In vitro expansion of human beta cells [PDF]
AbbreviationGFP green fluorescent proteinTo the Editor: Expansion of beta cells from isolated adulthuman islets in tissue culture represents an attractivepotential source of cells for cell therapy for diabetes.Evidence supports the regeneration of adult mouse betacells in vivo from both insulin-producing cells [1, 2] andpancreatic duct cells [3], and ...
openaire +2 more sources
Human bone marrow mesenchymal progenitors: perspectives on an optimized in vitro manipulation
When it comes to regenerative medicine, mesenchymal stem cells (MSCs) are considered one of the most promising cell types for use in many cell therapies and bioengineering protocols.
Eric Cordeiro-Spinetti +7 more
doaj +1 more source
In vitro expansion of human sperm through nuclear transfer. [PDF]
Zhang XM +14 more
europepmc +4 more sources
An optimized method for isolating and expanding invariant natural killer T cells from mouse spleen [PDF]
The ability to rapidly secrete cytokines upon stimulation is a functional characteristic of the invariant natural killer T (iNKT) cell lineage. iNKT cells are therefore characterized as an innate T cell population capable of activating and steering ...
Drennan, Michael +2 more
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Purpose: To analyze the complications and their managements in Descemet′s stripping endothelial keratoplasty (DSEK) in consecutive 430 cases by single surgeon in a tertiary eye hospital.
Samar K Basak, Soham Basak
doaj +1 more source
Hypoxic Culture Conditions as a Solution for Mesenchymal Stem Cell Based Regenerative Therapy [PDF]
Cell-based regenerative therapies, based on in vitro propagation of stem cells, offer tremendous hope to many individuals suffering from degenerative diseases that were previously deemed untreatable.
Abu Kasim, N. H. +3 more
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Optimization of the Expansion and Differentiation of Rabbit Chondrocytes In Vitro [PDF]
Objective: To develop a tissue culture expansion method for rabbit chondrocytes that promotes robust expansion while preserving chondrogenic potential. Design: Rabbit chondrocytes isolated from articular or auricular chondrocytes were ...
Mounts, T. +3 more
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Context: Children with learning disabilities might have difficulties to communicate effectively and give reliable responses as required in various visual function testing procedures.
Nurul Farhana Abu Bakar, Ai-Hong Chen
doaj +1 more source
Frequency analysis of cytolytic T cell precursors (CTL-P) generated in vivo during lethal rabies infection of mice. I. Distinction of CTL-P with different interleukin 2 sensitivity [PDF]
The aim of this study was to determine the number and state of activity of cytolytic T lymphocytes (CTL) and their precursors (CTL-P) present in vivo during the early stages of viral infection. The local response to lethal infection with rabies virus was
Cerottini +21 more
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IL-15 augments TCR-induced CD4⁺ T cell expansion in vitro by inhibiting the suppressive function of CD25High CD4⁺ T cells [PDF]
Due to its critical role in NK cell differentiation and CD8(+) T cell homeostasis, the importance of IL-15 is more firmly established for cytolytic effectors of the immune system than for CD4(+) T cells. The increased levels of IL-15 found in several CD4(
Boonefaes, Tom +5 more
core +6 more sources

