Results 51 to 60 of about 3,958,292 (318)

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

Development and Challenges of Pathogen Molecular Point‐Of‐Care Testing Systems Based on Microfluidic Technology

iLABMED
Molecular point‐of‐care testing (POCT) is characterized by high sensitivity, low reagent demand, fast mixing speed, rapid turnaround time, and ease of use, making it appealing for diagnosing infectious diseases.
Shanshan Ren, Donglai Liu, Sihong Xu
doaj   +1 more source

Pseudovirus as an Emerging Reference Material in Molecular Diagnostics: Advancement and Perspective

Current Issues in Molecular Biology
In recent years, the persistent emergence of novel infectious pathogens (epitomized by the global coronavirus disease-2019 (COVID-2019) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)) has propelled nucleic acid testing ...
Leiqi Zheng, Sihong Xu
doaj   +1 more source

Phosphatidylinositol 4‐kinase as a target of pathogens—friend or foe?

FEBS Letters, EarlyView.
This graphical summary illustrates the roles of phosphatidylinositol 4‐kinases (PI4Ks). PI4Ks regulate key cellular processes and can be hijacked by pathogens, such as viruses, bacteria and parasites, to support their intracellular replication. Their dual role as essential host enzymes and pathogen cofactors makes them promising drug targets.
Ana C. Mendes, Guilherme M. Azevedo, Amanda P. Barcellos, Diana Bahia   +3 more
wiley   +1 more source

Oxygen tension affects histone remodeling of in vitro-produced embryos in a bovine model [PDF]

, 2015
In vitro production of bovine embryos is a biotechnology of great economic impact. Epigenetic processes, such as histone remodeling, control gene expression and are essential for proper embryo development.
Gaspar, Roberta C., Garcia, Joaquim M., Vantini, Roberta [UNESP], Penteado, Joao C. T. [UNESP], Rocha, Carlos V. da, Correa, Carolina A. P., del Collado, Maite, Vantini, Roberta, Correa, Carolina A. P. [UNESP], del Collado, Maite [UNESP], Arnold, Daniel R., Lopes, Flavia L., Rocha, Carlos V. da [UNESP], Lopes, Flavia L. [UNESP], Penteado, Joao C. T., Gaspar, Roberta C. [UNESP], Garcia, Joaquim M. [UNESP]   +16 more
core   +1 more source

Development and validation of a national reference material system for quality control of chikungunya virus nucleic acid detection assays

Journal of Clinical Microbiology
The absence of a World Health Organization (WHO) international standard for Chikungunya virus (CHIKV) nucleic acid testing has long impeded the comparability of diagnostic results across different assays and platforms.
Tingting Ma, Lan Zhao, Cong Luo, Yixi Wang, Yabin Tian, Sihong Xu   +5 more
doaj   +1 more source

Safety of Anti-Tumor Necrosis Factor Therapies in Arthritis Patients

Journal of Pharmacy & Pharmaceutical Sciences, 2014
Purpose. Inflammatory and rheumatic arthritis remain leading causes of disability worldwide. The arthritis therapeutic area commands the largest market for the prescription of biological and non-steroidal anti-inflammatory drugs (NSAID).
Radu M Nanau, Manuela G Neuman
doaj   +1 more source

Organogenesis in vitro

Current Opinion in Cell Biology, 2021
Organoids are three-dimensional structures that self-organize from human pluripotent stem cells or primary tissue, potentially serving as a traceable and manipulatable platform to facilitate our understanding of organogenesis. Despite the ongoing advancement in generating organoids of diverse systems, biological applications of in vitro generated ...
Kentaro Iwasawa, Takanori Takebe
openaire   +2 more sources

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source

The impact of bisphenol AF on skeletal muscle function and differentiation in vitro [PDF]

Various environmental chemicals have been identified as contributors to metabolic diseases. Bisphenol AF (BPAF), a substitute for bisphenol A, has been associated with changes in glucose metabolism and incidence of type 2 diabetes mellitus in humans ...
Munic Kos, Vesna,, Tzioufa, Foteini, Westerblad, Håkan, Westerblad, H,, Bruton, Joseph,, Cui, M,, Cui, Minying, Cui, Minying,, Bruton, J,, Tzioufa, F, Bruton, Joseph, Tzioufa, Foteini,, Westerblad, Håkan,, Kos, VM, Munic Kos, Vesna   +14 more
core   +1 more source