Results 11 to 20 of about 24,709 (211)

Fab’ Fragment‐Immobilized Gold Surface for Capturing EpCAM‐Positive Breast Cancer Cells [PDF]

Eng Life Sci
APTES modified glass surfaces enable stable gold coating. Anti‐EpCAM Fab’ fragments can be obtained from anti‐EpCAM antibody by consecutive pepsin digestion and TCEP reduction. The free ‐SH groups on anti‐EpCAM Fab’ fragments give strong bounds with Au elements on the gold surface.
Kaga E, Kaga S, Yalcin O, Erguner G, Okumus N.   +4 more
europepmc   +2 more sources

Site-selective protein modification via disulfide rebridging for fast tetrazine/trans-cyclooctene bioconjugation [PDF]

, 2020
An inverse electron demand Diels–Alder reaction between tetrazine and trans-cyclooctene (TCO) holds great promise for protein modification and manipulation.
Chudasama, V., Kuan, S., Nogueira, J., Raabe, M., Weil, T., Xu, L., Zegota, M.   +6 more
core   +1 more source

In silico antibody engineering for SARS-CoV-2 detection

Computational and Structural Biotechnology Journal, 2021
Engineered immunoglobulin-G molecules (IgGs) are of wide interest for the development of detection elements in protein-based biosensors with clinical applications.
Didac Martí, Eduard Martín-Martínez, Juan Torras, Oscar Bertran, Pau Turon, Carlos Alemán   +5 more
doaj   +1 more source

Cell-free synthesis of functional and endotoxin-free antibody Fab fragments by translocation into microsomes

BioTechniques, 2012
A eukaryotic cell-free system based on Spodoptera frugiperda cells was developed for the convenient synthesis of Fab antibody fragments and other disulfide bridge containing proteins.
Helmut Merk, Christine Gless, Barbara Maertens, Michael Gerrits, Wolfgang Stiege   +4 more
doaj   +1 more source

Platelets and lymphocytes drive progressive penumbral tissue loss during middle cerebral artery occlusion in mice

Journal of Neuroinflammation, 2021
Background In acute ischemic stroke, cessation of blood flow causes immediate tissue necrosis within the center of the ischemic brain region accompanied by functional failure in the surrounding brain tissue designated the penumbra.
Michael K. Schuhmann, Michael Bieber, Maximilian Franke, Alexander M. Kollikowski, David Stegner, Katrin G. Heinze, Bernhard Nieswandt, Mirko Pham, Guido Stoll   +8 more
doaj   +1 more source

Binding between the neural cell adhesion molecules axonin-1 and Nr- CAM/Bravo is involved in neuron-glia interaction [PDF]

, 1995
Neural cell adhesion molecules of the immunoglobulin superfamily mediate cellular interactions via homophilic binding to identical molecules and heterophilic binding to other family members or structurally unrelated cell-surface glycoproteins.
Buchstaller, Andrea, Dreyer, William J., Giger, Roman J., Pollerberg, G. Elisabeth, Sonderegger, Peter, Suter, Daniel M.   +5 more
core   +4 more sources

Purification of the Major Outer Membrane Protein of Azospirillum brasilense, Its Affinity to Plant Roots, and Its Involvement in Cell Aggregation

Molecular Plant-Microbe Interactions, 2001
The major outer membrane protein (MOMP) of the nitrogen-fixing rhizobacterium Azospirillum brasilense strain Cd was purified and isolated by gel filtration, and antiserum against this protein was obtained.
Saul Burdman, Gabriella Dulguerova, Yaacov Okon, Edouard Jurkevitch   +3 more
doaj   +1 more source

Refinement of an ovine-based immunoglobulin therapy against SARS-CoV-2, with comparison of whole IgG versus F(ab′)2 fragments

Scientific Reports, 2023
The development of new therapies against SARS-CoV-2 is required to extend the toolkit of intervention strategies to combat the global pandemic. In this study, hyperimmune plasma from sheep immunised with whole spike SARS-CoV-2 recombinant protein has ...
Stephen Findlay-Wilson, Linda Easterbrook, Sandra Smith, Neville Pope, Matthew Aldridge, Gareth Humphries, Holger Schuhmann, Didier Ngabo, Emma Rayner, Ashley Otter, Thomas Coleman, Bethany Hicks, Rachel Halkerston, Kostis Apostolakis, Stephen Taylor, Susan Fotheringham, Amanda Horton, Irene CanoCejas, Matthew Wand, Julia A. Tree, Mark Sutton, Victoria Graham, Roger Hewson, Stuart Dowall   +23 more
doaj   +1 more source

Crystallization of immunoglobulin Fab fragments specific for DNA.

Journal of Biological Chemistry, 1988
The purification and crystallization of Fab fragments of two mouse monoclonal immunoglobulins specific for different DNA structures are described. In each case, papain digestion of the immunoglobulins produced a mixture of Fab species differing in their isoelectric points.
C. D. Mol, Amechand Boodhoo, Wayne F. Anderson, Jeremy S. Lee   +3 more
openaire   +3 more sources

Humanized CD30-Targeted Chimeric Antigen Receptor T Cells Exhibit Potent Preclinical Activity Against Hodgkin’s Lymphoma Cells

Frontiers in Cell and Developmental Biology, 2022
CD30-directed chimeric antigen receptors (CARs) with single chain antibody fragment (scFv)-binding domains from murine HRS3 show strong cytotoxicity to Hodgkin’s Lymphoma cells and have been used in clinical trials.
Jing Guo, Shuai He, Yongjie Zhu, Wei Yu, Dong Yang, Xudong Zhao   +5 more
doaj   +1 more source