Results 41 to 50 of about 120,681,618 (266)

Direct Chemical Interference with the MTT viability assay: Identification and mitigation strategies in New Approach Methodologies (NAMs)

NAM Journal
The reduction of the vital dye 3-(4,5-dimethyl-2- thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) is commonly used to assess the cellular viability and proliferation and thus to evaluate the cytotoxicity of discrete chemicals, complex mixtures, new ...
Courtney Nabua, Megan Creelman, Huang Huang, Nathan R. Wilt, Gertrude-Emilia Costin   +4 more
doaj   +1 more source

Diversity and complexity in neural organoids

FEBS Letters, EarlyView.
Neural organoid research aims to expand genetic diversity on one side and increase tissue complexity on the other. Chimeroids integrate multiple donor genomes within single organoids. Self‐organising multi‐identity organoids, exogenous cell seeding, or enforced assembly of region‐specific organoids contribute to tissue complexity.
Ilaria Chiaradia, Madeline A. Lancaster
wiley   +1 more source

SEQUENCING OF MATK GENE FOR TAXONOMIC IDENTIFICATION AND IN VITRO PROPAGATION OF A LOCAL YAM (DIOSCOREA SP.) CULTIVAR GROWN AT MUONG KHUONG, LAO CAI

, 2018
Khoai mo, local name of Yam species belonging to the Dioscoraceae family, is widely grown at Muong Khuong, Lao Cai province because of their important food and pharmaticeutical valuables.
Bang, Cao Phi, Cao Phi Bang, La Viet Hong, Hong, La Viet   +3 more
core   +1 more source

Determining the toxicological effects of indoor air pollution on both a healthy and an inflammatory-comprised model of the alveolar epithelial barrier in vitro

Particle and Fibre Toxicology
Exposure to indoor air pollutants (IAP) has increased recently, with people spending more time indoors (i.e. homes, offices, schools and transportation).
Kirsty Meldrum, Stephen J. Evans, Michael J. Burgum, Shareen H. Doak, Martin J. D. Clift   +4 more
doaj   +1 more source

Degradation mechanism of the von Willebrand factor A2 domain by nattokinase

FEBS Letters, EarlyView.
Nattokinase, a natto‐derived protease, exhibits potent antithrombotic effects. This study demonstrates that nattokinase directly cleaves the von Willebrand factor (vWF) A2 domain in vitro. Unlike the native regulator ADAMTS13, nattokinase degrades folded vWF independently of shear stress.
Ryuichi Hyakumoto, Masatomo So, Ayako Furukawa, Kenji Sugase   +3 more
wiley   +1 more source

Physical environment in non-ventilated culture vessels affects in vitro growth and morphogenesis of several cultivars of Dianthus Caryophyllus L. [PDF]

, 2002
Differences were shown in the environmental conditions inside four types of non-ventilated culture vessels (glass baby-food jars capped with metal steel or Magenta B-caps, jam glass jars capped with metal steel caps, and Magenta GA7 culture vessels) that
Fall, M. A., Majada Guijo, Juan P., Sánchez Tamés, F.   +2 more
core  

NBI-921352, a first-in-class, NaV1.6 selective, sodium channel inhibitor that prevents seizures in Scn8a gain-of-function mice, and wild-type mice and rats

eLife, 2022
NBI-921352 (formerly XEN901) is a novel sodium channel inhibitor designed to specifically target NaV1.6 channels. Such a molecule provides a precision-medicine approach to target SCN8A-related epilepsy syndromes (SCN8A-RES), where gain-of-function (GoF ...
JP Johnson, Thilo Focken, Kuldip Khakh, Parisa Karimi Tari, Celine Dube, Samuel J Goodchild, Jean-Christophe Andrez, Girish Bankar, David Bogucki, Kristen Burford, Elaine Chang, Sultan Chowdhury, Richard Dean, Gina de Boer, Shannon Decker, Christoph Dehnhardt, Mandy Feng, Wei Gong, Michael Grimwood, Abid Hasan, Angela Hussainkhel, Qi Jia, Stephanie Lee, Jenny Li, Sophia Lin, Andrea Lindgren, Verner Lofstrand, Janette Mezeyova, Rostam Namdari, Karen Nelkenbrecher, Noah Gregory Shuart, Luis Sojo, Shaoyi Sun, Matthew Taron, Matthew Waldbrook, Diana Weeratunge, Steven Wesolowski, Aaron Williams, Michael Wilson, Zhiwei Xie, Rhena Yoo, Clint Young, Alla Zenova, Wei Zhang, Alison J Cutts, Robin P Sherrington, Simon N Pimstone, Raymond Winquist, Charles J Cohen, James R Empfield   +49 more
doaj   +1 more source

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

FEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace, Alyssa Borges, Charles Arnaud, Tove Karlsson, Nina K. Bröker, Stefanie Barbirz, Marta Bally   +6 more
wiley   +1 more source

Mate genetic similarity affects mating behaviour but not maternal investment in mice

Scientific Reports, 2023
Maternal investment can affect the survival and development of offspring. Here we experimentally investigated in mice, whether females alter implantation rates and pup survival after embryo transfer depending on the genetic similarity with their ...
Kerstin E. Auer, Jasmin Primus, Sonja Istel, Maik Dahlhoff, Thomas Rülicke   +4 more
doaj   +1 more source

From mice to humans—divergent strategies for intestinal homeostasis and regeneration

FEBS Letters, EarlyView.
Recent advances such as organoid genome editing, xenotransplantation, imaging, and whole‐genome sequencing have enabled direct studies of human intestinal stem cells (ISCs). These studies reveal species‐specific features, including slower ISC proliferation, distinct injury responses, slower somatic mutation accumulation in humans, and an inverse ...
Keiko Ishikawa, Shinya Sugimoto, Toshiro Sato   +2 more
wiley   +1 more source