Results 51 to 60 of about 681,576 (264)

Modeling Melanoma In Vitro and In Vivo [PDF]

Healthcare, 2013
The behavior of melanoma cells has traditionally been studied in vitro in two-dimensional cell culture with cells adhering to plastic dishes. However, in order to mimic the three-dimensional architecture of a melanoma, as well as its interactions with the tumor microenvironment, there has been the need for more physiologically relevant models.
Beaumont, Kimberley A., Mohana-Kumaran, Nethia, Haass, Nikolas K.   +2 more
openaire   +5 more sources

A Novel Ex Vivo Model to Investigate the Underlying Mechanisms in Alzheimer’s Disease

Frontiers in Cellular Neuroscience, 2017
Currently there is no widely accepted animal model reproducing the full pathological profile of Alzheimer’s disease (AD), since the basic mechanisms of neurodegeneration are still poorly understood.
E. Brai, S. Stuart, Antoine-Scott Badin, S. Greenfield   +3 more
semanticscholar   +1 more source

Organoids in pediatric cancer research

FEBS Letters, EarlyView.
Organoid technology has revolutionized cancer research, yet its application in pediatric oncology remains limited. Recent advances have enabled the development of pediatric tumor organoids, offering new insights into disease biology, treatment response, and interactions with the tumor microenvironment.
Carla Ríos Arceo, Jarno Drost
wiley   +1 more source

Dense-UNet: a novel multiphoton in vivo cellular image segmentation model based on a convolutional neural network.

Quantitative Imaging in Medicine and Surgery, 2020
Background Multiphoton microscopy (MPM) offers a feasible approach for the biopsy in clinical medicine, but it has not been used in clinical applications due to the lack of efficient image processing methods, especially the automatic segmentation ...
Sijing Cai, Yunxian Tian, H. Lui, H. Zeng, Yi Wu, Guannan Chen   +5 more
semanticscholar   +1 more source

Reciprocal control of viral infection and phosphoinositide dynamics

FEBS Letters, EarlyView.
Phosphoinositides, although scarce, regulate key cellular processes, including membrane dynamics and signaling. Viruses exploit these lipids to support their entry, replication, assembly, and egress. The central role of phosphoinositides in infection highlights phosphoinositide metabolism as a promising antiviral target.
Marie Déborah Bancilhon, Bruno Mesmin
wiley   +1 more source

Modeling protein folding in vivo [PDF]

Biology Direct, 2018
A half century of studying protein folding in vitro and modeling it in silico has not provided us with a reliable computational method to predict the native conformations of proteins de novo, let alone identify the intermediates on their folding pathways.
Irina Sorokina, Arcady Mushegian
openaire   +3 more sources

Vascularized human cortical organoids (vOrganoids) model cortical development in vivo

PLoS Biology, 2020
Modeling the processes of neuronal progenitor proliferation and differentiation to produce mature cortical neuron subtypes is essential for the study of human brain development and the search for potential cell therapies. We demonstrated a novel paradigm
Yingchao Shi, Le Sun, Mengdi Wang, Jianwei Liu, Suijuan Zhong, Rui Li, Peng Li, Lijie Guo, Ai Fang, Ruiguo Chen, Woo-ping Ge, Qian Wu, Xiaoqun Wang   +12 more
semanticscholar   +1 more source

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

Development of experimental models of reactivated toxoplasmosis [PDF]

Medicinski Podmladak
Toxoplasmosis is one of the most widespread zoonosis worldwide, since Toxoplasma gondii (T. gondii) is a ubiquitous obligate intracellular protozoan parasite that infects up to one third of the human population. Although infection is usually asymptomatic
Bauman Neda, Srbljanović Jelena, Čolović-Čalovski Ivana   +2 more
doaj   +1 more source

Murine Precision-Cut Kidney Slices as an ex vivo Model to Evaluate the Role of Transforming Growth Factor-β1 Signaling in the Onset of Renal Fibrosis

Frontiers in Physiology, 2017
Renal fibrosis is characterized by progressive accumulation of extracellular matrix (ECM) proteins, resulting in loss of organ function and eventually requiring renal replacement therapy.
Elisabeth G. D. Stribos, M. Seelen, H. van Goor, P. Olinga, H. Mutsaers   +4 more
semanticscholar   +1 more source