Results 261 to 270 of about 633,020 (302)
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In vitro expansion of hematopoietic stem cells
Stem Cells, 1997We have investigated the effects of interleukin 3 (IL-3) and IL-1 on in vitro expansion of murine hematopoietic progenitors and stem cells using a highly purified progenitor population. Lineage negative, Ly-6A/E+, c-kit+ bone marrow cells from male mice were cultured in suspension in the presence of stem cell factor, IL-6, IL-11 and erythropoietin with
M, Ogawa, Y, Yonemura, H, Ku
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Biomechanical properties of skin in vitro for different expansion methods
Clinical Biomechanics, 2004In plastic surgery, clinicians are interested in replacing conventional expansion by rapid expansion, because the expansion period could be shortened greatly. Also people are concerned that skin properties after rapid expansion might not approach those after conventional expansion.Biomechanical testing of skin for different expansion methods.It would ...
Yan-Jun Zeng
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In vitro expansion of Vγ9Vδ2 T cells for immunotherapy
2020Vγ9Vδ2 T cells can exhibit potent anti-tumor activity and have gained much attention as an interesting tool for cancer immunotherapy by adoptive cell transfer. In this chapter, we explain the different approaches for the in vitro expansion of Vγ9Vδ2 T cells for their subsequent adoptive transfer and present a detailed protocol for the successful in ...
Christian, Peters +4 more
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Isolation and in vitro expansion of porcine spermatogonial stem cells
Reproduction in Domestic Animals, 2021AbstractSpermatogonial stem cells (SSCs) are the only adult stem cells capable of passing genetic information to offspring through their ability to both self‐renew and differentiate into mature spermatozoa. SSCs can be transplanted to establish donor‐derived spermatogenesis in recipient animals, thus offering a novel reproductive tool for ...
Xin Zhao +6 more
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In Vitro Expansion of Corneal Endothelial Cells for Transplantation
2020The corneal endothelium forms a leaky barrier between the corneal stroma and the aqueous humor of the anterior chamber. This cell monolayer maintains the corneal stroma in a state of relative dehydration, a process called deturgescence, which is required in order to obtain corneal stromal transparency. Endothelial dysfunctions lead to visual impairment
Kim, Santerre +3 more
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In vitro expansion patterns of ameroid ring constrictors
American Journal of Veterinary Research, 2008Abstract Objective—To evaluate and quantify in vitro dimensional changes of ameroid ring constrictors (ARCs) with and without the outer stainless steel ring in place over time. Sample Population—12 ARCs (5.0-mm diameter). Procedures—6 ARCs were immersed in canine plasma baths for 34 days without the stainless steel outer ring in place (group N), and 6 ...
Steven S, Leshem +2 more
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Purification and expansion of human Schwann cells in vitro
Nature Medicine, 1995The ability to culture cells from the human nervous system provides new insight into the pathophysiology of neurological diseases and could be crucial to the development of gene replacement therapies and neural transplantation. We report that the proliferation of human Schwann cells isolated from paediatric and adult nerves is sustained in vitro by ...
J L, Rutkowski +3 more
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In Vitro Expansion and Transplantation of Intestinal Crypt Stem Cells
Gastroenterology, 2012Adult tissue stem cells hold great promise for regenerative medicine strategies. Major hurdles in their clinical application persist and include the identification of such stem cells, their isolation and in vitro expansion, and—finally—their transplantation. These hurdles have recently been overcome for stem cells of the intestinal tract.
Li, V.S., Clevers, H.
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In Vitro Expansion of Hematopoietic Cells for Clinical Application
1995The past 15 years have seen a tremendous explosion in our knowledge of the molecular and cellular regulation of hematopoietic differentiation. Launched with the identification and purification of candidate hematopoietic stem cells by Visser et al. in 1984 [1] and the molecular cloning of recombinant human (rH-) granulocyte macrophage-colony stimulating
Stephen G. Emerson +11 more
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In Vitro Expansion of Tissue Cells by Conditional Proliferation
2007Cell therapies rely on the implantation of well-characterized functional cells with defined properties. Often, the cells of interest do not proliferate in vitro and thus cannot be expanded to the amount needed for characterization, purification, manipulation, or cloning.
Tobias, May +2 more
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