Results 251 to 260 of about 459,673 (306)
Some of the next articles are maybe not open access.
2003
In situhybridization permits specific identification of genes and gene expression without removing the target sequence from its topographical surroundings. The technique was described simultaneously in 1960 by two groups (1,2). It depends on the hybridization of a labeled nucleic acid probe to a complementary sequence of tissue mRNA or DNA.
C G, Beckett, P J, Ciclitira
+13 more sources
In situhybridization permits specific identification of genes and gene expression without removing the target sequence from its topographical surroundings. The technique was described simultaneously in 1960 by two groups (1,2). It depends on the hybridization of a labeled nucleic acid probe to a complementary sequence of tissue mRNA or DNA.
C G, Beckett, P J, Ciclitira
+13 more sources
Ultrastructural Pathology, 1989
Twenty years ago Gall and Pardue1 reported a method to detect RNA-DNA hybrid molecules in cytologic specimens. Since then diagnostic in situ hybridization has become a standard method to detect DNA and RNA.The method has also been modified for use in electron microscopy2-4 but it is not easy to preserve both the hybridization properties and an ...
J M, Nesland, J V, Johannessen
openaire +3 more sources
Twenty years ago Gall and Pardue1 reported a method to detect RNA-DNA hybrid molecules in cytologic specimens. Since then diagnostic in situ hybridization has become a standard method to detect DNA and RNA.The method has also been modified for use in electron microscopy2-4 but it is not easy to preserve both the hybridization properties and an ...
J M, Nesland, J V, Johannessen
openaire +3 more sources
2013
A non-radioactive in situ hybridization protocol is presented for localization of mRNA transcripts in thin tissue sections. In situ hybridization provides spatial resolution of transcript distribution at the cellular level that is not attained by Northern hybridization or PCR-based methods using organ- or tissue-derived RNA. With appropriately designed
Chung-Jui, Tsai, Scott A, Harding
openaire +2 more sources
A non-radioactive in situ hybridization protocol is presented for localization of mRNA transcripts in thin tissue sections. In situ hybridization provides spatial resolution of transcript distribution at the cellular level that is not attained by Northern hybridization or PCR-based methods using organ- or tissue-derived RNA. With appropriately designed
Chung-Jui, Tsai, Scott A, Harding
openaire +2 more sources
Clinics in Dermatology, 1991
Abstract Although several molecular biology techniques can be used to measure mRNA, only in situ hybridization (or in situ transcription) permit specific localization of DNA or an mRNA species within a tissue section or cell preparation. With appropriate fixation, mRNAs can be preserved and detected in tissue sections by using DNA or RNA probes ...
+6 more sources
Abstract Although several molecular biology techniques can be used to measure mRNA, only in situ hybridization (or in situ transcription) permit specific localization of DNA or an mRNA species within a tissue section or cell preparation. With appropriate fixation, mRNAs can be preserved and detected in tissue sections by using DNA or RNA probes ...
+6 more sources
Clinics in Laboratory Medicine, 1990
In situ hybridization is a technique with wide application in laboratory medicine. In this article, the basic and technical aspects of in situ hybridization are reviewed, and applications are discussed that emphasize methods for the detection of gene expression.
openaire +2 more sources
In situ hybridization is a technique with wide application in laboratory medicine. In this article, the basic and technical aspects of in situ hybridization are reviewed, and applications are discussed that emphasize methods for the detection of gene expression.
openaire +2 more sources
Metallographic in situ hybridization
Human Pathology, 2007Metallographic methods, in which a target is visualized using a probe or antibody that deposits metal selectively at its binding site, offers many advantages for bright-field in situ hybridization (ISH) detection as well as for other labeling and detection methods.
Richard D, Powell +6 more
openaire +2 more sources
Measurement of In Situ Hybridization
Current Protocols in Molecular Biology, 2000AbstractHybridization of labeled specific molecular probes to nucleic acids in tissues allows geometric and functional location of gene expression or of foreign genome sequences. Estimates of amounts and location of target nucleic acid sequence can be made with phosphor storage imaging and molecular controls.
S B, Hoover +4 more
openaire +2 more sources
Fluorescence In Situ Hybridization
2003In situ hybridization describes the annealing of a labeled nucleic acid to complementary nucleic acid sequences in a fixed target (e.g., chromosomes, free nuclei, nuclei in tissue sections, and DNA) followed by visualisation of the location of the probe.
Barbara A, Tate, Rachel L, Ostroff
openaire +4 more sources
2008
Hybridization is the formation of hybrid nucleic acid molecules with complementary nucleotide sequences in DNA:DNA, DNA:RNA, or RNA:RNA forms. In situ hybridization is a highly sensitive technique that allows detection and localization of specific DNA or RNA molecules in morphologically preserved isolated cells, histological tissue sections, or ...
openaire +2 more sources
Hybridization is the formation of hybrid nucleic acid molecules with complementary nucleotide sequences in DNA:DNA, DNA:RNA, or RNA:RNA forms. In situ hybridization is a highly sensitive technique that allows detection and localization of specific DNA or RNA molecules in morphologically preserved isolated cells, histological tissue sections, or ...
openaire +2 more sources