Results 11 to 20 of about 365,110 (263)
Progression in the In Vitro Macrophage Expansion
Journal of Immunology ResearchMacrophages play essential roles in homeostasis and disease, and they were considered terminally differentiated cells that cannot proliferate. However, growing evidence shows that macrophages can self-renew in homeostasis and multiple pathological states
Yunpeng Wei +4 more
doaj +3 more sources
In vitro expansion of fetal liver hematopoietic stem cells. [PDF]
Sci Rep, 2021 AbstractFetal liver hematopoietic stem and progenitor cells (HSPCs) have been considered appropriate for the management of aplastic anemia owing to their proliferative potential. Bone marrow recovery was possible in some cases; the engraftment potential of these cells, however was unsatisfactory, possibly due to the availability of a smaller number of ...
Bhardwaj R +6 more
europepmc +6 more sources
Morphokinetics of human blastocyst expansion in vitro [PDF]
Reproductive BioMedicine Online, 2016 Time-lapse imaging offers new tools to study dynamic processes of development such as blastocyst formation and expansion. This study quantitatively describes expansion in human blastocysts from donated oocytes. Measurements of hourly interval rate of changes in the blastocoel cross-sectional area revealed oscillatory pulses having 2-4 h periodicities ...
T T F, Huang +4 more
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In Vitro Expansion of GGC:GCC Repeats: Identification of the Preferred Strand of Expansion [PDF]
Nucleic Acids Research, 1996 The human fragile-X syndrome, a major cause of inherited mental retardation, is associated with expansion of the trinucleotide repeat GGC:GCC. Repetitive sequences in DNA are subject to slippage during catalysis by DNA polymerases. We characterized the extent of slippage of synthetic GGC:GCC repeats by various DNA polymerases: Taq DNA polymerase ...
J, Ji +5 more
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A new technique for primary hepatocyte expansion in vitro [PDF]
Biotechnology and Bioengineering, 2008 AbstractThe current application for many potential cell‐based treatments for liver failure is limited by the low availability of mature functional hepatocytes. Although adult hepatocytes have a remarkable ability to proliferate in vivo, attempts to proliferate adult hepatocytes in vitro have been less successful.
Cheul H, Cho +3 more
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Ketamine intervention limits pathogen expansion in vitro [PDF]
Pathogens and Disease, 2018 Ketamine is one of several clinically important drugs whose therapeutic efficacy is due in part to their ability to act upon ion channels prevalent in nearly all biological systems. In studying eukaryotic and prokaryotic organisms in vitro, we show that ketamine short-circuits the growth and spatial expansion of three microorganisms, Stachybotrys ...
German Torres +6 more
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Expansion of Human Embryonic Stem Cells In Vitro [PDF]
Current Protocols in Stem Cell Biology, 2007 AbstractThis unit describes a protocol for the large‐scale expansion of karyotypically normal human embryonic stem cells (hESCs). hESCs can be maintained indefinitely as dense colonies that are mechanically cut into pieces, which are subsequently transferred to fresh organ culture dishes seeded with primary mouse embryonic fibroblasts (MEFs). hESCs can
Magdaline, Costa +4 more
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In vitro and in vivo expansion of hematopoietic stem cells [PDF]
Oncogene, 2004 The capacity for sustained self-renewal--the generation of daughter cells having the same regenerative properties as the parent cell--is the defining feature of hematopoietic stem cells (HSCs). Strong evidence exists that self-renewal of HSC is under extrinsic biological control in vivo.
Guy, Sauvageau +2 more
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In vitro expansion of human beta cells [PDF]
Diabetologia, 2008 AbbreviationGFP green fluorescent proteinTo the Editor: Expansion of beta cells from isolated adulthuman islets in tissue culture represents an attractivepotential source of cells for cell therapy for diabetes.Evidence supports the regeneration of adult mouse betacells in vivo from both insulin-producing cells [1, 2] andpancreatic duct cells [3], and ...
openaire +2 more sources
Management of recurrent postoperative fungal endophthalmitis
Indian Journal of Ophthalmology, 2014 Aim: To report the management of recurrent postoperative fungal endophthalmitis (POFE) after failed pars plana vitrectomy (PPV) and antifungal therapy. Settings and Design: Tertiary Care Referral Centre in North India.
Anand Vinekar +5 more
doaj +1 more source