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Applications of in Situ Hybridization
1991Publisher Summary This chapter discusses applications of in situ hybridization(ISH). Progress in molecular biology has made several techniques for research and diagnosis available even to laboratories not specialized in this field. The basis of all these techniques is the ability of single-stranded nucleic acids, either deoxyribonucleic acid or ...
G, Niedobitek, H, Herbst
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MicroRNA In Situ Hybridization
2011In situ hybridization (ISH) is a technology that allows detection of specific nucleic acid sequences in tissue samples at the cellular level. For detection of individual microRNAs (miRNAs) and mRNAs, the ISH technology determines the cellular origin of expression and provides information on expression levels in different tissue compartments and cell ...
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In situ hybridization of nucleic acids
Trends in Biochemical Sciences, 1976Hybridization of radioactive nucleic acid sequences can be carried out with DNA in cytological preparations. By autoradiography distinct DNA sequences can then be localized in eukaryotic chromosomes.
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Strategies forin situ hybridization
La Ricerca in Clinica e in Laboratorio, 1988To the casual observer, in situ hybridization (ISH) suggests a method to detect and localize nucleic acid sequences in cell preparations, tissue sections or chromosome spreads. This paper is an attempt to demonstrate that ISH is very much more versatile than is immediately apparent; a number of possible alternatives in different steps in the procedure ...
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2009
Nucleic acid hybridization techniques have contributed significantly to the understanding of gene organization, regulation and expression. In situ hybridization is a method for detecting specific nucleotide sequences by using a labeled complementary nucleic acid probe.
Mousumi Debnath +2 more
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Nucleic acid hybridization techniques have contributed significantly to the understanding of gene organization, regulation and expression. In situ hybridization is a method for detecting specific nucleotide sequences by using a labeled complementary nucleic acid probe.
Mousumi Debnath +2 more
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1993
Abstract The visualization of patterns of gene expression in embryos is an essential technique for the molecular analysis of development. The detection of mRNA by in situ hybridization has found widespread use because of the speed with which specific probes can be produced from cloned DNA or by the synthesis of oligonucleotides.
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Abstract The visualization of patterns of gene expression in embryos is an essential technique for the molecular analysis of development. The detection of mRNA by in situ hybridization has found widespread use because of the speed with which specific probes can be produced from cloned DNA or by the synthesis of oligonucleotides.
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Single-cell in situ RNA profiling by sequential hybridization
Nature Methods, 2014Eric Lübeck +2 more
exaly