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Molecular Fingerprint of Endocannabinoid Signaling in the Developing Paraventricular Nucleus of the Hypothalamus as Revealed by Single-Cell RNA-Seq and In Situ Hybridization. [PDF]
CellsTretiakov EO +5 more
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Precise identification of intersectional hybrids in <i>Morus</i> using genomic <i>in situ</i> hybridization (GISH). [PDF]
For Res (Fayettev)Zhou Q +7 more
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Fluorescent In Situ Hybridization Testing Allows the Diagnosis of <i>NRG1</i> Gene Fusions in Lung and Pancreas Cancers with No Other Identified Oncogenic Driver. [PDF]
Cancers (Basel)Bastard C +9 more
europepmc +1 more source
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2003
In situhybridization permits specific identification of genes and gene expression without removing the target sequence from its topographical surroundings. The technique was described simultaneously in 1960 by two groups (1,2). It depends on the hybridization of a labeled nucleic acid probe to a complementary sequence of tissue mRNA or DNA.
C G, Beckett, P J, Ciclitira
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In situhybridization permits specific identification of genes and gene expression without removing the target sequence from its topographical surroundings. The technique was described simultaneously in 1960 by two groups (1,2). It depends on the hybridization of a labeled nucleic acid probe to a complementary sequence of tissue mRNA or DNA.
C G, Beckett, P J, Ciclitira
+12 more sources
Ultrastructural Pathology, 1989
Twenty years ago Gall and Pardue1 reported a method to detect RNA-DNA hybrid molecules in cytologic specimens. Since then diagnostic in situ hybridization has become a standard method to detect DNA and RNA.The method has also been modified for use in electron microscopy2-4 but it is not easy to preserve both the hybridization properties and an ...
J M, Nesland, J V, Johannessen
openaire +3 more sources
Twenty years ago Gall and Pardue1 reported a method to detect RNA-DNA hybrid molecules in cytologic specimens. Since then diagnostic in situ hybridization has become a standard method to detect DNA and RNA.The method has also been modified for use in electron microscopy2-4 but it is not easy to preserve both the hybridization properties and an ...
J M, Nesland, J V, Johannessen
openaire +3 more sources
Clinics in Laboratory Medicine, 1990
In situ hybridization is a technique with wide application in laboratory medicine. In this article, the basic and technical aspects of in situ hybridization are reviewed, and applications are discussed that emphasize methods for the detection of gene expression.
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In situ hybridization is a technique with wide application in laboratory medicine. In this article, the basic and technical aspects of in situ hybridization are reviewed, and applications are discussed that emphasize methods for the detection of gene expression.
openaire +2 more sources
2013
A non-radioactive in situ hybridization protocol is presented for localization of mRNA transcripts in thin tissue sections. In situ hybridization provides spatial resolution of transcript distribution at the cellular level that is not attained by Northern hybridization or PCR-based methods using organ- or tissue-derived RNA. With appropriately designed
Chung-Jui, Tsai, Scott A, Harding
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A non-radioactive in situ hybridization protocol is presented for localization of mRNA transcripts in thin tissue sections. In situ hybridization provides spatial resolution of transcript distribution at the cellular level that is not attained by Northern hybridization or PCR-based methods using organ- or tissue-derived RNA. With appropriately designed
Chung-Jui, Tsai, Scott A, Harding
openaire +2 more sources
Clinics in Dermatology, 1991
Abstract Although several molecular biology techniques can be used to measure mRNA, only in situ hybridization (or in situ transcription) permit specific localization of DNA or an mRNA species within a tissue section or cell preparation. With appropriate fixation, mRNAs can be preserved and detected in tissue sections by using DNA or RNA probes ...
+6 more sources
Abstract Although several molecular biology techniques can be used to measure mRNA, only in situ hybridization (or in situ transcription) permit specific localization of DNA or an mRNA species within a tissue section or cell preparation. With appropriate fixation, mRNAs can be preserved and detected in tissue sections by using DNA or RNA probes ...
+6 more sources