Results 191 to 200 of about 114,054,514 (226)

Mouse In Vitro Spermatogenesis on 3D Bioprinted Scaffolds

Methods in Molecular Biology
Testes have a complex architecture that is compartmentalized into seminiferous tubules with a diameter of approximatively 200 μm in which the germ cells differentiate, surrounded by a basement membrane and interstitium. 3D bioprinting might be used to recreate the compartmentalized testicular architecture in vitro.
Ellen Goossens, Tamara Vanhaecke, Yoni Baert   +2 more
exaly   +3 more sources

Restoration of fertility by in-vitro spermatogenesis

Lancet, The, 1999
Ermanno Greco, Carmen Mendoza, Mustafa Bahceci   +2 more
exaly   +3 more sources

Prospects for spermatogenesis in vitro

Theriogenology, 2003
In recent years, extraordinary progress has been made in a broad range of reproductive technologies, including spermatogonial transplantation in the male. However, effective procedures for the complete recapitulation of spermatogenesis in vitro, including meiosis, have remained elusive.
J E, Parks, D R, Lee, S, Huang, M T, Kaproth   +3 more
openaire   +2 more sources

Spermatogenesis In Vitro: Searching for In Vivo Correlates

1987
The development of methodology for the study of spermatogenesis in vitro has been a major task that remained elusive (Wolff and Haffen, 1965; Steinberger, 1975). Significant advances have been reported in the culture of meiotic cells of lilaceous plants (Hotta et al., 1966), and spermatogenic cells of Xenopus (Risley, 1983) and Drosophila (Liebrich ...
A L, Kierszenbaum, M, Abdullah, H, Ueda, L L, Tres   +3 more
openaire   +2 more sources

SPERMATOGENESIS IN VITRO: COMPLETION OF MEIOSIS AND EARLY SPERMIOGENESIS

Endocrinology, 1983
In vitro formation of haploid spermatids has not been convincingly demonstrated in mammals. To investigate this problem we selected defined segments of rat seminiferous tubules containing late pachytene and diakinetic primary spermatocytes (Stages XII and XIII of the cycle) for culture in a chemically defined medium.
M, Parvinen, W W, Wright, D M, Phillips, J P, Mather, N A, Musto, C W, Bardin   +5 more
openaire   +2 more sources

In Vitro Spermatogenesis Using an Organ Culture Technique

2012
Research on in vitro spermatogenesis has a long history and remained to be an unaccomplished task until very recently. In 2010, we succeeded in producing murine sperm from primitive spermatogonia using an organ culture method. The fertility of the sperm or haploid spermatids was demonstrated by microinsemination.
Tetsuhiro, Yokonishi, Takuya, Sato, Kumiko, Katagiri, Takehiko, Ogawa   +3 more
openaire   +2 more sources

Stage-specific approaches promote in vitro induction for spermatogenesis

In Vitro Cellular & Developmental Biology - Animal, 2018
Spermatogenesis in vitro has been demonstrated using spermatogonial stem cells (SSCs) in monolayer culture or testis tissue fragments in agarose-constructed three-dimensional (3-D) conditions. However, the low efficiency of gamete maturation and the lack of a novel induction platform have limited the progress of its use in further research and clinical
Hualin, Yi, Sa, Xiao, Yan, Zhang
openaire   +2 more sources

The Presence of Colony-Stimulating Factor-1 and Its Receptor in Different Cells of the Testis; It Involved in the Development of Spermatogenesis In Vitro

International Journal of Molecular Sciences, 2021
Eitan Lunenfeld, Mahmoud Huleihel, Eden Arazi   +2 more
exaly  

Involvement of Cytokines and Hormones in the Development of Spermatogenesis In Vitro from Spermatogonial Cells of Cyclophosphamide-Treated Immature Mice

International Journal of Molecular Sciences, 2021
Josephk Kapelushnik, Eitan Lunenfeld, Mahmoud Huleihel   +2 more
exaly  

In Vitro Spermatogenesis

2018
Yukiko Ishikura, Mitinori Saitou
openaire   +1 more source