Results 21 to 30 of about 370,888 (338)

Analysis of Structural Genomic Diversity in Aegilops umbellulata, Ae. markgrafii, Ae. comosa, and Ae. uniaristata by Fluorescence In Situ Hybridization Karyotyping

Frontiers in Plant Science, 2020
Fluorescence in situ hybridization karyotypes have been widely used for evolutionary analysis on chromosome organization and genetic/genomic diversity in the wheat alliance (tribe Triticeae of Poaceae). The karyotpic diversity of Aegilops umbellulata, Ae.
Zhongping Song, S. Dai, Tingyu Bao, Yuanyuan Zuo, Qin Xiang, Jian Li, Gang Liu, Zehong Yan   +7 more
semanticscholar   +1 more source

In situ hybridization in paracoccidioidomycosis [PDF]

Medical Mycology, 1999
In situ hybridization (ISH) was performed using oral biopsies from patients with paracoccidioidomycosis and guinea pig testes inoculated with a culture of Paracoccidioides brasiliensis isolated from soil, employing both a 14 base-pair specific oligoprobe (ACT CCC CCG TGG TC) and its complementary sequence.
A. A. H. Brandão, Bruce C. Kline, Gurpreet S. Sandhu, R. T. M. Santos, T. De Brito, R A Aleff, Carlos da Silva Lacaz, M P Sandoval   +7 more
openaire   +3 more sources

HER 2 status in invasive breast cancer: Immunohistochemistry, fluorescence in-situ hybridization and chromogenic in-situ hybridization

Indian Journal of Pathology and Microbiology, 2012
Introduction : HER2/neu gene status in breast cancers can be evaluated by targeting protein and gene - immunohistochemistry (IHC) and fluorescence in-situ hybridization (FISH).
Hemlata S Shirsat, Sridhar Epari, Tanuja Shet, Rajani Bagal, Rohini Hawaldar, Sangeeta B Desai   +5 more
doaj   +1 more source

In situ detection of mature microRNAs by labeled extension on ultramer templates

BioTechniques, 2009
We describe a new method for the in situ detection of a mature microRNA (miRNA) in formalin-fixed, paraffin-embedded tissues. The method involves the labeled extension of miRNA hybridized to an approximately 100-nucleotide–long ultramer template ...
Gerard J. Nuovo, Eun Joo Lee, Sean Lawler, Jakub Godlewski, Thomas D. Schmittgen   +4 more
doaj   +1 more source

Significance of molecular diagnostics in human papilloma virus (HPV) determination [PDF]

Archives of Biological Sciences, 2014
HPV infection is considered to be the most important etiologic factor in cervical cancer development. In this retrospective study, which included the period from 2000 to 2012, the results of two molecular techniques used in the detection of HPV ...
Kovačević G., Milošević V., Hrnjaković-Cvjetković I., Petrović V., Stefan-Mikić S., Radovanov J., Jovanović-Galović A., Patić A., Elez I.   +8 more
doaj   +1 more source

Step-by-step preparation of mouse eye sections for routine histology, immunofluorescence, and RNA in situ hybridization multiplexing

STAR Protocols, 2021
Summary: It can be challenging to maintain tissue integrity using established histology protocols. Here, we describe a protocol composed of Hartman’s fixation, window technique, microwave-based tissue processing, optimized depigmentation, and antigen ...
Ji Pang, Nancy Thomas, Dai Tsuchiya, Tari Parmely, Deyue Yan, Ting Xie, Yongfu Wang   +6 more
doaj  

Visualizing the Needle in the Haystack: In Situ Hybridization With Fluorescent Dendrimers

Biological Procedures Online, 2004
In situ hybridization with 3DNA™ dendrimers is a novel tool for detecting low levels of mRNA in tissue sections and whole embryos. Fluorescently labeled dendrimers were used to identify cells that express mRNA for the skeletal muscle transcription ...
Gerhart Jacquelyn, Baytion Michael, Perlman Jordanna, Neely Christine, Hearon Bridget, Nilsen Thor, Getts Robert, Kadushin James, George-Weinstein Mindy   +8 more
doaj   +1 more source

Revealing details: whole mount microRNA in situ hybridization protocol for zebrafish embryos and adult tissues

Biology Open, 2012
Summary Non-coding microRNA (miRNA) molecules bind their target mRNAs and thereby modulate the amount of protein produced. To understand the significance of a potential miRNA-mRNA interaction, temporal and spatial information on miRNA and mRNA expression
Anne Karine Lagendijk, Jon D. Moulton, Jeroen Bakkers   +2 more
doaj   +1 more source

Fluorescent in situ hybridization (FISH) on the veterinary diagnostic field

Acta Fytotechnica et Zootechnica, 2016
Proceeding deals with of genomic changes detectable by FISH . The DSD syndrom in Yorkshire terrier 78,XY t (Y-;6p+) was observed by the use of X and Y FISH WCP probes. Following results indicated numerous genomic changes in cancers. Using comparative
Ján Dianovský, Beáta Holečková, Monika Drážovská, Katarína Šivíková, Viera Schwarzbacherová   +4 more
doaj   +1 more source

Distribution of the Primary Endosymbiont (Candidatus Uzinura Diaspidicola) Within Host Insects from the Scale Insect Family Diaspididae

Insects, 2012
It has long been known that armored scale insects harbor endosymbiotic bacteria inside specialized cells called bacteriocytes. Originally, these endosymbionts were thought to be fungal symbionts but they are now known to be bacterial and have been named ...
Katharina Dittmar, Meghan Flarhety, Matthew E. Gruwell   +2 more
doaj   +1 more source