Mid-Infrared Photothermal-Fluorescence in Situ Hybridization for Functional Analysis and Genetic Identification of Single Cells [PDF]
arXiv, 2022 Simultaneous identification and metabolic analysis of microbes with single-cell resolution and high throughput is necessary to answer the question of "who eats what, when, and where" in complex microbial communities. Here, we present a mid-infrared photothermal-fluorescence in situ hybridization (MIP-FISH) platform that enables direct bridging of ...
arxiv
In Situ Hybridization Pada Kanker Payudara [PDF]
, 2014 Kesulitan yang dijumpai pada penanganan kanker payudara adalah terjadinya kekambuhan atau relaps. Deteksi status HER2 pada pasien merupakan salah satu upaya untuk mendeteksi terjadinya relaps dan juga untuk menentukan jenis terapi yang ada diberikan ...
Diah Witari, Ni Putu
core +2 more sources
Size and location of radish 1 chromosome regions carrying the fertility restorer Rfk1 gene in spring turnip rape [PDF]
Chromosome Research 2012, 20(3): 353-361, 2012 In spring turnip rape (Brassica rapa L. spp. oleifera) the most promising F1 hybrid system would be the Ogu-INRA CMS/Rf system. A Kosena fertility restorer gene Rfk1, homologue of the Ogura restorer gene Rfo, was successfully transferred from oilseed rape into turnip rape and that restored the fertility in female lines carrying Ogura cms. The trait was,
arxiv +1 more source
Detection and enumeration of periodontopathogenic bacteria in subgingival biofilm of pregnant women
Brazilian Oral Research, 2012 The aim of this study was to use the fluorescence in situ hybridization (FISH) technique to test the hypothesis of qualitative and quantitative differences of 8 periodontopathogens between pregnant and non-pregnant women.
Fernanda Campos Machado +4 more
doaj +1 more source
Development of a Biological Dosimeter for Translocation Scoring Based on Two-Color Fluorescence in Situ Hybridization of Chromosome Subsets [PDF]
, 1992 Recently fluorescence in situ hybridization protocols have been developed which allow the paining of individual chromosomes using DNA-libraries from sorted human chromosomes.
Cremer, Thomas, Popp, Susanne
core +1 more source
A simplified and rapid in situ hybridization protocol for planarians
BioTechniques, 2023 Whole-mount in situ hybridization is a critical technique for analyzing gene expression in planarians. While robust in situ protocols have been developed, these protocols are laborious, making them challenging to incorporate in an academic setting ...
Andrew J Gaetano, Ryan S King
doaj +1 more source
Rapid metaphase and interphase detection of radiation-induced chromosome aberrations in human lymphocytes by chromosomal suppression in situ hybridization [PDF]
, 1990 Chromosomal in situ suppression (CISS)-hybridization of biotinylated phage DNA-library inserts from sorted human chromosomes was used to decorate chromosomes 1 and 7 specifically from pter to qter and to detect structural aberrations of these chromosomes
Cremer, Christoph +4 more
core +1 more source
A strategy for the characterization of minute chromosome rearrangements using multiple color fluorescence in situ hybridization with chromosome-specific DNA libraries and YAC clones [PDF]
, 1993 The identification of marker chromosomes in clinical and tumor cytogenetics by chromosome banding analysis can create problems. In this study, we present a strategy to define minute chromosomal rearrangements by multicolor fluorescence in situ ...
A Kallioniemi +31 more
core +1 more source
Controlled plasmon-enhanced fluorescence by spherical microcavity [PDF]
, 2021 A surrounding electromagnetic environment can engineer spontaneous emissions from quantum emitters through the Purcell effect. For instance, a plasmonic antenna can efficiently confine an electromagnetic field and enhance the fluorescent process. In this study, we demonstrate that a photonic microcavity can modulate plasmon-enhanced fluorescence by ...
arxiv +1 more source
Detection of chromosome aberrations in the human interphase nucleus by visualization of specific target DNAs with radioactive and non-radioactive in situ hybridization techniques: diagnosis of trisomy 18 with probe L1.84 [PDF]
, 1986 The localization of chromosome 18 in human interphase nuclei is demonstrated by use of radioactive and nonradioactive in situ hybridization techniques with a DNA clone designated L1.84.
A. Brückner +33 more
core +1 more source