Results 11 to 20 of about 633,020 (302)

Progression in the In Vitro Macrophage Expansion

Journal of Immunology Research
Macrophages play essential roles in homeostasis and disease, and they were considered terminally differentiated cells that cannot proliferate. However, growing evidence shows that macrophages can self-renew in homeostasis and multiple pathological states
Yunpeng Wei, Jingzhao Yang, Wenhong Zu, Mengran Wang, Yong Zhao   +4 more
doaj   +3 more sources

IL6 supports long-term expansion of hepatocytes in vitro

Nature Communications, 2022
Hepatocytes are very difficult to expand in vitro. Here the authors discover that IL6 promotes long-term expansion (>30 passages) of primary mouse hepatocytes in vitro by converting the cells into hepatic progenitor cells, which maintain full capacity of
Ren Guo, Mengmeng Jiang, Gang Wang, Bing Li, Xiaohui Jia, Yan Ai, Shanshan Chen, Peilan Tang, Aijie Liu, Qianting Yuan, Xin Xie   +10 more
doaj   +3 more sources

In vitro expansion of fetal liver hematopoietic stem cells. [PDF]

Sci Rep, 2021
AbstractFetal liver hematopoietic stem and progenitor cells (HSPCs) have been considered appropriate for the management of aplastic anemia owing to their proliferative potential. Bone marrow recovery was possible in some cases; the engraftment potential of these cells, however was unsatisfactory, possibly due to the availability of a smaller number of ...
Bhardwaj R, Kumar L, Chhabra D, Mehra NK, Sharma A, Mohanty S, Kochupillai V.   +6 more
europepmc   +6 more sources

Identification and In Vitro Expansion of Buccal Epithelial Cells. [PDF]

Cell Transplant, 2018
Ex vivo-expanded buccal mucosal epithelial (BME) cell transplantation has been used to reconstruct the ocular surface. Methods for enrichment and maintenance of BME progenitor cells in ex vivo cultures may improve the outcome of BME cell transplantation. However, the parameter of cell seeding density in this context has largely been neglected.
Ghaemi SR, Delalat B, Harding FJ, Irani YD, Williams KA, Voelcker NH.   +5 more
europepmc   +6 more sources

Morphokinetics of human blastocyst expansion in vitro [PDF]

Reproductive BioMedicine Online, 2016
Time-lapse imaging offers new tools to study dynamic processes of development such as blastocyst formation and expansion. This study quantitatively describes expansion in human blastocysts from donated oocytes. Measurements of hourly interval rate of changes in the blastocoel cross-sectional area revealed oscillatory pulses having 2-4 h periodicities ...
T T F, Huang, K, Chinn, T, Kosasa, H J, Ahn, B, Kessel   +4 more
openaire   +2 more sources

In Vitro Expansion of GGC:GCC Repeats: Identification of the Preferred Strand of Expansion [PDF]

Nucleic Acids Research, 1996
The human fragile-X syndrome, a major cause of inherited mental retardation, is associated with expansion of the trinucleotide repeat GGC:GCC. Repetitive sequences in DNA are subject to slippage during catalysis by DNA polymerases. We characterized the extent of slippage of synthetic GGC:GCC repeats by various DNA polymerases: Taq DNA polymerase ...
J, Ji, N J, Clegg, K R, Peterson, A L, Jackson, C D, Laird, L A, Loeb   +5 more
openaire   +2 more sources

A new technique for primary hepatocyte expansion in vitro [PDF]

Biotechnology and Bioengineering, 2008
AbstractThe current application for many potential cell‐based treatments for liver failure is limited by the low availability of mature functional hepatocytes. Although adult hepatocytes have a remarkable ability to proliferate in vivo, attempts to proliferate adult hepatocytes in vitro have been less successful.
Cheul H, Cho, François, Berthiaume, Arno W, Tilles, Martin L, Yarmush   +3 more
openaire   +2 more sources

Ketamine intervention limits pathogen expansion in vitro [PDF]

Pathogens and Disease, 2018
Ketamine is one of several clinically important drugs whose therapeutic efficacy is due in part to their ability to act upon ion channels prevalent in nearly all biological systems. In studying eukaryotic and prokaryotic organisms in vitro, we show that ketamine short-circuits the growth and spatial expansion of three microorganisms, Stachybotrys ...
German Torres, Christopher L Hoehmann, Joshua A Cuoco, Kyle Hitscherich, Charles Pavia, Michael Hadjiargyrou, Joerg R Leheste   +6 more
openaire   +2 more sources

Expansion of Human Embryonic Stem Cells In Vitro [PDF]

Current Protocols in Stem Cell Biology, 2007
AbstractThis unit describes a protocol for the large‐scale expansion of karyotypically normal human embryonic stem cells (hESCs). hESCs can be maintained indefinitely as dense colonies that are mechanically cut into pieces, which are subsequently transferred to fresh organ culture dishes seeded with primary mouse embryonic fibroblasts (MEFs). hESCs can
Magdaline, Costa, Koula, Sourris, Tanya, Hatzistavrou, Andrew G, Elefanty, Edouard G, Stanley   +4 more
openaire   +2 more sources

In vitro and in vivo expansion of hematopoietic stem cells [PDF]

Oncogene, 2004
The capacity for sustained self-renewal--the generation of daughter cells having the same regenerative properties as the parent cell--is the defining feature of hematopoietic stem cells (HSCs). Strong evidence exists that self-renewal of HSC is under extrinsic biological control in vivo.
Guy, Sauvageau, Norman N, Iscove, R Keith, Humphries   +2 more
openaire   +2 more sources