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Structure-Function Relationship of Inclusion Bodies of a Multimeric Protein
Frontiers in Microbiology, 2020 High level expression of recombinant proteins in bacteria often results in their aggregation into inclusion bodies. Formation of inclusion bodies poses a major bottleneck in high-throughput recovery of recombinant protein.
Anupam Singh +3 more
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Artificial Inclusion Bodies for Clinical Development
Advanced Science, 2020 Bacterial inclusion bodies (IBs) are mechanically stable protein particles in the microscale, which behave as robust, slow‐protein‐releasing amyloids. Upon exposure to cultured cells or upon subcutaneous or intratumor injection, these protein materials ...
Julieta M. Sánchez +11 more
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Isolation of cell-free bacterial inclusion bodies [PDF]
Microbial Cell Factories, 2010 Background Bacterial inclusion bodies are submicron protein clusters usually found in recombinant bacteria that have been traditionally considered as undesirable products from protein production processes.
Rodríguez-Carmona Escarlata +4 more
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Morphogenesis and functional organization of viral inclusion bodies [PDF]
Cell Insight, 2023 Eukaryotic viruses are obligate intracellular parasites that rely on the host cell machinery to carry out their replication cycle. This complex process involves a series of steps, starting with virus entry, followed by genome replication, and ending with
Zhifei Li +4 more
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Tailoring the properties of (catalytically)-active inclusion bodies
Microbial Cell Factories, 2019 Background Immobilization is an appropriate tool to ease the handling and recycling of enzymes in biocatalytic processes and to increase their stability. Most of the established immobilization methods require case-to-case optimization, which is laborious
V. D. Jäger +13 more
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State-of-the-art and novel approaches to mild solubilization of inclusion bodies [PDF]
Frontiers in Bioengineering and Biotechnology, 2023 Throughout the twenty-first century, the view on inclusion bodies (IBs) has shifted from undesired by-products towards a targeted production strategy for recombinant proteins.
Robert Klausser +11 more
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Quality control of inclusion bodies in
Microbial Cell Factories, 2010 Background Bacterial inclusion bodies (IBs) are key intermediates for protein production. Their quality affects the refolding yield and further purification.
Schweder Thomas +7 more
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Microbial Cell Factories, 2018 Background Physiological aggregation of a recombinant enzyme into enzymatically active inclusion bodies could be an excellent strategy to obtain immobilized enzymes for industrial biotransformation processes.
Romana Koszagova +7 more
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Neurodegenerative disease-associated inclusion bodies are cleared by selective autophagy in budding yeast [PDF]
Autophagy Reports, 2023 Protein misfolding, aggregation, and accumulation cause neurodegenerative disorders. One such disorder, Huntington’s disease, is caused by an increased number of glutamine-encoding trinucleotide repeats CAG in the first exon of the huntingtin (HTT) gene.
Austin Folger +4 more
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Neuroserpin Inclusion Bodies in a FENIB Yeast Model [PDF]
Microorganisms, 2021 FENIB (familial encephalopathy with neuroserpin inclusion bodies) is a human monogenic disease caused by point mutations in the SERPINI1 gene, characterized by the intracellular deposition of polymers of neuroserpin (NS), which leads to proteotoxicity ...
Valentina Vapore +9 more
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