Results 131 to 140 of about 322,348 (369)

Pitfall in the high-throughput quantification of whole blood cyclosporin A using liquid chromatography-tandem mass spectrometry [PDF]

open access: yes, 2005
In a growing number of laboratories the technique of liquid chromatography-tandem mass spectrometry is used for the quantification of cyclosporin A in whole blood, employing cyclosporin D as the internal standard. Cyclosporin A is extensively metabolized
Spöhrer, Ute, Vogeser, Michael
core   +1 more source

A Smartphone Controlled Handheld Microfluidic Liquid Handling System [PDF]

open access: yesarXiv, 2014
Microfluidics and lab-on-a-chip technologies have made it possible to manipulate small volume liquids with unprecedented resolution, automation and integration. However, most current microfluidic systems still rely on bulky off-chip infrastructures such as compressed pressure sources, syringe pumps and computers to achieve complex liquid manipulation ...
arxiv  

DNA‐PKcs‐Driven YAP1 Phosphorylation and Nuclear Translocation: a Key Regulator of Ferroptosis in Hyperglycemia‐Induced Cardiac Dysfunction in Type 1 Diabetes

open access: yesAdvanced Science, EarlyView.
In the context of chronic hyperglycemia, a DDR is initiated, leading to the pathological activation of DNA‐PKcs in the diabetic heart. This activated DNA‐PKcs directly interacts with and phosphorylates YAP1 at Thr226, thereby increasing the nuclear expression of YAP1.
Junyan Wang   +10 more
wiley   +1 more source

Nucleic Acid Lateral Flow Immunoassay for the Detection of Pathogenic Bacteria from Food [PDF]

open access: yes, 2009
Nucleic acid lateral flow immunoassay (NALFIA) is a method combining molecular biological principle of detection with immunochemical principle of visualisation.
Amerongen, A., van   +5 more
core   +2 more sources

Analysis of Toxigenic Fusarium Species Associated with Wheat Grain from Three Regions of Russia: Volga, Ural, and West Siberia

open access: yesToxins, 2019
Wheat grains collected in three regions of Russia—Volga, Ural, and West Siberia—were analyzed for triangulation of methods in analysis of toxigenic Fusarium species.
Tatiana Gagkaeva   +6 more
doaj   +1 more source

Two-orders of magnitude improvement detection limit of lateral flow assays using isotachophoresis [PDF]

open access: yesarXiv, 2014
Lateral flow (LF) immunoassays are one of the most prevalent point-of-care (POC) diagnostics due to their simplicity, low cost, and robust operation. A common criticism of LF tests is that they have poor detection limits compared to analytical techniques, like ELISA, which confines their application as a diagnostic tool.
arxiv  

Enzyme immunoassay (EIA)/enzyme-linked immunosorbent assay (ELISA).

open access: yesClinical Chemistry, 2005
This brief note addresses the historical background of the invention of the enzyme immunoassay (EIA) and enzyme-linked immunosorbent assay (ELISA). These assays were developed independently and simultaneously by the research group of Peter Perlmann and ...
R. Lequin
semanticscholar   +1 more source

Nuclear Translocation of S100A9 Triggers Senescence of Human Amnion Fibroblasts by De‐Repressing LINE1 Via Heterochromatin Erosion at Parturition

open access: yesAdvanced Science, EarlyView.
This study shows that the classical secretory protein S100 calcium‐binding protein A9 (S100A9) can translocate to the nucleus upon de‐phosphorylation at Thr 113 in human amnion fibroblasts at parturition, where S100A9 induces heterochromatin erosion through segregation of the heterochromatin maintenance protein, resulting inLong Interspersed Nuclear ...
Fan Zhang   +8 more
wiley   +1 more source

Heterologous screening of hybridomas for the development of broad-specific monoclonal antibodies against deoxynivalenol and its analogues [PDF]

open access: yes, 2014
Hapten heterology was introduced into the steps of hybridoma selection for the development of monoclonal antibodies (MAbs) against deoxynivalenol (DON).
Bracke, Marc   +7 more
core   +1 more source

Ag@Au Core–Shell Porous Nanocages with Outstanding SERS Activity for Highly Sensitive SERS Immunoassay

open access: yesSensors, 2019
A highly sensitive immunoassay of biomarkers has been achieved using 4-mercaptobenzoic acid-labeled Ag@Au core–shell porous nanocage tags and α-fetoprotein immuno-sensing chips.
Yaqi Huang   +5 more
doaj   +1 more source

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