Results 11 to 20 of about 278,035 (347)

Fluorescence quenching by high-power LEDs for highly sensitive fluorescence in situ hybridization

Frontiers in Molecular Neuroscience, 2022
Recent technical advances have made fluorescent in situ hybridization (ISH) a pivotal method to analyze neural tissue. In a highly sensitive ISH, it is important to reduce tissue autofluorescence.
Yousuke Tsuneoka, Yusuke Atsumi, Yusuke Atsumi, Aki Makanae, Aki Makanae, Mitsuru Yashiro, Mitsuru Yashiro, Hiromasa Funato, Hiromasa Funato   +8 more
doaj   +2 more sources

Advances and Challenges in Fluorescence in situ Hybridization for Visualizing Fungal Endobacteria

Frontiers in Microbiology, 2022
Several bacteria have long been known to interact intimately with fungi, but molecular approaches have only recently uncovered how cosmopolitan these interactions are in nature.
Demosthenes P. Morales, Aaron J. Robinson, Andrew C. Pawlowski, Andrew C. Pawlowski, Caitlyn Ark, Julia M. Kelliher, Pilar Junier, James H. Werner, Patrick S. G. Chain   +8 more
doaj   +2 more sources

Imaging biofilms using fluorescence in situ hybridization: seeing is believing

Frontiers in Cellular and Infection Microbiology, 2023
Biofilms are complex structures with an intricate relationship between the resident microorganisms, the extracellular matrix, and the surrounding environment.
Ana Barbosa, Sónia Miranda, N. Azevedo, L. Cerqueira, A. Azevedo   +4 more
semanticscholar   +1 more source

Microfluidics combined with fluorescence in situ hybridization (FISH) for Candida spp. detection

Frontiers in Bioengineering and Biotechnology, 2022
One of the most prevalent healthcare-associated infection is the urinary tract infection (UTI), caused by opportunistic pathogens such as Candida albicans or non-albicans Candida species (NACS).
V. Barbosa, C. F. Rodrigues, L. Cerqueira, J. Miranda, N. Azevedo   +4 more
semanticscholar   +1 more source

An efficient fluorescence in situ hybridization (FISH)-based circulating genetically abnormal cells (CACs) identification method based on Multi-scale MobileNet-YOLO-V4.

Quantitative Imaging in Medicine and Surgery, 2022
Background Circulating tumor cells (CTCs) acting as "liquid biopsy" of cancer are cells that have been shed from the primary tumor, which cause the development of a secondary tumor in a distant organ site, leading to cancer metastasis.
Chao Xu, Yi Zhang, Xianjun Fan, X. Lan, X. Ye, Tongning Wu   +5 more
semanticscholar   +1 more source

Spectral imaging and nucleic acid mimics fluorescence in situ hybridization (SI-NAM-FISH) for multiplex detection of clinical pathogens

Frontiers in Microbiology, 2022
The application of nucleic acid mimics (NAMs), such as locked nucleic acid (LNA) and 2′-O-methyl-RNA (2’OMe), has improved the performance of fluorescence in situ hybridization (FISH) methods for the detection/location of clinical pathogens since they ...
A. Azevedo, R. Fernandes, A. R. Faria, O. Silvestre, J. Nieder, Chenguang Lou, J. Wengel, Carina Almeida, N. Azevedo   +8 more
semanticscholar   +1 more source

Karyotype Differentiation in Cultivated Chickpea Revealed by Oligopainting Fluorescence in situ Hybridization

Frontiers in Plant Science, 2022
Chickpea (Cicer arietinum L.) is one of the main sources of plant proteins in the Indian subcontinent and West Asia, where two different morphotypes, desi and kabuli, are grown.
Alžběta Doležalová, Lucia Sládeková, D. Šimoníková, K. Holušová, M. Karafiátová, R. Varshney, J. Doležel, E. Hřibová   +7 more
semanticscholar   +1 more source

Detection of HPV and Human Chromosome Sites by Dual-Color Fluorescence In Situ Hybridization Reveals Recurrent HPV Integration Sites and Heterogeneity in Cervical Cancer

Frontiers in Oncology, 2021
Human papillomavirus (HPV) integration in the human genome is suggested to be an important cause of cervical cancer. With the development of sequencing technologies, an increasing number of integration “hotspots” have been identified.
Jinfeng Xiong, Jing Cheng, Hui Shen, Ci Ren, Liming Wang, Chun Gao, T. Zhu, Xiaomin Li, W. Ding, Da Zhu, Hui Wang   +10 more
semanticscholar   +1 more source

Bovine Abortions Revisited—Enhancing Abortion Diagnostics by 16S rDNA Amplicon Sequencing and Fluorescence in situ Hybridization

Frontiers in Veterinary Science, 2021
Abortion in cattle causes significant economic losses for cattle farmers worldwide. The diversity of abortifacients makes abortion diagnostics a complex and challenging discipline that additionally is restrained by time and economy. Microbial culture has
G. Wolf-Jäckel, M. Strube, Kirstine Klitgaard Schou, C. Schnee, J. Agerholm, T. Jensen   +5 more
semanticscholar   +1 more source

Double-staining chromogenic in situ hybridization as a useful alternative to split-signal fluorescence in situ hybridization in lymphoma diagnostics

Haematologica, 2010
Background Malignant lymphomas are classified based on morphology, immunophenotype, genetics and clinical features. The pathological diagnosis is generally considered difficult and prone to mistakes.
Anke van Rijk, Tim Svenstroup-Poulsen, Margaret Jones, José Cabeçadas, Juan Cruz Cigudosa, Lorenzo Leoncini, Anja Mottok, Christiane Copie Bergman, Evi Pouliou, Stephen Hamilton Dutoit, Han J. van Krieken   +10 more
doaj   +1 more source