Results 31 to 40 of about 50 (50)
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Dissociation and denaturation equilibriums and kinetics of a homogeneous human immunoglobulin Fab fragment

Biochemistry, 1976
The conformational equilibria and the kinetics of the approach to equilibrium of an IgG1 myeloma (Wes) Fab fragment (SSFab) and its mildly reduced and S-carboxyamidomethylated derivative (RAFab) were studied as a function of guanidine hydrochloride concentration.
openaire   +2 more sources

Structure of chicken 7S immunoglobulin allotypes: Further studies on localization to Fab and Fc fragments

Molecular Immunology, 1981
Abstract Papain-derived fragments (Fab and Fc) were used to localize previously uncharacterized chicken 7S immunoglobulin (Ig§) allotypic specificities (G-1.7, G-1.8, G-1.9, G-1.10, and G-1.12). Based on the molar amounts of Fab and Fc preparations relative to undigested 7S Ig to inhibit 50% of binding of 125 I-7S Ig with homologous alloantiserum ...
L K, Ch'ng, A A, Benedict
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An easy and simple separation method for Fc and Fab fragments from chicken immunoglobulin Y (IgY)

Journal of Chromatography B, 2020
Antigen-binding (Fab) and crystallizable (Fc) fragments are the active components of yolk immunoglobulin (IgY), which have been widely used in the pharmaceutical field. However, the common purification methods for the Fab and Fc fragments use combinations of multi-columns are complex and time-consuming.
Xin, Zhou   +3 more
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[Isolation of FAB and Fc fragments from murine immunoglobulin G1].

Biulleten' eksperimental'noi biologii i meditsiny, 1979
Two methods of isolating Fab- and Fc-fragments from mouse immunoglobulin G1 are presented. The first method involves fractionation of papain protein hydrolysate on a column with DEAE- (or DE-32)-cellulose adjusted with 0.005 M K-phosphate buffer, pH 8. The Fab-fragment was eluted from the column with the starting buffer.
E L, Beliaeva   +2 more
openaire   +1 more source

Distribution of Immunoglobulin Fab Fragment Conjugated with HIV-1 REV Peptide following Intravenous Administration in Rats

Molecular Pharmaceutics, 2005
HIV-1 REV peptide (positions 34-50) is well-known as a cell-permeating peptide. In this study, we investigated the distribution of Fab fragment of immunoglobulin conjugated with REV peptide (REV-Fab) following intravenous administration in rats, and compared with those of the native Fab fragment (nFab). Radioiodinated REV-Fab or nFab ((125)I-REV-Fab or
Shouju, Kameyama   +7 more
openaire   +2 more sources

Design and analysis of PCR primers for the amplification and cloning of human immunoglobulin Fab fragments

Human Antibodies, 1994
We have developed PCR primers for the amplification and cloning of the genes encoding human antibody fragments. Two sets of primers were designed to amplify the coding sequence of the complete variable region and the first constant domain of immunoglobulin heavy chains.
M, de Boer   +3 more
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Bacterial Proteins and Their Proposed Interactions with Fc or Fab Fragments of Immunoglobulins

Biochemistry & Physiology: Open Access, 2014
The reactivity of Immunoglobulin Binding Proteins (IBP) to Fc and/or Fab fragments of immunoglobulins was summarized in this review. Staphylococcal protein A (SpA), Streptococcal protein G and Peptostreptococcal protein L (SpL) were the IBP reported. SpA reacted with IgG from skunk, coyote, raccoon, mule and donkey.
openaire   +1 more source

Quantitative glycan profiling of normal human plasma derived immunoglobulin and its fragments Fab and Fc

Journal of Immunological Methods, 2012
Typical clinical grade human IgG (intravenous immunoglobulin, IVIG), used for carbohydrate analysis, is derived from thousands of healthy donors. Quantitative high-resolution glycan profiles of IgG and its Fc-Fab fragments are presented here. Glycan profiles were established following digestions with Fc specific endoglycosidase S and generic PNGase F ...
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immunoglobulines antidigoxine ovin (fragments Fab)

2009
Guillaume Chevrel, Virginie Dessus
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Isolation of Fab and Fc fragments from mouse immunoglobulin Gl

Bulletin of Experimental Biology and Medicine, 1979
E. L. Belyaeva   +2 more
openaire   +1 more source

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