Introduction : HER2/neu gene status in breast cancers can be evaluated by targeting protein and gene - immunohistochemistry (IHC) and fluorescence in-situ hybridization (FISH).
Hemlata S Shirsat +5 more
doaj +1 more source
Mucormycosis is an invasive fungal infection associated with high mortality, partly due to delayed diagnosis and inadequate empiric therapy. As fungal cultures often fail to grow Mucorales, identification of respective hyphae in tissue is frequently ...
Jill Jasmine Dalimot +7 more
doaj +1 more source
In situ detection of mature microRNAs by labeled extension on ultramer templates
We describe a new method for the in situ detection of a mature microRNA (miRNA) in formalin-fixed, paraffin-embedded tissues. The method involves the labeled extension of miRNA hybridized to an approximately 100-nucleotide–long ultramer template ...
Gerard J. Nuovo +4 more
doaj +1 more source
Multimodal mapping of cell types and projections in the central nucleus of the amygdala
The central nucleus of the amygdala (CEA) is a brain region that integrates external and internal sensory information and executes innate and adaptive behaviors through distinct output pathways. Despite its complex functions, the diversity of molecularly
Yuhan Wang +10 more
doaj +1 more source
Detection of fluorescence in situ hybridization on human metaphase chromosomes by near-field scanning optical microscopy [PDF]
Fluorescence in situ hybridization signals on human metaphase chromosomes are detected by a near-field scanning optical microscope. This makes it possible to localize and identify several fluorescently labeled genomic DNA fragments on a single ...
Jalocha, A. +5 more
core +3 more sources
Escherichia coli‐associated follicular cystitis in dogs: Clinical and pathologic characterization
Background Follicular cystitis is an uncommon inflammatory change in the urinary bladder wall characterized by the formation of tertiary lymphoid structures (TLSs) in the submucosa.
Sanna J. Viitanen +4 more
doaj +1 more source
Rapid generation of chromosome-specific alphoid DNA probes using the polymerase chain reaction [PDF]
Non-isotopic in situ hybridization of chromosome-specific alphoid DNA probes has become a potent tool in the study of numerical aberrations of specific human chromosomes at all stages of the cell cycle. In this paper, we describe approaches for the rapid
A Gnirke +33 more
core +1 more source
Translocation (8;21) in acute nonlymphocytic leukemia delineated by chromosomal in situ suppression hybridization [PDF]
In situ suppression hybridization with recombinant bacteriophage DNA libraries for chromosomes 8 and 21 was performed in two cases of acute nonlymphocytic leukemia, type FAB M2.
Anna Jauch +23 more
core +1 more source
Visualizing the Needle in the Haystack: In Situ Hybridization With Fluorescent Dendrimers
In situ hybridization with 3DNA™ dendrimers is a novel tool for detecting low levels of mRNA in tissue sections and whole embryos. Fluorescently labeled dendrimers were used to identify cells that express mRNA for the skeletal muscle transcription ...
Gerhart Jacquelyn +8 more
doaj +1 more source
Significance of molecular diagnostics in human papilloma virus (HPV) determination [PDF]
HPV infection is considered to be the most important etiologic factor in cervical cancer development. In this retrospective study, which included the period from 2000 to 2012, the results of two molecular techniques used in the detection of HPV ...
Kovačević G. +8 more
doaj +1 more source

