Results 61 to 70 of about 118,389,530 (261)
Semina: Ciências Exatas e Tecnológicas, 2003 The evaluation of a culture medium for the in vitro culture of a species is performed using its physical and/or chemical properties. However, the analysis of the experimental results makes it possible to evaluate its quality.
Daniel Furtado Ferreira +2 more
doaj
Liver organoids: modelling complexity in homeostasis and disease
FEBS Letters, EarlyView.Studying liver in vitro has been challenging because simple 2D cell cultures fail to capture liver's cellular and architectural complexity. To bridge this gap, scientists increasingly use organoids, 3D liver models which better mimic liver composition and function. This review examines recent advances in liver organoid complexity and realism, discusses
Anna M. Dowbaj, Meritxell Huch
wiley +1 more source
The microbiome in human skin aging
FEBS Letters, EarlyView.Age‐related skin changes encompass the well‐known visible phenotypic alterations, together with microbiome dysbiosis and a series of molecular aging hallmarks. These hallmarks characterize not only a fully stablished aged phenotype but also the skin aging process itself.
Manuel Huerta Arana +3 more
wiley +1 more source
An in vitro Model of Human Retinal Detachment Reveals Successive Death Pathway Activations
Frontiers in Neuroscience, 2020 Purposewas to create an in vitro model of human retinal detachment (RD) to study the mechanisms of photoreceptor death.MethodsHuman retinas were obtained through eye globe donations for research purposes and cultivated as explants.
Jelena Potic +13 more
doaj +1 more source
FEBS Letters, EarlyView.This review focuses on the role of autophagy and mitophagy in maintaining pancreatic β‐cell function and homeostasis. We discuss how genetic defects affecting these pathways contribute to the development of type 1, type 2, monogenic, and gestational diabetes. We further explore their potential as therapeutic targets. Created in BioRender.
Yunkyeong Lee +2 more
wiley +1 more source
FEBS Letters, EarlyView.Encapsulins are protein nanocompartments that play an important role in iron storage. In the Myxococcus xanthus encapsulin system, two cargo proteins called EncB and EncC contribute to iron mineralization. Here, we show that EncB and EncC generate iron‐containing minerals with distinct chemical compositions, suggesting that the composition of stored ...
Harry B. McDowell +2 more
wiley +1 more source
Structural and biochemical analysis of a B12 superbinder
FEBS Letters, EarlyView.BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices +3 more
wiley +1 more source
The use of an in vitro cornea for predicting ocular toxicity
, 2022 S.309-318 : Ill., Lit.This paper presents the development of an in vitro cornea model to assess ocular irritancy. The in vitro cornea is an organotypic culture composed of corneal stromal fibroblasts embedded in a matrix of collagen with an underlying ...
Maier-Reif, K. +2 more
core +1 more source
Computational and Structural Biotechnology JournalThis study proposes a novel in vitro–in vivo extrapolation (IVIVE) strategy that integrates a patented biomimetic in vitro system with pharmacokinetic modeling to improve the prediction of in vivo drug kinetics.
Seongwon Park +6 more
doaj +1 more source
Pharmaceutics, 2020 Periodontal therapy using antimicrobials that are topically applied requires slow or controlled release devices. The in vitro antimicrobial activity of biodegradable polymer formulations that contain a new minocycline lipid complex (P-MLC) was evaluated.
Jan-Luca Schmid +7 more
doaj +1 more source