Results 21 to 30 of about 670,418 (378)

Ketamine intervention limits pathogen expansion in vitro [PDF]

Pathogens and Disease, 2018
Ketamine is one of several clinically important drugs whose therapeutic efficacy is due in part to their ability to act upon ion channels prevalent in nearly all biological systems. In studying eukaryotic and prokaryotic organisms in vitro, we show that ketamine short-circuits the growth and spatial expansion of three microorganisms, Stachybotrys ...
German Torres, Christopher L Hoehmann, Joshua A Cuoco, Kyle Hitscherich, Charles Pavia, Michael Hadjiargyrou, Joerg R Leheste   +6 more
openaire   +2 more sources

Agent based modelling helps in understanding the rules by which fibroblasts support keratinocyte colony formation [PDF]

, 2008
Background: Autologous keratincoytes are routinely expanded using irradiated mouse fibroblasts and bovine serum for clinical use. With growing concerns about the safety of these xenobiotic materials, it is desirable to culture keratinocytes in media ...
A Macieira-Coelho, A Szabowski, AJ Bogaerdt, AJ Bullock, BM Kim, BS Croix, C Clavel, CA Harrison, CC Chang, CG Tepper, CM Nelson, CS Osborne, D Drasdo, D Morel, E Kolettas, EAG Blomme, ER Waelti, Eshel Ben-Jacob, F Goulet, F Takeuchi, FA Meineke, FY Bhora, G Hubner, GG Gallico, GTB Casella, H Smola, H Uchi, HJ Stark, HJ Wearing, I Scheffrahn, I Sher, IR Williams, J Ashkenas, J Gailit, J Galle, J Grossmann, J Kopp, J Rashbass, JC Adams, JG Rheinwald, JL Walker, JM Ponciano, JM Sorrell, JS Rubin, JV Gorelik, K Orford, K Suzuki, L Mishra, LY Zhang, M Absher, M Okazaki, M Sakaguchi, M Sibilia, MC Higham, Mike Holcombe, MN Szabowski, MR Owen, MT Santini, N Grabe, N Matsuyoshi, NT Dai, P Angel, Phil McMinn, R Fleischmajer, R Roguet, RA Dawson, RK Assoian, Rod Smallwood, RP Witte, S Coakley, S Kansra, S MacNeil, S Tagashira, S Werner, Sheila MacNeil, SN Bhatia, T Sun, T Sun, T Sun, T Sun, TA Beyer, Tao Sun, TB Gizelda, TK Dilley, U Keller, U Mirastschijski, UB Jensen, W Bowers, W Sabine, Y Kanzaki, Y Yamaguchi   +90 more
core   +12 more sources

Notch pathway activation enhances cardiosphere in vitro expansion. [PDF]

J Cell Mol Med, 2018
AbstractCardiospheres (CSps) are self‐assembling clusters of a heterogeneous population of poorly differentiated cells outgrowing from in vitro cultured cardiac explants. Scanty information is available on the molecular pathways regulating CSp growth and their differentiation potential towards cardiac and vascular lineages.
Secco I, Barile L, Torrini C, Zentilin L, Vassalli G, Giacca M, Collesi C.   +6 more
europepmc   +8 more sources

Morphokinetics of human blastocyst expansion in vitro [PDF]

Reproductive BioMedicine Online, 2016
Time-lapse imaging offers new tools to study dynamic processes of development such as blastocyst formation and expansion. This study quantitatively describes expansion in human blastocysts from donated oocytes. Measurements of hourly interval rate of changes in the blastocoel cross-sectional area revealed oscillatory pulses having 2-4 h periodicities ...
T T F, Huang, K, Chinn, T, Kosasa, H J, Ahn, B, Kessel   +4 more
openaire   +2 more sources

In vitro expansion of human sperm through nuclear transfer. [PDF]

Cell Res, 2020
Zhang XM, Wu K, Zheng Y, Zhao H, Gao J, Hou Z, Zhang M, Liao J, Zhang J, Gao Y, Li Y, Li L, Tang F, Chen ZJ, Li J.   +14 more
europepmc   +4 more sources

Expansion of Human Embryonic Stem Cells In Vitro [PDF]

Current Protocols in Stem Cell Biology, 2007
AbstractThis unit describes a protocol for the large‐scale expansion of karyotypically normal human embryonic stem cells (hESCs). hESCs can be maintained indefinitely as dense colonies that are mechanically cut into pieces, which are subsequently transferred to fresh organ culture dishes seeded with primary mouse embryonic fibroblasts (MEFs). hESCs can
Magdaline, Costa, Koula, Sourris, Tanya, Hatzistavrou, Andrew G, Elefanty, Edouard G, Stanley   +4 more
openaire   +2 more sources

The Calcium-Sensing Receptor Is Involved in Follicle-Stimulating Hormone-Induced Cumulus Expansion in in vitro Cultured Porcine Cumulus-Oocyte Complexes

Frontiers in Cell and Developmental Biology, 2021
The Calcium-Sensing Receptor (CASR) is a G protein-coupled receptor of the C family that reportedly promotes maturation of porcine oocytes. However, its role in cumulus expansion of cumulus-oocyte complexes (COCs) is not well known.
Huage Liu, Huage Liu, Dan Zhou, Cong Liu, Qingrui Zhuan, Yan Luo, Xianhong Mo, Xiangwei Fu, Yunpeng Hou   +8 more
doaj   +1 more source

Phthalates affect the in vitro expansion of human hematopoietic stem cell. [PDF]

Cytotechnology, 2019
Gutiérrez-García AK, Flores-Kelly JM, Ortiz-Rodríguez T, Kalixto-Sánchez MA, De León-Rodríguez A.   +4 more
europepmc   +3 more sources

A passage-dependent network for estimating the in vitro senescence of mesenchymal stromal/stem cells using microarray, bulk and single cell RNA sequencing

Frontiers in Cell and Developmental Biology, 2023
Long-term in vitro culture of human mesenchymal stem cells (MSCs) leads to cell lifespan shortening and growth stagnation due to cell senescence. Here, using sequencing data generated in the public domain, we have established a specific regulatory ...
Yong Yang, Wencheng Zhang, Wencheng Zhang, Wencheng Zhang, Xicheng Wang, Xicheng Wang, Xicheng Wang, Jingxian Yang, Yangyang Cui, Yangyang Cui, Yangyang Cui, Yangyang Cui, Haimeng Song, Haimeng Song, Haimeng Song, Weiping Li, Wei Li, Le Wu, Yao Du, Zhiying He, Zhiying He, Zhiying He, Jun Shi, Jiangnan Zhang   +23 more
doaj   +1 more source

Identification and In Vitro Expansion of Buccal Epithelial Cells. [PDF]

Cell Transplant, 2018
Ex vivo-expanded buccal mucosal epithelial (BME) cell transplantation has been used to reconstruct the ocular surface. Methods for enrichment and maintenance of BME progenitor cells in ex vivo cultures may improve the outcome of BME cell transplantation. However, the parameter of cell seeding density in this context has largely been neglected.
Ghaemi SR, Delalat B, Harding FJ, Irani YD, Williams KA, Voelcker NH.   +5 more
europepmc   +6 more sources