Results 41 to 50 of about 365,110 (263)

Aggressive posterior retinopathy of prematurity in infants ≥1500 g birth weight

Indian Journal of Ophthalmology, 2014
In this retrospective case series, we report the spectrum and outcomes of aggressive posterior retinopathy of prematurity (APROP) in infants ≥1500 g birth weight. Twenty-nine eyes of 15 infants are included.
Gaurav Sanghi, Mangat R Dogra, Deeksha Katoch, Amod Gupta   +3 more
doaj   +1 more source

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

FEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace, Alyssa Borges, Charles Arnaud, Tove Karlsson, Nina K. Bröker, Stefanie Barbirz, Marta Bally   +6 more
wiley   +1 more source

In vitro skin expansion: Wound healing assessment

Wound Repair and Regeneration, 2017
ABSTRACTFor treatments requiring split‐thickness skin grafts, it is preferable to mesh the grafts. This reduces the amount of excised skin and covers more wound area. The mesh technique, however, destroys surface continuity, which results in scarring. Strain‐based bioreactors, on the other hand, have successfully expanded split‐thickness skin grafts in
Peter M, Prim, Han Su, Kim, Lindsey E, Shapiro, Jae Sung, Lee, James H, Kaan, John D, Jackson, James J, Yoo, Anthony, Atala, Sang Jin, Lee   +8 more
openaire   +3 more sources

In vitro expansion affects the response of chondrocytes to mechanical stimulation [PDF]

Osteoarthritis and Cartilage, 2008
Expansion of autologous chondrocytes is a common step in procedures for cartilage defect repair. Subsequent dedifferentiation can alter cellular response to mechanical loading, having major consequences for the cell's behavior in vivo after reimplantation.
Das, RHJ, Jahr, Holger, Verhaar, Jan, van der Linden, JC (Jacqueline), van Osch, Gerjo, Weinans, HH   +5 more
openaire   +3 more sources

Evolution of ASC Immunophenotypical Subsets During Expansion In Vitro [PDF]

International Journal of Molecular Sciences, 2020
Adipose-derived stromal/stem cells (ASCs) are currently being considered for clinical use for a number of indications. In order to develop standardized clinical protocols, it is paramount to have a full characterization of the stem cell preparations. The surface marker expression of ASCs has previously been characterized in multiple studies.
Qiuyue Peng, Hiva Alipour, Simone Porsborg, Trine Fink, Vladimir Zachar   +4 more
openaire   +3 more sources

Successful transportation of human corneal endothelial tissues without cool preservation in varying Indian tropical climatic conditions and in vitro cell expansion using a novel polymer

Indian Journal of Ophthalmology, 2014
Background: Though the transplantation of human corneal endothelial tissue (CET) separated from cadaver cornea is in practice, its transportation has not been reported.
Srinivas K Rao, John Sudhakar, Periyasamy Parikumar, Sundaram Natarajan, Aditya Insaan, Hiroshi Yoshioka, Yuichi Mori, Shigeo Tsukahara, Subramani Baskar, Sadananda Rao Manjunath, Rajappa Senthilkumar, Paramasivam Thamaraikannan, Thangavelu Srinivasan, Senthilkumar Preethy, Samuel J K Abraham   +14 more
doaj   +1 more source

Expansion Improved the Physical and Chemical Properties and In Vitro Rumen Digestibility of Buckwheat Straw

Animals, 2023
The hard texture and poor palatability of straw are important factors that hinder its application in feed. Expansion is a technology that can improve the utilization of biomass, but few studies have comprehensively revealed how to change physicochemical ...
Xiaohui Cao, Sasa Zuo, Yanli Lin, Rui Cai, Fuyu Yang, Xuekai Wang, Chuncheng Xu   +6 more
doaj   +1 more source

From mice to humans—divergent strategies for intestinal homeostasis and regeneration

FEBS Letters, EarlyView.
Recent advances such as organoid genome editing, xenotransplantation, imaging, and whole‐genome sequencing have enabled direct studies of human intestinal stem cells (ISCs). These studies reveal species‐specific features, including slower ISC proliferation, distinct injury responses, slower somatic mutation accumulation in humans, and an inverse ...
Keiko Ishikawa, Shinya Sugimoto, Toshiro Sato   +2 more
wiley   +1 more source

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

In Vitro Isolation and Expansion of Human Retinal Progenitor Cells [PDF]

Experimental Neurology, 2002
Human retinal development proceeds with temporal and spatial precision. Although differentiation starts around the beginning of the third month of gestation, the majority of cells in the outer neuroblastic layer of human neural retina are still proliferating, as evidenced by their Ki-67 immunoreactivity.
Yang, Peng, Seiler, Magdalene J, Aramant, Robert B, Whittemore, Scott R   +3 more
openaire   +4 more sources