benjamin V, Christensen, Trace A, Diaz Brinton, Roberta, Gateno, Benjamin, Gylys, Karen H, Itoh, Kie, Parisi, Joseph E, Patterson, Emily R, Poon, Wayne W, Salisbury, Jeffrey L, Schroeder, Andreas, Sesaki, Hiromi, Trushin, Sergey, Trushina, Eugenia, Yao, Jia, Zhang, Liang   +15 more
core   +1 more source

Novel Techniques and Future Perspective for Investigating Critical-Size Bone Defects

Bioengineering, 2022
A critical-size bone defect is a challenging clinical problem in which a gap between bone ends will not heal and will become a nonunion. The current treatment is to harvest and transplant an autologous bone graft to facilitate bone bridging.
Elijah Ejun Huang, Ning Zhang, Huaishuang Shen, Xueping Li, Masahiro Maruyama, Takeshi Utsunomiya, Qi Gao, Roberto A. Guzman, Stuart B. Goodman   +8 more
doaj   +1 more source