Results 51 to 60 of about 120,681,618 (266)

Burden of non-COVID-19 lower respiratory infections in China (1990–2021): a global burden of disease study analysis

Respiratory Research
Background The assessment of lower respiratory infection (LRI) mortality, incidence, and responsible pathogens in China provides a scientific basis for the prevention and management of LRI, especially for evaluating the impact of coronavirus disease 2019
Manyu Li, Zeyu Song, Wenjun Wan, Haiwei Zhou   +3 more
doaj   +1 more source

A robust bacterial high-throughput screening system to evaluate single nucleotide polymorphisms of human homogentisate 1,2-dioxygenase in the context of alkaptonuria

Scientific Reports, 2022
Alkaptonuria (AKU) is a rare inborn error of metabolism caused by a defective homogentisate 1,2-dioxygenase (HGD), an enzyme involved in the tyrosine degradation pathway.
Sien Lequeue, Jessie Neuckermans, Ine Nulmans, Ulrich Schwaneberg, Tamara Vanhaecke, Joery De Kock   +5 more
doaj   +1 more source

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Metabolism of glutathione and ascorbate in lingonberry cultivars during in vitro and ex vitro propagation

, 2013
Lingonberry (Vaccinium vitis-idaea L. ssp. vitis-idaea Britton) cultivars Regal, Splendor, and Erntedank were obtained by conventional softwood cuttings (taken as a control), by in vitro shoot proliferation of node explants, and by adventitious shoot ...
S. C. Debnath, P. Vyas, A. U. Igamberdiev   +2 more
core   +1 more source

Distinct and Dynamic Transcriptome Adaptations of iPSC-Generated Astrocytes after Cytokine Stimulation

Cells, 2022
Astrocytes (ACs) do not only play a role in normal neurogenesis and brain homeostasis, but also in inflammatory and neurodevelopmental disorders. We studied here the different patterns of inflammatory activation triggered by cytokines in human induced ...
Anna-Sophie Spreng, Markus Brüll, Heidrun Leisner, Ilinca Suciu, Marcel Leist   +4 more
doaj   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Supplementation of fetal bovine serum alters histone modification H3R26me2 during preimplantation development of in vitro produced bovine embryos [PDF]

, 2015
In vitro production (IVP) of bovine embryos is not only of great economic importance to the cattle industry, but is also an important model for studying embryo development.
Corrêa, Carolina A.P. [UNESP], Laura L.G. Lorena, João C.T. Penteado, Mingoti, Gisele, Penteado, João C.T., Lorena, Laura L.G., Arnold, Daniel R., Rossi, Guilherme F. [UNESP], Daniel R. Arnold, Guilherme F. Rossi, Flavia L. Lopes, Lopes, Flavia L. [UNESP], Rossi, Guilherme F., Ifran, Aderson M. [UNESP], Carolina A.P. Corrêa, Gaspar, Roberta C., Mingoti, Gisele [UNESP], Gisele Mingoti, Roberta C. Gaspar, Lorena, Laura L.G. [UNESP], Aderson M. Ifran, Corrêa, Carolina A.P., Ifran, Aderson M., Lopes, Flavia L., Penteado, João C.T. [UNESP], Gaspar, Roberta C. [UNESP]   +25 more
core   +1 more source

Development and Challenges of Pathogen Molecular Point‐Of‐Care Testing Systems Based on Microfluidic Technology

iLABMED
Molecular point‐of‐care testing (POCT) is characterized by high sensitivity, low reagent demand, fast mixing speed, rapid turnaround time, and ease of use, making it appealing for diagnosing infectious diseases.
Shanshan Ren, Donglai Liu, Sihong Xu
doaj   +1 more source

Pseudovirus as an Emerging Reference Material in Molecular Diagnostics: Advancement and Perspective

Current Issues in Molecular Biology
In recent years, the persistent emergence of novel infectious pathogens (epitomized by the global coronavirus disease-2019 (COVID-2019) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)) has propelled nucleic acid testing ...
Leiqi Zheng, Sihong Xu
doaj   +1 more source

Microbiome‐blood–brain barrier interactions in aging — mechanisms and therapeutic potential

FEBS Letters, EarlyView.
Aging reshapes the gut microbiome (↓SCFA‐producing commensals; ↑pro‐inflammatory outputs), shifting circulating metabolites (↓SCFAs; ↑LPS, ↑TMAO, ↑PAA) that act at the BBB to increase nonspecific transcytosis, alter transport, and promote astrocyte reactivity, heightening brain vulnerability.
Daniel Cuervo‐Zanatta, Hari Baskar Balasubramanian, Amir Pasokh, Marietta Zille   +3 more
wiley   +1 more source