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Immunoglobulin isotypes in childhood asthma
The Indian Journal of Pediatrics, 1999Immunoglobulin isotypes (IgG, IgA, IgM, IgD, IgE) in serum were investigated in 64 Libyan children with mild to moderately severe asthma (age: 1-12 years; sex: 39 males, 25 females) (Group A) and in 57 healthy Libyan children (age: 1-12 years; sex: 30 males, 27 females (Group B).
F I, Najam +2 more
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Immunoglobulin Isotypes: Structure, Function, and Genetics
Current Topics in Microbiology and Immunology, 2000Immunoglobulin (Ig) classes (in mammals, IgM, IgA, IgD, IgG, IgE) are defined by the isotypes of heavy (H) chains (µ, α, δ, γ, and e). Each isotype is in turn distinguished by unique structures in its constant region domains. These different structures confer distinctive functions on the Ig classes.
E, Bengtén +5 more
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Reduction chemistry-assisted nanopore determination method for immunoglobulin isotypes.
Nanoscale, 2020Immunoglobulins can bind to an unlimited array of foreign antigens presented to the immune system. Among those isotypes, IgG and IgM play crucial roles in initial immune defense associated with innate immunity factors. Hence, the determination of IgG and
Qianshan Liu +11 more
semanticscholar +1 more source
Veterinary Immunology and Immunopathology, 1997
Turkey immunoglobulin (Ig) isotypes IgG and IgM were isolated from blood and IgA was isolated from bile. Isolation was accomplished by gel filtration of the ammonium sulphate cut on Sephacryl S-200. Using immunoelectrophoresis and indirect ELISA, the cross-reactivity between antibodies, of monoclonal and polyclonal origin, specific for the Ig isotypes ...
A, Van Nerom +6 more
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Turkey immunoglobulin (Ig) isotypes IgG and IgM were isolated from blood and IgA was isolated from bile. Isolation was accomplished by gel filtration of the ammonium sulphate cut on Sephacryl S-200. Using immunoelectrophoresis and indirect ELISA, the cross-reactivity between antibodies, of monoclonal and polyclonal origin, specific for the Ig isotypes ...
A, Van Nerom +6 more
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Monoclonal Antibodies to Murine Immunoglobulin Isotypes
Hybridoma, 1983We have obtained cell lines producing monoclonal antibodies against mu, alpha, lambda, and lambda 1 by fusing the mouse myeloma P3X63Ag8.653 with spleen cells from Lewis rats immunized with several lambda 1 bearing myeloma proteins. The anti-lambda 1 antibody is particularly useful.
S, Weiss, K, Lehmann, M, Cohn
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Immunoglobulin Isotype Expression
1978Many different kinds of observations suggest that during development of the B cell line, individual V H genes determining the antibody specificity of an immunoglobulin molecule can be expressed with multiple C H genes. Thus each clone of B cells, defined by expression of a variable-region gene for the light and heavy chains of the immunoglobulin ...
J F, Kearney, E R, Abney
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Monoclonal antibodies against porcine immunoglobulin isotypes
Veterinary Immunology and Immunopathology, 1987Monoclonal antibodies (MCAs) against porcine immunoglobulin isotypes* G, G1, G2, M and A have been produced and characterized in detail. Epitope analysis using a competitive direct enzyme-linked immunosorbent assay (ELISA) indicated that the MCAs recognized 3 class-specific epitopes of IgG, 4 epitopes specific for IgG1, 3 epitopes specific for IgG2, 2 ...
D, Van Zaane, M M, Hulst
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Isoelectric restriction of human immunoglobulin isotypes
Biochimica et Biophysica Acta (BBA) - General Subjects, 1995The partition of human serum immunoglobulins along a pH gradient of ampholynes was investigated using the recently developed method of preparative isoelectrofocusing. Each isotype was demonstrated to display a specific pI range, with limited overlapping. IgA appear to be the most acidic serum immunoglobulins while IgG are clearly basic.
C, Prin +4 more
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Immunoglobulin isotype switching in xid mice
Molecular Immunology, 1995Mice with the x-linked immunodeficiency mutation (xid) are unresponsive to polysaccharide antigens, lack a subset of B cells, and have low serum IgM (2-20% of normal) and IgG3 (3% of normal). Because of the disproportionate reduction of IgG3, the ability of B cells from xid mice to switch to gamma 3 was examined.
K A, Brorson +2 more
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Immunoglobulin Isotypes in Human Minor Gland Saliva
Journal of Dental Research, 1991Lower labial, upper labial, palatine (minor), and parotid (major) gland saliva samples from 18 young adult males were quantitatively assayed for the presence of IgAl, IgA2, IgM, and IgG. The mean (± standard deviation) concentrations of IgA (sum of IgAl + IgA2) were 79 ± 42 μg/mL (parotid), 111 ± 42 μg/mL (lower labial), 69 ± 72 μg/mL (upper labial ...
D J, Smith, M A, Taubman, P, Ali-Salaam
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