Results 261 to 270 of about 459,673 (306)

Fluorescence In Situ Hybridization

Clinics in Laboratory Medicine, 2011
This chapter presents past and present FISH techniques and specific applications of FISH. Although array technology has revolutionized cytogenetics, FISH remains indispensible. While array technology provides a high resolution screen of the entire genome for gains and losses, it does not allow for visualization of the genomic structure of gains.
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Fluorescence in situ hybridization

Blood Reviews, 1993
Spectacular advances in the use of fluorescence in situ hybridization (FISH) for the visualisation of specific DNA sequences in metaphase chromosomes and interphase cells have been made over the last few years making the technique a useful tool in clinical research.
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Fluorescent In Situ Hybridization

2003
Single-stranded DNA will recognize a complementary strand with high specificity under suitably controlled conditions. In situ hybridization (ISH) exploits this phenomenon by hybridizing an appropriately labeled singlestranded DNA “probe” to target sequences in situ in either dissociated cell preparations or tissue sections.
Sara A, Dyer, Elaine K, Green
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The Technique of In Situ Hybridization

2003
In situ hybridization (ISH) (also called "hybridization histochemistry" or "hybridization cytology") was first described in 1969 by Gall and Pardue who used the technique to localize ribosomal DNA in Xenopus oocytes (1). In contrast to other techniques of hybridization (i.e., Northern or Southern blotting), ISH allows detection of specific DNA or RNA ...
S, Ying, A, Barry Kay
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Ultrastructure of in Situ Hybridization

Ultrastructural Pathology, 1992
Techniques for the ultrastructural localization of structures identified by in situ hybridization are being developed for both preembedding labeling and labeling on thin sections (postembedding). Successful labeling of both RNA and DNA sequences has been reported in recent years.
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In Situ Hybridization Histochemistry

Current Protocols in Toxicology, 2000
AbstractThis unit describes two methods of in situ hybridization: one uses an 35S‐labeled oligonucleotide probe and the other uses a digoxigenin‐labeled oligonucleotide probe on frozen, cryostat‐sectioned samples. These methods allow detection of the physical distribution and expression levels of target mRNA.
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Applications of in Situ Hybridization

1991
Publisher Summary This chapter discusses applications of in situ hybridization(ISH). Progress in molecular biology has made several techniques for research and diagnosis available even to laboratories not specialized in this field. The basis of all these techniques is the ability of single-stranded nucleic acids, either deoxyribonucleic acid or ...
G, Niedobitek, H, Herbst
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MicroRNA In Situ Hybridization

2011
In situ hybridization (ISH) is a technology that allows detection of specific nucleic acid sequences in tissue samples at the cellular level. For detection of individual microRNAs (miRNAs) and mRNAs, the ISH technology determines the cellular origin of expression and provides information on expression levels in different tissue compartments and cell ...
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Strategies forin situ hybridization

La Ricerca in Clinica e in Laboratorio, 1988
To the casual observer, in situ hybridization (ISH) suggests a method to detect and localize nucleic acid sequences in cell preparations, tissue sections or chromosome spreads. This paper is an attempt to demonstrate that ISH is very much more versatile than is immediately apparent; a number of possible alternatives in different steps in the procedure ...
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In Situ Hybridization

2009
Nucleic acid hybridization techniques have contributed significantly to the understanding of gene organization, regulation and expression. In situ hybridization is a method for detecting specific nucleotide sequences by using a labeled complementary nucleic acid probe.
Mousumi Debnath, Godavarthi B.K.S. Prasad, Prakash S. Bisen   +2 more
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