Results 31 to 40 of about 2,818,126 (218)

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

FEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace, Alyssa Borges, Charles Arnaud, Tove Karlsson, Nina K. Bröker, Stefanie Barbirz, Marta Bally   +6 more
wiley   +1 more source

Development and Challenges of Pathogen Molecular Point‐Of‐Care Testing Systems Based on Microfluidic Technology

iLABMED
Molecular point‐of‐care testing (POCT) is characterized by high sensitivity, low reagent demand, fast mixing speed, rapid turnaround time, and ease of use, making it appealing for diagnosing infectious diseases.
Shanshan Ren, Donglai Liu, Sihong Xu
doaj   +1 more source

Development and validation of a national reference material system for quality control of chikungunya virus nucleic acid detection assays

Journal of Clinical Microbiology
The absence of a World Health Organization (WHO) international standard for Chikungunya virus (CHIKV) nucleic acid testing has long impeded the comparability of diagnostic results across different assays and platforms.
Tingting Ma, Lan Zhao, Cong Luo, Yixi Wang, Yabin Tian, Sihong Xu   +5 more
doaj   +1 more source

Identifying miR-431-5p as a promising candidate biomarker for early-onset severe preeclampsia through integrated bioinformatic and clinical analyses: a retrospective study [PDF]

PeerJ
Background Early-onset severe preeclampsia (EOSPE) is a serious pregnancy complication associated with adverse maternal and neonatal outcomes. This study aimed to identify potential diagnostic biomarkers for EOSPE and to investigate their clinical ...
Jianxin Zhang, Hongwei Li, Yu Yan, Xiaoxiao Li, Linlin Zhang, Yangnan Ding, Jun Ma, Enwu Yuan, Xin Zhao   +8 more
doaj   +2 more sources

From mice to humans—divergent strategies for intestinal homeostasis and regeneration

FEBS Letters, EarlyView.
Recent advances such as organoid genome editing, xenotransplantation, imaging, and whole‐genome sequencing have enabled direct studies of human intestinal stem cells (ISCs). These studies reveal species‐specific features, including slower ISC proliferation, distinct injury responses, slower somatic mutation accumulation in humans, and an inverse ...
Keiko Ishikawa, Shinya Sugimoto, Toshiro Sato   +2 more
wiley   +1 more source

Safety of Anti-Tumor Necrosis Factor Therapies in Arthritis Patients

Journal of Pharmacy & Pharmaceutical Sciences, 2014
Purpose. Inflammatory and rheumatic arthritis remain leading causes of disability worldwide. The arthritis therapeutic area commands the largest market for the prescription of biological and non-steroidal anti-inflammatory drugs (NSAID).
Radu M Nanau, Manuela G Neuman
doaj   +1 more source

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Decoding the dynamic extracellular matrix in cancer—3D models and bioscaffolds rewire the rules of tumor progression

FEBS Letters, EarlyView.
Cancer progression is regulated by the dynamic matrix code of the tumor microenvironment, which influences cellular behavior and disease development. Importantly, matrix remodeling in three‐dimensional cancer models more accurately reflects in vivo conditions compared to conventional two‐dimensional systems.
Sylvia Mangani, Konstantinos Spanopoulos, Zoi Piperigkou, Nikos K. Karamanos   +3 more
wiley   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Efficacy and Safety of Platelet Inhibitors

Journal of Pharmacy & Pharmaceutical Sciences, 2013
Ischemic heart disease is the second leading cause of death in the world. The proportion of deaths resulting from this condition has decreased in the last two decades, mainly as a result of improved primary and secondary prevention of cardiovascular ...
Ornella Binazon, Audrey Dubois-Gauche, Radu M Nanau, Manuela G Neuman   +3 more
doaj   +1 more source