Results 51 to 60 of about 723,902 (263)

Single Cell Determination of 7,8-dihydro-8-oxo-2′-deoxyguanosine by Fluorescence Techniques: Antibody vs. Avidin Labeling

Molecules, 2023
An important biomarker of oxidative damage in cellular DNA is the formation of 7,8-dihydro-8-oxo-2′-deoxyguanosine (8-oxodG). Although several methods are available for the biochemical analysis of this molecule, its determination at the single cell level
Giusy Maraventano, Giulio Ticli, Ornella Cazzalini, Lucia A. Stivala, Mariella Ramos-Gonzalez, José-Luis Rodríguez, Ennio Prosperi   +6 more
doaj   +1 more source

KDM7A and KDM1A inhibition suppresses tumour promoting pathways in prostate cancer

Molecular Oncology, EarlyView.
Treatment resistance is a major challenge for patients with advanced prostate cancer. This study examined an alternative approach to target the major prostate cancer‐promoting pathway by targeting epigenetic factors, whose levels are higher in tumours.
Jennie N Jeyapalan, Veronika M Metzler, Simone de Brot, Corinne L Woodcock, Anna E Harris, Jennifer Lothion‐Roy, Emeli M Nilsson, Atara Ntekim, Michael S Toss, Jenny L Persson, Francesca Khani, Brian D Robinson, Lorraine J Gudas, Emad Rakha, David M Heery, Catrin S Rutland, Nigel P Mongan   +16 more
wiley   +1 more source

Developmental programmes drive cellular plasticity, disease progression and therapy resistance in lung adenocarcinoma

Molecular Oncology, EarlyView.
This study shows that lung adenocarcinomas exploit developmental branching morphogenesis to acquire a therapy resistant basal‐like tumour cell state. This process was found to be regulated by combined TP53 loss‐of‐function and type‐I interferon signalling, identifying a novel axis for biomarker and therapeutic target discovery.
Kamila J Bienkowska, Stephany Gallardo, Nur S. Zainal, Leena Arora, Matthew Ellis, Maria‐Antoinette Lopez, Judith Austine, Sai Pittla, Serena J Chee, Aiman Alzetani, Emily C Shaw, Christian H Ottensmeier, Gareth J Thomas, Christopher J Hanley   +13 more
wiley   +1 more source

FISH and immunofluorescence analysis.

, 2013
FISH with GINS2 specific BAC (pink) and simultaneous immunofluorescence staining with GFAP (green) revealed GINS2 amplification in cells with beginning GFAP expression after 5 d of differentiation (A).
Meike Voss (323248), Cornelius Welter (323251), Andreas Keller (29147), Ulrike Fischer (217763), Eckart Meese (46359), Christina Backes (34966)   +5 more
core   +1 more source

Diagnostic significance of the determination of antinuclear antibodies in children with autoimmune hepatitis

Аллергология и Иммунология в Педиатрии
.
A. A. Zhuzhula, O. V. Kurbatova, S. V. Petrichuk, D. V. Parakhina, M. A. Snovskaya, G. B. Movsisyan, E. L. Semikina, A. S. Potapov, A. P. Fisenko   +8 more
doaj   +1 more source

Ehrlichia chaffeensis Co-Opts Phagocytic Hemocytes for Systemic Dissemination in the Lone Star Tick, Amblyomma americanum

Journal of Innate Immunity, 2023
Introduction: Hematophagous arthropods can acquire and transmit several pathogens of medical importance. In ticks, the innate immune system is crucial in the outcome between vector-pathogen interaction and overall vector competence. However, the specific
Abdulsalam Adegoke, Julia Hanson, Ryan C. Smith, Shahid Karim   +3 more
doaj   +1 more source

Loss of IGF‐1R impairs DNA‐PKcs recruitment to chromatin leading to defective end‐joining

Molecular Oncology, EarlyView.
IGF‐1R promotes radioresistance by facilitating DNA‐PKcs recruitment to chromatin, enabling non‐homologous end‐joining (NHEJ) repair of double‐strand breaks. Inhibition or loss of IGF‐1R disrupts this recruitment to damage sites, driving compensatory reliance on microhomology‐mediated end‐joining (MMEJ) repair.
Matthew O. Ellis, Jack V. Mills, Wojciech Niedzwiedz, Valentine M. Macaulay   +3 more
wiley   +1 more source

Immunofluorescence staining.

, 2014
(a) Representative images of immunofluorescence staining at day 14 (100×).
Su-Hyang Kim (540723), Yun Gyeong Kang (540718), So Hee Park (540720), Ji Won Shin (540719), Jung-Woog Shin (474739), Min-Jae Oh (540721), Hyo Soon Park (540722)   +6 more
core   +1 more source

The timing of the formation and usage of replicase clusters in S-phase nuclei of human diploid fibroblasts [PDF]

, 1991
The sites of nascent DNA synthesis were compared with the distribution of the proliferating cell nuclear antigen (PCNA) in S-phase nuclei of human diploid fibroblasts (HDF) by two in vitro techniques.
Kill, IR, Campbell, KHS, Hutchison, CJ, Bridger, JM, Maldonado-Codina, G   +4 more
core   +3 more sources

Histological and Immunolabeling Techniques in Arabidopsis thaliana: A Practical Guide and Standardization Roadmap

Agronomy
Arabidopsis thaliana is a widely used model in plant biology, where histology (HT), histochemistry (HC), immunohistochemistry (IHC), and immunofluorescence (IF) are applied to study cellular structures, macromolecules, and antigens.
Samuel Valdebenito, Alexis Rubio, Alejandra Moller, Javier Santa Cruz, Priscila Castillo, Mayra Lirayén Providell, Camila Cáceres, Diego Calbucheo, Ignacia Hernández, Patricia Peñaloza   +9 more
doaj   +1 more source