Results 201 to 210 of about 101,155 (265)
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A comparison of strategies to stabilize immunoglobulin Fv-fragments
Biochemistry, 1990Fv-Fragments of antibodies may dissociate at low protein concentrations and are too unstable for many applications at physiological temperatures. To stabilize Fv-fragments against dissociation, we have tested and compared three different strategies on the Fv-fragment of the well-characterized phosphocholine binding antibody McPC603 expressed and ...
Andreas Plückthun
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Fragmentation of Immunoglobulin
AbstractThis unit describes procedures for fragmentation of IgG to the monovalent Fab fragment using papain digestion, and to the bivalent F(ab′)2 fragment by pepsin digestion. Alternative methods of fragmentation to F(ab′)2 include use of papain that is preactivated with cysteine and use of the enzyme ficin.
Sarah M, Andrew, Julie A, Titus
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FRAGMENTATION OF IMMUNOGLOBULIN DURING STORAGE
Canadian Journal of Biochemistry, 1966Solutions of human γ-globulin undergo changes on storage resulting in the formation of fragments similar to those produced by the digestion of human γ-globulin with the enzymes papain and plasmin. Two fragments have been isolated from the naturally fragmented material which resemble the Fab and Fc fragments resulting from digestion by papain.
G E, Connell, R H, Painter
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FRAGMENTS OF IMMUNOGLOBULINS IN HUMAN FAECES
Acta Pathologica Microbiologica Scandinavica Section C Immunology, 1976Fab‐fragments of IgG were easily demonstrated in extracts of faeces from healthy infants and children. Employing immunoelectrophoresis with antisera to whole human serum and to Fab‐fragments of IgG, a marked precipitation line, likely to represent such fragments, was evident in the cathodal or γ‐region.
B, Haneberg, C, Endresen
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Aggregation of immunoglobulin fragments in concentrated urine
Clinica Chimica Acta, 1967Abstract Normal human urine was concentrated by ultrafiltration and chromatographed on a Sephadex G-100 gel column. Fractions containing immunoglobulin subunits, either intact L and H polypeptide chains or fragments thereof were isolated, labelled with 131 I, diluted in the ultrafiltrate and subsequently reconcentrated by ultrafiltration. A portion
J R, Poortmans, K J, Bloch, R W, Jeanloz
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Coprecipitation of some cryomacroglobulins with immunoglobulins and their fragments
Archives of Biochemistry and Biophysics, 1965Abstract Four cryomacroglobulins, isolated from different pathological sera, were found to coprecipitate on cooling with normal IGG. Coprecipitation occurred also with the immunoelectrophoretically fast component, and to a much lesser extent with the slow component, of papain digests of IGG and the cryomacroglobulins.
C C, Curtain, A, Baumgarten, J, Pye
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Immunoglobulin genes in DNA restriction fragments
Biochemistry, 1978We have investigated the organization of immunoglobulin genes in mice. High molecular weight DNA from myelomas and Krebs ascites cells was cleaved with EcoRI restriction endonuclease and fractionated using preparative agarose gel electrophoresis. Each fraction was then hybridized to an immunoglobulin mRNA or a cDNA transcribed from the mRNA.
R, Wilson +3 more
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Fragmentation of Therapeutic Human Immunoglobulin Preparations
Vox Sanguinis, 1995AbstractSome commercial batches of human therapeutic immunoglobulins (Ig) have been found to show evidence of molecular fragmentation when examined by molecular sizing methodologies including sodium dodecyl sulphate polyacrylamide gel electrophoresis [SDS‐PAGE] and size exclusion high performance liquid chromatography (SE‐HPLC).
M, Page +6 more
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Aggregation of an immunoglobulin fragment by sulfhydryl oxidation
Canadian Journal of Biochemistry, 1968An immunoglobulin of the IgG family has been isolated from the serum of a patient with multiple myeloma. The IgG was subjected to plasmin digestion and the resulting Fab fragment was isolated and characterized. Incubation of this fragment in denaturing solvents, such as aqueous 8 M urea at either pH 3.4 or pH 8.2, resulted in the formation of a dimer ...
C I, Luks, G E, Connell
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